Next-Generation Probiotics and Chronic Diseases: A Review of Current Research and Future Directions

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(50), С. 27679 - 27700

Опубликована: Ноя. 26, 2024

The burgeoning field of microbiome research has profoundly reshaped our comprehension human health, particularly highlighting the potential probiotics and fecal microbiota transplantation (FMT) as therapeutic interventions. While benefits traditional are well-recognized, efficacy mechanisms remain ambiguous, FMT's long-term effects still being investigated. Recent advancements in high-throughput sequencing have identified gut microbes with significant health benefits, paving way for next-generation (NGPs). These NGPs, engineered through synthetic biology bioinformatics, designed to address specific disease states enhanced stability viability. This review synthesizes current on NGP stability, challenges delivery, their applications preventing treating chronic diseases such diabetes, obesity, cardiovascular diseases. We explore physiological characteristics, safety profiles, action various strains while also addressing opportunities presented by integration into clinical practice. NGPs revolutionize microbiome-based therapies improve outcomes is immense, underscoring need further optimize ensure safety.

Язык: Английский

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Potential therapeutic solution for Clostridioides difficile infection: current scenario and future prospects

Medicine in Microecology, Год журнала: 2025, Номер unknown, С. 100121 - 100121

Опубликована: Фев. 1, 2025

Язык: Английский

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Editorial: Omics technologies and bioinformatic tools in probiotic research

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Март 4, 2025

Probiotics are defined as live microorganisms that can promote intestinal and extraintestinal health benefits when consumed in sufficient quantity (Hill et al., 2014). Several bifidobacteria, lactobacilli enterococci have been classified probiotics due to their safety profile health-promoting properties. These commonly found various habitats, including dairy non-dairy fermented products, the mammalian gastrointestinal microbiota environment. For a new strain be probiotic, number of criteria should fulfilled: resistance transit, lack virulence transmissible antibiotic genes, activities (e.g. antimicrobial, immunomodulatory, antioxidant). Regulatory authorities established conventional microbiological assays assess these phenotypes (FAO/WHO, 2001). In addition, high-throughput multi-omics approaches now being applied complement existing methodologies provide deeper molecular cellular insights into probiotic-host interactions (Kiousi 2021).In era (meta)genomics, availability whole genome sequences (WGS) probiotic strains has increased exponentially. The integration genomic element studies supports prediction functional strain. WGS is "gold standard" for taxonomic classification isolates species its higher discriminatory power. Indeed, facilitated reclassification diverse emended Lactobacillus spp. 25 genera based on shared ecological metabolic properties (Zheng 2020). Currently, EFSA requires used food chain monitor genes concern factors, genes) (EFSA, 2024). this context, analyses supplemented with vitro were performed by Wei al. evaluate traits Limosilactobacillus reuteri A51, previously isolated from yak yoghurt. It was codes related stress responses, survival attachment tract, biosynthetic clusters antimicrobial compounds exopolysaccharides. also exhibited tolerance simulated conditions, well antioxidant activity. Wang al.performed similar analysis Lactiplantibacillus plantarum HOM2217, human milk, examined potential an alternative treatment obesity. shown possesses cholesterol removal activity vitro. it could regulate lipid metabolism inflammation, therefore contributing prevention obesity rats fed high-fat diet (HFD). Similarly, Enterococcus rotai CMTB-CA6, medicinal plant Cantella asiatica Kim limited growth skin pathogens, induced commensal dermal fibroblast viability No transferable or factors identified strain, suggesting safe use care products.Apart derived aerobic microaerophilic environments, strictly anaerobic microorganisms, most notably Feacalibacterium prausnitzii Akkermansia muciniphila next-generation (O'Toole 2017).These adapted grow GI tract host thus present evolutionary advantage niche. Vergalito showed A. ATCC BAA-835 conditions adhesion capacity compared Lacticaseibacillus rhamnosus GG, one well-studied strains. Genes mucus-degrading enzymes two mucus-binding proteins involved detected strain.Comparative supported identification conserved antiphage responses bacteria. One adaptive systems clustered regularly interspaced short palindromic repeats (CRISPR) -and CRISPR-associated (Cas) loci encodes primitive acquired immunity system patrols cytoplasm degrades phage-derived (Makarova context industry, phages decrease cell cause lysis, significantly affect fermentation microbial strains, texture aroma end-product (Ranveer CRISPR-Cas arrays distributed strain-specific manner. Rostampour Cas 22% available genomes Lp. subtype II-A common among followed type I-E. Further play more active role defensive larger repeat-spacer arrays. Of note, spacers seem confer plasmid-mediated gene transfer, presenting broader noted engineering leading method efficient manipulation sequences. Therefore, understanding prevalence support expansion bioengineering toolkit (Parvin Sadras, Furthermore, comparative genomics utilized resistance. Aborode identify Escherichia coli genomes. structure products predicted silico docking experiments phytochemicals effective inhibitors. Utilizing approach, authors confirmed hesperidin, flavanone glycoside citrus fruit, presented favorable pharmacokinetics, good stability plausible binding positions against resistant E. carrying MacB, gidB katG genes.In conclusion, Research Topic provides valuable knowledge information mechanisms action novel different sources, such environmental samples. Clearly, field moving towards mechanistic establish causative relationships between administration health. databases, search engines, bioinformatics pipelines developed systematically collect bulk data studies, meaningful interpretation visualization, facilitate selection promising further research. example, Probio-ichnos manually curated, literaturederived database collects presents (Tsifintaris 2024), MASI catalogues substances (Zeng 2021), ODRAP explores prebiotic (Guseva Moreover, machine learning platform iProbiotics rapid (Sun 2022), whereas ProbioMinServer integrated evaluating functionality (Liu 2023). platforms, bioinformatic tools, combination holistic view interplay probiotics, microbiota, tailor-made, strain-, host-, disease-specific supplements.

Язык: Английский

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Co-microencapsulation of Lactobacillus paracasei and Inonotus obliquus polysaccharide in alginate system: physicochemical and functional properties

Food Hydrocolloids, Год журнала: 2025, Номер unknown, С. 111344 - 111344

Опубликована: Март 1, 2025

Язык: Английский

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Diet, microbiome, and probiotics establish a crucial link in vaccine efficacy

Critical Reviews in Microbiology, Год журнала: 2025, Номер unknown, С. 1 - 26

Опубликована: Март 20, 2025

Vaccination plays a critical role in public health by reducing the incidence and prevalence of infectious diseases. The efficacy vaccine has numerous determinants, which include age, sex, genetics, environment, geographic location, nutritional status, maternal antibodies, prior exposure to pathogens. However, little is known about gut microbiome how it can be targeted through dietary interventions improve immunological responses. Unveiling this link imperative, particularly post-pandemic world, considering impaired COVID-19 response observed dysbiotic individuals. Therefore, article aims comprehensively review diet probiotics modulate composition, linked efficacy. Dietary fiber polyphenolic compounds derived from plant-based foods microbial diversity promoting growth short-chain fatty acids-producing microbes. On other hand, animal-based have mixed effects – whey protein fish oil promote eubiosis In contrast, lard red meat adverse effects. Studies further indicate that probiotic supplements exert varied effects, mostly strain dosage-specific. Interlinking diet, microbiome, probiotics, vaccines will reveal opportunities for newer research on diet-induced microbiome-manipulated precision vaccination strategies against

Язык: Английский

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Research progress regarding physiological functions of probiotics and techniques to enhance their stress resistance: a review

Critical Reviews in Food Science and Nutrition, Год журнала: 2025, Номер unknown, С. 1 - 29

Опубликована: Май 5, 2025

Probiotics have received widespread attention for their unique physiological functions, such as promoting digestion and absorption of nutrients, modulating the immune system, inhibiting pathogenic microorganisms. However, probiotics face severe challenges in terms stability viability practical applications, environmental factors like acid, osmotic pressure, temperature, oxidation bile salts, which make it difficult to maintain sufficient numbers when they are delivered target location. Therefore, some technical protection is needed improve adhesion gastrointestinal tract minimize damage probiotic products during processing, transportation handling. We review underlying mechanisms by exert well various strategies enhance tolerance, especially traditional production methods modification genetic pathways. In addition, application tolerogenic fermented foods clinical medicine discussed. Through in-depth analysis potential function tolerance enhancement, not only provide theoretical support probiotics, but also pave way development functional innovative human disease treatment strategies.

Язык: Английский

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Lactiplantibacillus plantarum and Lactiplantibacillus pentosus species isolated from fermented beet root and dragon fruit exhibit potent antibacterial and antibiofilm activity

Biologia, Год журнала: 2025, Номер unknown

Опубликована: Май 20, 2025

Язык: Английский

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Beyond digestion: Exploring how the gut microbiota modulates human social behaviors

Neuroscience, Год журнала: 2024, Номер 565, С. 52 - 62

Опубликована: Ноя. 29, 2024

Язык: Английский

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Perspectives on Microbiome Therapeutics in Infectious Diseases: A Comprehensive Approach Beyond Immunology and Microbiology

Cells, Год журнала: 2024, Номер 13(23), С. 2003 - 2003

Опубликована: Дек. 4, 2024

Although global life expectancy has increased over the past 20 years due to advancements in managing infectious diseases, one-fifth of people still die from infections. In response this ongoing threat, significant efforts are underway develop vaccines and antimicrobial agents. However, pathogens evolve resistance mechanisms, complicating their control. The COVID-19 pandemic underscored limitations focusing solely on pathogen-killing strategies immunology microbiology address complex, multisystemic diseases. This highlights urgent need for practical advancements, such as microbiome therapeutics, that these while complementing traditional approaches. Our review emphasizes key outcomes field, including evidence probiotics reducing disease severity insights into host-microbiome crosstalk have informed novel therapeutic strategies. These findings underscore potential microbiome-based interventions promote physiological function alongside existing aimed at enhancing host immune responses pathogen destruction. narrative explores therapeutics next-generation treatments application role interactions. While offering a perspective grounded cooperative defense system, also addresses challenges translating clinical settings.

Язык: Английский

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Genome mining approach reveals the CRISPR-Cas characteristics and diversity of targeting phages in Lactobacillus iners strains

Gene Reports, Год журнала: 2024, Номер 36, С. 101968 - 101968

Опубликована: Июль 3, 2024

Язык: Английский

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