Identification of Submergence Tolerance Loci in Dongxiang Wild Rice (DXWR) by Genetic Linkage and Transcriptome Analyses

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 1829 - 1829

Опубликована: Фев. 20, 2025

The submergence tolerance of rice is a key factor in promoting direct seeding technology and resisting flood disasters. Dongxiang wild (DXWR) has strong tolerance, but its genetic basis still unclear. Here, we report quantitative trait loci (QTLs) analysis for hypoxic germination rate (HGR), seedling (HSR), budlet survival (BSSR) (SSSR) using linkage map the backcross recombinant inbred lines (BRILs) that were derived from cross DXWR, an indica cultivar, GZX49. A total 20 QTLs related to detected, explaining phenotypic variations ranging 2% 8.5%. Furthermore, transcriptome sequencing was performed on seeds seedlings DXWR before after submergence. During seed stages, 6306 3226 differentially expressed genes (DEGs) detected respectively. Gene ontology (GO) Kyoto encyclopedia genomes (KEGG) analyses conducted these genes. Using data, combined with qRT-PCR, sequence comparison, bioinformatics, LOC_Os05g32820 putatively identified as candidate gene qHGR5.2 co-located HGR SSSR. These results will provide insights into mechanism improving tolerance.

Язык: Английский

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Overexpression of the general transcription factor OsTFIIB5 alters rice development and seed quality

Plant Cell Reports, Год журнала: 2025, Номер 44(2)

Опубликована: Янв. 10, 2025

Язык: Английский

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Research Progress on a Wide and Narrow Row Cropping System for Crops

Agronomy, Год журнала: 2025, Номер 15(1), С. 248 - 248

Опубликована: Янв. 20, 2025

Optimizing planting density enhances light capture, improves air circulation, and promotes more efficient resource utilization, ultimately leading to increased crop productivity. It facilitates uniform growth, maximizes land use efficiency, reduces nutrient competition, supports sustainable weed management, thereby improving yield efficiency. The wide narrow row cropping (WNRC) system is an optimized method that adjusts the spacing strategically enhance growth This study reviews development implementation of WNRC technology, focusing on its effects development, environmental optimization. (1) Crop optimization: Modifying in interception, soil moisture distribution, creating environment photosynthetic efficiency water use. (2) Genetic variation performance: performance different varieties systems varies, with specific showing better adaptation altered spatial arrangement, improved uniformity higher yields. (3) Weed management: optimized, reducing need for herbicides fostering control methods. (4) Efficient input application fertilizers pesticides, optimizing uptake, minimizing wastage, lowering impact. While offers substantial advantages enhancement optimization, challenges remain adapting this technology diverse conditions. Further research required refine regions crops, ensuring long-term agronomic ecological benefits.

Язык: Английский

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Design and experiment of impeller seed guide device for rice internal suction hole direct seeding device

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Март 20, 2024

Hole seeding and uniform hole spacing are important agronomic evaluation indexes of rice precision hill-direct-seeding metering device. Based on the principle internal suction seeding, this paper innovatively designs an impeller The main structural parameters seed guide device were analyzed by constructing kinematics model in process. effects radius, blade offset angle performance studied bench test. seed-guiding experiments different sizes seeds show that has better hole-forming than traditional tube. guiding proposed can improve fixed-distance multi-grain hill-seeding tubular device, it provides a reference for design bunching crop

Язык: Английский

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OsRAV1 Regulates Seed Vigor and Salt Tolerance During Germination in Rice

Rice, Год журнала: 2024, Номер 17(1)

Опубликована: Сен. 2, 2024

Seed vigor is a complex trait encompassing seed germination, seedling emergence, growth, longevity, and stress tolerance, all are crucial for direct seeding in rice. Here, we report that the AP2/ERF transcription factor OsRAV1 (RELATED TO ABI3 AND VP1) positively regulates vigor, salt tolerance. Additionally, was differently expressed embryo endosperm, with localized nucleus. Transcriptomic analysis revealed modulates through plant hormone signal transduction phenylpropanoid biosynthesis during germination. Haplotype showed rice varieties carrying Hap3 displayed enhanced tolerance These findings suggest potential target breeding high suitable cultivation.

Язык: Английский

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Adaptations of Rice Seed Germination to Drought and Hypoxic Conditions: Molecular and Physiological Insights

Seeds, Год журнала: 2024, Номер 3(4), С. 656 - 676

Опубликована: Дек. 2, 2024

Seed germination is crucial for plant survival, crop stand establishment, and achieving optimal grain yield. The main objective of this review to explore the physiological molecular mechanisms governing rice seed under aerobic (water stress) anaerobic (hypoxic) conditions in direct-seeded (DSR) systems. Moreover, it discusses recent genomic advancements innovations improve germination. Here, we discuss how coleoptile mesocotyl elongation plays a vital role (AG) function raised antioxidants, including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) maintaining Reactive Oxygen Species (ROS), malondialdehyde (MDA) homeostasis stabilizing water-scarce conditions. This study comprehensively highlights functions dynamics phytohormones—GA (gibberellic acid) ABA (abscisic acid)—key regulatory genes, transcription factors (TFs), key proteins, metabolic pathways, glycolysis, pentose phosphate pathway (PPP), tricarboxylic acid cycle (TCA), regulating both Conventional agronomic cultural practices, such as selection, priming, coating, hardening, have proven utilization novel approaches—such clustered regularly interspaced short palindromic repeat (CRISPR-Cas9) mediated genome editing, marker-assisted selection (MAS), genome-wide associations studies (GWAS), single nucleotide polymorphisms (SNPs), multi-omics, RNA sequencing—combined with beneficial quantitative trait loci (QTLs) has expanded knowledge genomics inheritance. These aid development specific traits enhancing DSR.

Язык: Английский

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The Function of SD1 on Shoot Length and its Pyramiding Effect on Shoot Length and Plant Height in Rice (Oryza sativa L.)

Rice, Год журнала: 2024, Номер 17(1)

Опубликована: Март 25, 2024

Strong seedling vigor is imperative to achieve stable establishment and enhance the competitiveness against weeds in rice direct seeding. Shoot length (SL) one of important traits associated with rice, but few genes for SL have been cloned so far. In previous study, we identified two tightly linked stably expressed QTLs SL, qSL-1f qSL-1d by genome-wide association causal gene (LOC_Os01g68500) underlying qSL-1f. present identify LOC_Os01g66100 (i.e. semidwarf SD1), a well-known controlling plant height (PH) at adult-plant stage, as through gene-based haplotype analysis knockout transgenic verification. By measuring phenotypes (SL PH) various haplotypes their lines, found SD1 LOC_ Os01g68500 controlled both PH, worked same direction, which provided directly genetic evidence positive correlation between PH combined diverse natural population. Moreover, experiments suggested that had greater effect on compared Os01g68500, no significant difference SL. Further investigation pyramiding effects LOC_Os01g68500 based combinations may play dominant role when coexist. this stage validated. total, genes, are our studies, suitable beneficial desired different breeding objectives. These results provide new clue develop varieties seeding resources molecular strong vigor.

Язык: Английский

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Design and experiment of impeller seed guide device for rice internal suction hole direct seeding device

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Июнь 10, 2024

Язык: Английский

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A Novel Function of GW5 on Controlling the Early Growth Vigor and its Haplotype Effect on Shoot Dry Weight and Grain Size in Rice (Oryza sativa L.)

Rice, Год журнала: 2024, Номер 17(1)

Опубликована: Авг. 10, 2024

Strong early growth vigor is an essential target in both direct seeded rice breeding and high-yielding for varieties with relatively short duration the double-cropping region. Shoot dry weight (SDW) one of important traits associated vigor, breeders have been working to improve this trait. Finding stable QTLs or functional genes SDW crucial improving by implementing molecular rice. Here, a genome-wide association analysis revealed that QTL SDW, qSDW-5, was stably detected three cultivation methods commonly used production practice. Through gene-based haplotype annotated within putative region validated gene expression knockout transgenic experiments, LOC_Os05g09520, which identical reported GW5/GSE5 controlling grain width (GW) thousand (TGW) identified as causal qSDW-5. Five main haplotypes LOC_Os05g09520 were diverse international collection study their effects on GW TGW analyzed. Phenotypic comparisons major subpopulations (indica, japonica aus) same patterns wider higher along SDW. Furtherly, 138 varieties/lines widely southern China showed 97.8% cultivars/lines carry Hap2

Язык: Английский

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A point mutation in VIG1 boosts development and chilling tolerance in rice

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Сен. 18, 2024

Язык: Английский

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