Network Pharmacology-Guided Evaluation of Ginger and Cornelian Cherry Extracts Against Depression and Metabolic Dysfunction in Estrogen-Deficient Chronic Stressed Rats DOI Open Access
N. Lee, Ting Zhang,

Hanbin Joe

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(10), С. 4829 - 4829

Опубликована: Май 18, 2025

This study investigated the therapeutic effects of water extracts from Zingiber officinale Roscoe (ginger) and Cornus officinalis Siebold Zucc. fruits (COF) on depression-like behavior metabolic dysfunction in estrogen-deficient rats exposed to chronic mild stress (CMS). Network pharmacology analysis identified three bioactive compounds ginger four COF, with 11 overlapping targets linked both depression pathways, primarily involving NR3C1, HTR2A, MAOA, SLC6A4 genes associated hypothalamic–pituitary–adrenal (HPA) axis regulation neurotransmitter modulation. Ovariectomized received 200 mg/kg/day or COF for 7 weeks, a 4-week CMS protocol initiated at week 3. Both significantly improved behaviors, memory performance, glucose tolerance, lipid profiles, bone mineral density, normalized HPA markers (corticosterone ACTH), increased hippocampal serotonin dopamine levels. Ginger demonstrated greater efficacy improving outcomes compared COF. Molecular docking further validated these findings, revealing strong stable interactions between key phytochemicals—such as hydroxygenkwanin telocinobufagin—and target proteins supporting their mechanistic role mood regulation. These results support supplementing promising botanical interventions estrogen-deficiency-related disorders, potential clinical application human-equivalent dose 1.5 g/day.

Язык: Английский

Network Pharmacology-Guided Evaluation of Ginger and Cornelian Cherry Extracts Against Depression and Metabolic Dysfunction in Estrogen-Deficient Chronic Stressed Rats DOI Open Access
N. Lee, Ting Zhang,

Hanbin Joe

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(10), С. 4829 - 4829

Опубликована: Май 18, 2025

This study investigated the therapeutic effects of water extracts from Zingiber officinale Roscoe (ginger) and Cornus officinalis Siebold Zucc. fruits (COF) on depression-like behavior metabolic dysfunction in estrogen-deficient rats exposed to chronic mild stress (CMS). Network pharmacology analysis identified three bioactive compounds ginger four COF, with 11 overlapping targets linked both depression pathways, primarily involving NR3C1, HTR2A, MAOA, SLC6A4 genes associated hypothalamic–pituitary–adrenal (HPA) axis regulation neurotransmitter modulation. Ovariectomized received 200 mg/kg/day or COF for 7 weeks, a 4-week CMS protocol initiated at week 3. Both significantly improved behaviors, memory performance, glucose tolerance, lipid profiles, bone mineral density, normalized HPA markers (corticosterone ACTH), increased hippocampal serotonin dopamine levels. Ginger demonstrated greater efficacy improving outcomes compared COF. Molecular docking further validated these findings, revealing strong stable interactions between key phytochemicals—such as hydroxygenkwanin telocinobufagin—and target proteins supporting their mechanistic role mood regulation. These results support supplementing promising botanical interventions estrogen-deficiency-related disorders, potential clinical application human-equivalent dose 1.5 g/day.

Язык: Английский

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