MiceDEGdb: a knowledge base on differentially expressed mouse genes as a model object in biomedical research

Vavilov Journal of Genetics and Breeding, Год журнала: 2025, Номер 29(1), С. 153 - 161

Опубликована: Март 4, 2025

The fundamental understanding of many biological processes that unfold in a human body has become possible due to experimental studies on animal models. backbone modern biomedical research is the use mouse models for studying important pathophysiological mechanisms, assessing new therapeutic approaches and making decisions acceptance or rejection candidate medicines preclinical trials. mice advantageous because they have small size, are easy keep genetically modify. Mice make up more than 90 % rodents used pharmaceutical research. We present pilot version MiceDEGdb, knowledge base genes differentially expressed as model object researc h. MiceDEGdb collection published data gene expression strains age-related diseases, such hypertension, pe rio dontal disease, bone fragility, renal fibrosis, smooth muscle remodeling, heart failure circadian rhythm disorder. release contains 21,754 DEGs representing 9,769 unique Mus musculus transcription levels whereof were found being changed 25 RNA-seq experiments involving eight tissues – gum, bone, kidney, right ventricle, aortic arch, hippocampus, skeletal uterus six genetic (C57BL/6J, Ren1cCre|ZsGreen, B6.129S7(Cg)-Polgtm1Prol/J, BPN/3J, BPH/2J Kunming) diseases all these based information 10 original articles. novel it features curated annotation changes using independent publications about same-direction homologs patients with one disease other. In its release, documented 85,092 annotations 318 895 suggest 912 scientific articles referenced by their PubMed ID. contained may be interest geneticists, molecular biologists, bioinformatics scientists, clinicians, pharmacologists advisors personalized medicine. freely available at https://www.sysbio.ru/MiceDEGdb .

Язык: Английский

Non-viral generation of transgenic non-human primates via the piggyBac transposon system

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Март 24, 2025

Abstract Non-human primates, such as cynomolgus monkeys, are invaluable experimental models for understanding human biology and disease. Their close genetic relationship to humans makes them essential studying fundamental developmental processes disease progression. Although lentiviral methods generating transgenic monkeys exist, several inherent technical difficulties limit their utility. To solve this problem, here we establish a non-viral method using the piggyBac transposon system. After optimizing our protocol in mice, show that co-injection of components with sperm into metaphase II-stage oocytes successfully generates expressing transgenes throughout whole bodies. Transgene expression is observed all examined tissue types, including germ cells, although levels vary. Insertion analysis further confirms successful integration transgene. We propose will be practical non-human primates.

Язык: Английский

Efficient genome editing of two-cell mouse embryos via modified CRISPR/Cas electroporation

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Дек. 5, 2024

Creating genetically modified (GM) animals using CRISPR/Cas mediated through the electroporation of two-cell stage embryos, rather than fertilized eggs, holds considerable potential. The full potential genome editing embryos is only beginning to be explored. We developed an improved method prevent blastomere fusion in two-cell-stage enabling efficient editing. Using this method, we demonstrated that indel mutation rates and ssODN knock-in (KI) efficiencies are comparable those with a tendency for higher efficiency long DNA KI. This study highlights value provides enhanced animal model production opportunities. Furthermore, realizing extends timeframe from egg embryo, offering promising avenues future research embryo techniques.

Язык: Английский