M2 macrophage-derived exosomes reverse TGF-β1-induced epithelial mesenchymal transformation in BEAS-2B cells via the TGF-βRI/Smad2/3 signaling pathway DOI Creative Commons
Chao Liu, Xiaolin Huang, Siqi Li

и другие.

European journal of medical research, Год журнала: 2025, Номер 30(1)

Опубликована: Апрель 11, 2025

Airway remodeling in bronchial asthma can be inhibited by disrupting the epithelial mesenchymal transition (EMT) of activated airway cells. Exosomes, as key mediators intercellular communication, have been implicated pathophysiology asthma-related inflammation, remodeling, and hyperresponsiveness. This study aimed to investigate role M2 macrophage-derived exosomes (M2φ-exos) modulating TGF-β1-induced EMT (BEAS-2B) cells elucidate underlying molecular mechanism, if any. THP-1 were induced differentiate into macrophages via phorbol 12-myristate 13-acetate (PMA) IL-4. Exosomes subsequently isolated purified ultracentrifugation. M2φ-exos expression was characterized protein marker levels, transmission electron microscopy imaging, nanoparticle tracking analysis. BEAS-2B exposed determine latter's effects. successfully differentiated macrophages, confirmed vitro flow cytometry. The presented typical cup-shaped structures expressed CD81 TSG101. TGF-β1 induction altered morphological characteristics TGF-βRI/Smad2/3 signaling pathway, leading increased Snail, Vimentin Collagen 1 decreased E-cadherin. After exosome or SB431542 induction, reversed. GW4869, an release inhibitor, exhibited ability block beneficial effects exosomes. M2Φ-exos through pathway. novel insight may important implications for asthma, particularly addressing remodeling.

Язык: Английский

NK cellular derived nanovesicles in tumor immunity DOI Creative Commons

Dingru Li,

Yixin Shi, Sifei Yu

и другие.

Molecular Immunology, Год журнала: 2025, Номер 182, С. 54 - 61

Опубликована: Апрель 4, 2025

Natural Killer (NK) cells are a vital element of the innate immune system, and NK cell-based therapies have demonstrated efficacy against various malignancies. However, targeting solid tumors has been challenging due to low infiltration into effective evasion strategies employed by tumors. Recent studies shown that cell derived nanovesicles (NK-NV) can not only replicate functions but also offer more advantages in clinical applications. They capable transporting cellular components such as proteins, nucleic acids, lipids across distances, thereby facilitating intercellular communication among within tumor microenvironment (TME). With progress nanomedical technology, these vesicles be engineered carry range functional elements therapeutic agents enhance their antitumoral capabilities. In this review, we summarize current available literature on NK-NVs, discuss potential biological role non-coding RNAs (ncRNAs), explore application treatment

Язык: Английский

Процитировано

1

The relationship of miR-155 host gene polymorphism in the susceptibility of cancer: a systematic review and meta-analysis DOI Creative Commons

Gang Jin,

Tao Guo, Jiawei Liu

и другие.

Frontiers in Genetics, Год журнала: 2025, Номер 16

Опубликована: Март 6, 2025

miR-155 is overexpressed in many cancers, highlighting its potential as a biomarker for cancer diagnosis, treatment, and therapeutic evaluation. processed from the host gene (MIR155HG). Genetic variations MIR155HG may influence susceptibility, but existing evidence inconclusive. This study aimed to evaluate association of polymorphisms with risk. A systematic literature search identified 15 case-control studies on three single nucleotide (SNPs): rs767649 (T > A), rs928883 (G rs1893650 C). Meta-analysis was performed using RevMan 5.4, odds ratios (ORs) 95% confidence intervals (CIs) effect measures. No significant observed overall analysis. However, subgroup analysis revealed increased susceptibility respiratory, digestive, reproductive while reducing risk after excluding cancers. showed protective digestive not significantly associated specific subtypes, particularly respiratory These findings underscore importance genetic environmental factors warrant further investigation.

Язык: Английский

Процитировано

0

M2 macrophage-derived exosomes reverse TGF-β1-induced epithelial mesenchymal transformation in BEAS-2B cells via the TGF-βRI/Smad2/3 signaling pathway DOI Creative Commons
Chao Liu, Xiaolin Huang, Siqi Li

и другие.

European journal of medical research, Год журнала: 2025, Номер 30(1)

Опубликована: Апрель 11, 2025

Airway remodeling in bronchial asthma can be inhibited by disrupting the epithelial mesenchymal transition (EMT) of activated airway cells. Exosomes, as key mediators intercellular communication, have been implicated pathophysiology asthma-related inflammation, remodeling, and hyperresponsiveness. This study aimed to investigate role M2 macrophage-derived exosomes (M2φ-exos) modulating TGF-β1-induced EMT (BEAS-2B) cells elucidate underlying molecular mechanism, if any. THP-1 were induced differentiate into macrophages via phorbol 12-myristate 13-acetate (PMA) IL-4. Exosomes subsequently isolated purified ultracentrifugation. M2φ-exos expression was characterized protein marker levels, transmission electron microscopy imaging, nanoparticle tracking analysis. BEAS-2B exposed determine latter's effects. successfully differentiated macrophages, confirmed vitro flow cytometry. The presented typical cup-shaped structures expressed CD81 TSG101. TGF-β1 induction altered morphological characteristics TGF-βRI/Smad2/3 signaling pathway, leading increased Snail, Vimentin Collagen 1 decreased E-cadherin. After exosome or SB431542 induction, reversed. GW4869, an release inhibitor, exhibited ability block beneficial effects exosomes. M2Φ-exos through pathway. novel insight may important implications for asthma, particularly addressing remodeling.

Язык: Английский

Процитировано

0