Approaches for the amelioration of adverse effects of drought stress on soybean plants: from physiological responses to agronomical, molecular, and cutting-edge technologies

Plant and Soil, Год журнала: 2025, Номер unknown

Опубликована: Янв. 14, 2025

Язык: Английский

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An overview of heat stress in Chickpea (Cicer arietinum L.): effects, mechanisms and diverse molecular breeding approaches for enhancing resilience and productivity

Molecular Breeding, Год журнала: 2025, Номер 45(2)

Опубликована: Янв. 21, 2025

Язык: Английский

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Optimization of Prime Editing in Rice, Peanut, Chickpea, and Cowpea Protoplasts by Restoration of GFP Activity

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(17), С. 9809 - 9809

Опубликована: Авг. 29, 2022

Precise editing of the plant genome has long been desired for functional genomic research and crop breeding. Prime is a newly developed precise technology based on CRISPR-Cas9, which uses an engineered reverse transcriptase (RT), catalytically impaired Cas9 endonuclease (nCas9), prime guide RNA (pegRNA). In addition, wider range types than base can produce nearly all edits. Although was first established in human cells, it recently applied to plants. As relatively new technique, optimization will be needed increase efficiency different crops. this study, we successfully edited mutant GFP rice, peanut, chickpea, cowpea protoplasts. up 16 times higher achieved with dual pegRNA single containing vectors. Edited-mutant protoplasts have also obtained after transformation vectors, albeit much lower ranging from 0.2% 0.5%. These initial results promise expedite application legume breeding programs accelerate improvement.

Язык: Английский

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Enhancing Cowpea Tolerance to Elevated Temperature: Achievements, Challenges and Future Directions

Agronomy, Год журнала: 2024, Номер 14(3), С. 513 - 513

Опубликована: Март 1, 2024

Despite its ability to thrive in high-temperature environments, cowpea productivity can be hampered by heat stress, particularly when night air temperatures exceed 17 °C. The crop’s germplasm pool potentially possesses significant genetic variability that harnessed breed for heat-tolerant varieties. Progress improving the crop tolerance has been limited, especially under hot, short-day environments typical of sub-Saharan Africa. Only a few varieties have released, partly due limited understanding stress mechanisms and environmental interaction effects on genotypes, as well imprecise phenotyping. This review contributes literature highlighting key achievements, challenges, future directions breeding genotypes providing additional information from recent literature. We opine tolerance-related traits not sufficiently exploited developing adapted target production environments. Therefore, attention should given assessing repository targeting adaptive, morphological, physiological enhance tolerance. propose programs integrate phenotyping whole-plant molecular identify breeder-friendly markers routine selection. followed introgression favourable alleles susceptible using rapid precise approaches take advantage modern genomic resources such innovative resources, selection, speed breeding, genome editing technologies. These tools hold great promise fast-tracking development improved incorporating must-have preferred farmers consumers. In view likely increase atmospheric temperature occasioned climate change, there is an urgent need develop ensure sustainability current cropping agri-food systems.

Язык: Английский

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Improvement of heat stress tolerance in soybean (Glycine max L), by using conventional and molecular tools

Frontiers in Plant Science, Год журнала: 2022, Номер 13

Опубликована: Сен. 26, 2022

The soybean is a significant legume crop, providing several vital dietary components. Extreme heat stress negatively affects yield and quality, especially at the germination stage. Continuous change in climatic conditions threatening global food supply security. Therefore, it critical need of time to develop heat-tolerant genotypes. Different molecular techniques have been developed improve tolerance soybean, but until now complete genetic mechanism not fully understood. Various methods, like quantitative trait loci (QTL) mapping, engineering, transcription factors (TFs), transcriptome, clustered regularly interspaced short palindromic repeats (CRISPR), are employed incorporate under extreme stress. These significantly improved soybean. Besides this, we can also use specific classical breeding approaches different hormones reduce harmful consequences waves on In future, integrated these tools would bring results developing current review, presented detailed overview improvement highlighted future prospective. Further studies required investigate governing response This information be helpful for focusing improving

Язык: Английский

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Application of CRISPR–cas-based technology for the identification of tuberculosis, drug discovery and vaccine development

Molecular Biology Reports, Год журнала: 2024, Номер 51(1)

Опубликована: Март 29, 2024

Язык: Английский

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A Review on Seed Storage Technology: Recent Trends and Advances in Sustainable Techniques for Global Food Security

AgroEnvironmental Sustainability, Год журнала: 2024, Номер 2(1), С. 34 - 50

Опубликована: Март 25, 2024

Seed storage innovation assumes an essential part in guaranteeing worldwide food security by safeguarding genetic variety and preserving seed feasibility over time. This review article gives a complete outline of ongoing patterns advances supportable stockpiling strategies. Starting with investigation the significance about challenges, audit digs into conventional storing strategies their obstacles. Additionally also provides comprehensive analysis maintainable methodologies like controlled air capacity, cryopreservation, preparing, featuring adequacy dragging out suitability while diminishing energy utilization natural effect. Moreover, discusses incorporation digital technologies, for example, artificial intelligence blockchain, management to enhance traceability convenience collections. Additionally, role community-based banks participatory plant breeding promoting resilience against climate change is explored. By discussing current research findings practical applications, this aims inform policymakers, researchers, practitioners diverse range sustainable solutions available safeguard global agricultural biodiversity ensure face evolving ecological challenges.

Язык: Английский

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Fine-tuning CRISPR/Cas9 gene editing in common bean (Phaseolus vulgaris L.) using a hairy root transformation system and in silico prediction models

Frontiers in Plant Science, Год журнала: 2023, Номер 14

Опубликована: Окт. 20, 2023

The stable transformation of common bean is a challenging and time-consuming process. Although CRISPR/Cas9 has revolutionized gene editing with its high efficiency specificity, the performance system can be affected by multiple factors, such as sgRNA specificity effectiveness, choice promoter used to drive Cas9 expression. use hairy root initially check sgRNAs impact different promoters could speed up this process increase chances success. We tested three methods induce roots selected preferred method suitable for variety genotypes. This involved inoculating severed radicle Rhizobium rhizogenes K599 was fast, had frequency 42-48%, resulted in numerous roots. further explants using R. harboring constructs evaluated on-target activity targeting raffinose family oligosaccharides biosynthetic genes driving on efficiency. Additionally, we reliability silico tools, CRISPOR, CRISPR RGEN, inDelphi predict efficiencies resulting mutations. Our results showed that allows rapid evaluation promoters. also identified several highly efficient induced frameshift mutations at rates 70% when parsley ubiquitin expression, providing valuable information selection most effective future experiments. computational models did not match planta results, Lindel model proved reliable P. vulgaris, accurately predicting type mutation Furthermore, algorithm correctly deletions single nucleotide insertions from DNA double-strand breaks bean. These offer promising implications enhancing precise plants because they provide possibility repair outcomes.

Язык: Английский

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Unraveling Origin, History, Genetics, and Strategies for Accelerated Domestication and Diversification of Food Legumes

Frontiers in Genetics, Год журнала: 2022, Номер 13

Опубликована: Июль 22, 2022

Domestication is a dynamic and ongoing process of transforming wild species into cultivated by selecting desirable agricultural plant features to meet human needs such as taste, yield, storage, cultivation practices. Human domestication began in the Fertile Crescent around 12,000 years ago spread throughout world, including China, Mesoamerica, Andes Near Oceania, Sub-Saharan Africa, eastern North America. Indus valley civilizations have played great role grain legumes. Crops, pigeon pea, black gram, green lablab bean, moth horse originated Indian subcontinent, Neolithic archaeological records indicate that these crops were first domesticated early region. The evolution ancestors today’s elite cultivars are important contributors global food supply crop improvement. In addition, legumes contribute security protecting health minimize climate change impacts. During process, legume undergone severe genetic diversity loss, only very narrow range variability retained cultivars. Further reduction occurred during seed dispersal movement across continents. general, few traits, shattering resistance, dormancy stem growth behavior, flowering–maturity period, yield prominence species. Thus, identification knowledge responsive loci often useful accelerating new species’ domestication. genes metabolic pathways responsible for significant alterations an outcome might aid quick novel crops. Further, recent advances “omics” sciences, gene-editing technologies, functional analysis will accelerate improvement without losing much diversity. this review, we discussed about origin, center diversity, major legumes, which be exploration utilization genes/QTLs associated with syndrome pulse future strategies improve

Язык: Английский

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Enhancing Horticultural Crops through Genome Editing: Applications, Benefits, and Considerations

Horticulturae, Год журнала: 2023, Номер 9(8), С. 884 - 884

Опубликована: Авг. 3, 2023

Genome editing has emerged as a powerful tool for accelerating crop improvement in horticultural crops by enabling precise modifications to their genetic makeup. This review provides an in-depth exploration of the applications, methodologies, and potential impacts genome horticulture. The focuses on three major tools horticulture, CRISPR-Cas9, TALENs, ZFNs. underlying mechanisms, challenges associated with each are discussed detail. being versatile widely used system, enhance traits such disease resistance, abiotic stress tolerance, nutritional content, yield crops. TALENs ZFNs, although less commonly used, offer alternative options targeted DNA modifications, have demonstrated success specific applications. We emphasize benefits including improved productivity, quality, value. However, off-target effects, delivery methods, regulatory frameworks need be addressed full realization this technology’s potential. serves valuable resource researchers, policymakers, stakeholders, providing insights into opportunities complexities harnessing enhanced By navigating these challenges, can contribute sustainable advancements benefiting both producers consumers worldwide.

Язык: Английский

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