BcAS2 Regulates Leaf Adaxial Polarity Development in Non-Heading Chinese Cabbage by Directly Activating BcPHB Transcription DOI Creative Commons
Jiang Cheng,

Qiang Ding,

Ying He

и другие.

Plants, Год журнала: 2025, Номер 14(8), С. 1207 - 1207

Опубликована: Апрель 14, 2025

Leaves are the primary organs for plant photosynthesis, and their flat, symmetric morphology is crucial growth development. The LBD family transcription factor ASYMMETRIC LEAVES 2 (AS2) plays a central role in establishment of leaf polarity. In this study, we cloned BcAS2 gene from non-heading Chinese cabbage cultivar “NHCC001” successfully generated overexpression strains through genetic transformation. Phenotypic analysis revealed that led to significant upward curling leaves cabbage. Additionally, found expression BcPHB, associated with adaxial polarity development, was significantly up-regulated BcAS2-overexpressing plants compared controls. This interaction further confirmed yeast one-hybridization (Y1H), dual-luciferase reporter assays, electrophoretic mobility shift assay (EMSA), all which demonstrated directly binds GATA-motif site BcPHB promoter promotes its transcription. Functional validation via silencing regulating development leaves. study elucidates molecular mechanism BcAS2-BcPHB pathway cabbage, providing theoretical foundation morphological improvement breeding.

Язык: Английский

BcAS2 Regulates Leaf Adaxial Polarity Development in Non-Heading Chinese Cabbage by Directly Activating BcPHB Transcription DOI Creative Commons
Jiang Cheng,

Qiang Ding,

Ying He

и другие.

Plants, Год журнала: 2025, Номер 14(8), С. 1207 - 1207

Опубликована: Апрель 14, 2025

Leaves are the primary organs for plant photosynthesis, and their flat, symmetric morphology is crucial growth development. The LBD family transcription factor ASYMMETRIC LEAVES 2 (AS2) plays a central role in establishment of leaf polarity. In this study, we cloned BcAS2 gene from non-heading Chinese cabbage cultivar “NHCC001” successfully generated overexpression strains through genetic transformation. Phenotypic analysis revealed that led to significant upward curling leaves cabbage. Additionally, found expression BcPHB, associated with adaxial polarity development, was significantly up-regulated BcAS2-overexpressing plants compared controls. This interaction further confirmed yeast one-hybridization (Y1H), dual-luciferase reporter assays, electrophoretic mobility shift assay (EMSA), all which demonstrated directly binds GATA-motif site BcPHB promoter promotes its transcription. Functional validation via silencing regulating development leaves. study elucidates molecular mechanism BcAS2-BcPHB pathway cabbage, providing theoretical foundation morphological improvement breeding.

Язык: Английский

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