Spatially resolved proteomics of the Arabidopsis stomatal lineage identifies polarity complexes for cell divisions and stomatal pores
Developmental Cell,
Год журнала:
2024,
Номер
59(9), С. 1096 - 1109.e5
Опубликована: Март 21, 2024
Cell
polarity
is
used
to
guide
asymmetric
divisions
and
create
morphologically
diverse
cells.
We
find
that
two
oppositely
oriented
cortical
domains
present
during
the
in
Arabidopsis
stomatal
lineage
are
reconfigured
into
polar
marking
ventral
(pore-forming)
outward-facing
of
maturing
guard
Proteins
define
these
opposing
were
as
baits
miniTurboID-based
proximity
labeling.
Among
differentially
enriched
proteins,
we
kinases,
putative
microtubule-interacting
SOSEKIs
with
their
effector
ANGUSTIFOLIA.
Using
AI-facilitated
protein
structure
prediction
models,
identify
potential
protein-protein
interaction
interfaces
among
them.
Functional
localization
analyses
OPL2
its
partners
suggest
a
positive
mitotic
microtubules
role
cytokinesis.
This
combination
proteomics
structural
modeling
live-cell
imaging
provides
insights
how
rewired
different
cell
types
cell-cycle
stages.
Язык: Английский
Structural basis of human VANGL-PRICKLE interaction
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Янв. 3, 2025
Planar
cell
polarity
(PCP)
is
an
evolutionarily
conserved
process
for
development
and
morphogenesis
in
metazoans.
The
well-organized
pattern
cells
established
by
the
asymmetric
distribution
of
two
core
protein
complexes
on
opposite
sides
membrane.
Van
Gogh-like
(VANGL)-PRICKLE
(PK)
pair
one
these
key
regulators;
however,
their
structural
information
detailed
functions
have
been
unclear.
Here,
we
present
five
cryo-electron
microscopy
structures
human
VANGL1,
VANGL2,
with
PK1
at
resolutions
2.2–3.0
Å.
Through
biochemical
imaging
experiments,
decipher
molecular
details
VANGL-PK
interaction.
Furthermore,
reveal
that
can
target
VANGL-containing
intracellular
vesicles
to
peripheral
These
findings
provide
a
solid
foundation
understand
explicit
interaction
between
VANGL
PK
while
opening
new
avenues
subsequent
studies
PCP
pathway.
authors
VANGL2
PK1,
unveiling
underpinnings
paving
way
future
investigations
into
planar
pathway
Язык: Английский
Small Extracellular Vesicles Promote Axon Outgrowth by Engaging the Wnt-Planar Cell Polarity Pathway
Cells,
Год журнала:
2025,
Номер
14(1), С. 56 - 56
Опубликована: Янв. 6, 2025
In
neurons,
the
acquisition
of
a
polarized
morphology
is
achieved
upon
outgrowth
single
axon
from
one
several
neurites.
Small
extracellular
vesicles
(sEVs),
such
as
exosomes,
diverse
sources
are
known
to
promote
neurite
and
thus
may
have
therapeutic
potential.
However,
effect
fibroblast-derived
exosomes
on
elongation
in
neurons
central
nervous
system
under
growth-permissive
conditions
remains
unclear.
Here,
we
show
that
sEVs
neuronal
mouse
primary
embryonic
cortical
neurons.
Mechanistically,
demonstrate
sEV-induced
increase
requires
endogenous
Wnts
core
PCP
components
including
Prickle,
Vangl,
Frizzled,
Dishevelled.
We
internalized
by
colocalize
with
Wnt7b,
induce
relocalization
Vangl2
distal
during
outgrowth.
contrast,
derived
or
astrocytes
do
not
outgrowth,
while
activated
inhibit
elongation.
Thus,
our
data
reveal
through
Wnt-PCP
pathway
manner
dependent
Wnts.
Язык: Английский
Molecular and cellular processes underlying Unverricht-Lundborg disease—prospects for early interventions and a cure
Exploration of neuroscience,
Год журнала:
2024,
Номер
3(4), С. 295 - 308
Опубликована: Июль 18, 2024
A
short
overview
of
the
main
features
progressive
myoclonus
epilepsies
(PMEs),
such
as
Lafora
disease
(LD),
neuronal
ceroid
lipofuscinoses
(NCLs),
and
epilepsy
with
ragged-red
fibers
(MERRF)
is
given.
The
stress
this
review
paper
put
on
one
PME’s,
Unverricht-Lundborg
(ULD)—EPM1,
which
caused
by
mutations
in
human
cystatin
B
gene
(stefin
an
alternative
protein’s
name).
However,
different
other
genes/proteins
were
found
mutated
patients
presenting
EPM1-like
symptoms.
By
understanding
their
function
pathophysiological
roles,
further
insights
into
underlying
processes
EPM1
can
be
obtained.
On
a
broader
scale,
common
mechanisms
exist
between
ULD,
LD
NCLs,
as,
reactive
glia,
synaptic
remodeling,
hyperexcitability,
impairements
lysosomal/endocytosis
system,
cytoskeletal
functions,
mitochondria.
Oxidative
also
common.
molecular
cellular
processes,
early
interventions,
better
therapies
eventually,
using
modern
stem
cell,
editing
or
replacement
methods,
cure
expected.
Язык: Английский
Nuclear VANGL2 Inhibits Lactogenic Differentiation
Cells,
Год журнала:
2024,
Номер
13(3), С. 222 - 222
Опубликована: Янв. 25, 2024
Planar
cell
polarity
(PCP)
proteins
coordinate
tissue
morphogenesis
by
governing
patterning
and
polarity.
Asymmetrically
localized
on
the
plasma
membrane
of
cells,
transmembrane
PCP
are
trafficked
endocytosis,
suggesting
they
may
have
intracellular
functions
that
dependent
or
independent
their
extracellular
role,
but
whether
these
extend
to
transcriptional
control
remains
unknown.
Here,
we
show
nuclear
localization
transmembrane,
protein,
VANGL2,
in
HCC1569
breast
cancer
line,
undifferentiated,
not
differentiated,
HC11
cells
serve
as
a
model
for
mammary
lactogenic
differentiation.
The
loss
Vangl2
function
results
upregulation
pathways
related
STAT5
signaling.
We
identify
DNA
binding
sites
signal
use
CUT&RUN
demonstrate
recruitment
VANGL2
specific
motifs,
including
one
Stat5a
promoter.
Knockdown
(KD)
primary
organoids
Stat5a,
Ccnd1
Csn2,
larger
acini
organoids,
precocious
differentiation;
phenotypes
rescued
overexpression
Vangl2,
Vangl2ΔNLS.
Together,
advance
paradigm
whereby
keeping
programs
differentiation
check.
Язык: Английский
The Planar Cell Polarity Protein Fat1 in Sertoli Cell Function
Endocrinology,
Год журнала:
2024,
Номер
165(6)
Опубликована: Март 30, 2024
Abstract
Fat
(FAT
atypical
cadherin)
and
Dchs
(Dachsous
cadherin–related
protein)
in
adjacent
Sertoli:Sertoli,
Sertoli:spermatid,
spermatid:spermatid
interfaces
create
an
important
intercellular
bridge
whose
adhesive
function
is
turn
supported
by
Fjx1,
a
nonreceptor
Ser/Thr
protein
kinase.
This
concept
derived
from
earlier
studies
of
Drosophila,
which
has
been
confirmed
this
reports
as
well.
Herein,
we
use
the
approach
knockdown
Fat1
RNAi
using
primary
cultures
Sertoli
cells
that
mimicked
blood–testis
barrier
(BTB)
vivo,
series
coherent
experiments
including
functional
assays
to
monitor
cell
tight
junction
(TJ)
permeability
vitro
TJ
integrity
assay
assess
role
testis.
It
was
shown
planar
polarity
(PCP)
affected
through
its
modulation
actin
microtubule
cytoskeletal
function,
altering
their
polymerization
activity
Fat1/Fjx1
complex.
Furthermore,
intimately
associated
with
β-catenin
α-N-catenin,
well
Prickle
1
Vangl1/Prickle
complex,
another
PCP
core
support
interactions
confer
PCP.
In
summary,
these
findings
notion
Fat:Dchs
Vangl2:Fzd
bridges
are
tightly
basal
ES/TJ
structural
proteins
stabilize
at
interface
sustain
spermatogenesis.
Язык: Английский
Differential Sensitivity of Midline Patterning to Mitosis during and after Primitive Streak Extension
Опубликована: Окт. 26, 2024
Abstract
Background
Midline
establishment
is
a
fundamental
process
during
early
embryogenesis
for
Bilaterians
.
patterning
in
nonamniotes
can
occur
without
mitosis,
through
Planar
Cell
Polarity
(PCP)
signaling.
By
contrast,
amniotes
utilize
both
cell
proliferation
and
PCP
signaling
midline
landmark,
the
primitive
streak
(PS).
This
study
examined
their
roles
at
post
PS-extension.
Results
In
contrast
to
PS
extension
stages,
embryos
under
mitotic
arrest
PS-extension
preserved
notochord
(NC)
Hensen’s
node
(HN)/PS
regression
judged
by
morphology
marker
genes,
although
they
became
shorter,
laterality
was
lost.
Remarkably,
no
or
background
level
of
expression
detected
majority
core
components
NC-HN-PS
area
except
robustly
prickle-1
Morpholino
knockdown
Prickle-1
showed
little
influence
on
patterning,
suppressed
embryonic
growth.
Lastly,
associated
with
arrest-induced
size
reduction,
tissue
cells
displayed
hypertrophy.
Conclusion
Thus,
has
identified
least
two
distinct
mitosis
sensitivity
phases
pattering:
One
that
requires
PCP,
other
arrest-resistant
stages.
Язык: Английский
Exosomes promote axon outgrowth by engaging the Wnt-Planar Cell Polarity pathway
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 28, 2023
In
neurons,
the
acquisition
of
a
polarized
morphology
is
achieved
upon
outgrowth
single
axon
from
one
several
neurites.
Exosomes
or
small
extracellular
vesicles
(sEVs)
diverse
sources
are
known
to
promote
neurite
and
thus
may
have
therapeutic
potential.
However,
effect
fibroblast-derived
exosomes
on
elongation
in
neurons
central
nervous
system
under
growth
permissive
conditions
remains
unclear.
Here,
we
show
that
sEVs
neuronal
mouse
primary
embryonic
cortical
neurons.
Mechanistically,
demonstrate
sEV-induced
increase
requires
endogenous
Wnts
core
PCP
components
including
Prickle,
Vangl,
Frizzled
Dishevelled.
We
internalized
by
colocalize
with
Wnt7b
induce
relocalization
Vangl2
distal
during
outgrowth.
contrast,
derived
astrocytes
do
not
outgrowth,
while
activated
inhibit
elongation.
Thus,
our
data
reveals
through
Wnt-PCP
pathway
manner
dependent
Wnts.
Язык: Английский
Spatially resolved proteomics of the stomatal lineage: polarity complexes for cell divisions and stomatal pores
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 3, 2023
Abstract
Cell
polarity
is
used
to
guide
asymmetric
divisions
and
create
morphologically
diverse
cells.
We
find
that
two
oppositely
oriented
cortical
domains
present
during
the
in
Arabidopsis
stomatal
lineage
are
reconfigured
into
polar
marking
ventral
(pore-forming)
outward
facing
of
maturing
guard
Proteins
define
these
opposing
were
as
baits
miniTurboID-based
proximity
labeling.
Among
differentially
enriched
proteins
we
kinases,
putative
microtubule-interacting
proteins,
SOSEKIs
with
their
effector
ANGUSTIFOLIA,
using
AI-facilitated
protein
structure
prediction
models,
identify
potential
interaction
interfaces.
Functional
localization
analysis
OPL2
its
newly
discovered
partners
suggest
a
positive
mitotic
microtubules
role
cytokinesis.
This
combination
cutting-edge
proteomics
structural
modeling
live
cell
imaging
provides
insights
how
rewired
different
types
cycle
stages.
Язык: Английский
Nuclear VANGL2 Inhibits Lactogenic Differentiation
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 8, 2023
ABSTRACT
Planar
cell
polarity
(PCP)
proteins
coordinate
tissue
morphogenesis
by
governing
patterning
and
polarity.
Asymmetrically
localized
on
the
plasma
membrane
of
cells,
PCP
are
also
trafficked
endocytosis,
suggesting
they
may
have
intracellular
functions
that
dependent
or
independent
their
extracellular
role,
but
whether
these
extend
to
transcriptional
control
remains
unknown.
Here,
we
show
nuclear
localization
transmembrane,
protein,
VANGL2,
in
undifferentiated,
not
differentiated,
HC11
which
serve
as
a
model
for
mammary
lactogenic
differentiation.
Loss
Vangl2
function
results
upregulation
pathways
related
STAT5
signaling.
We
identify
DNA
binding
sites
signal
use
CUT&RUN
demonstrate
direct
VANGL2
specific
motifs,
including
one
Stat5a
promoter.
Knockdown
(KD)
cells
primary
organoids
,
Ccnd1
Csn2
larger
acini
organoids,
precocious
differentiation;
phenotypes
rescued
overexpression
ΔNLS
.
Together,
advance
paradigm
whereby
keeping
programs
differentiation
check.
Язык: Английский