Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Апрель 10, 2025
Numerous
studies
demonstrate
that
intestinal
microbiota
contribute
to
colorectal
cancer
(CRC),
which
is
often
associated
with
dysbiosis.
Most
of
the
data
were
obtained
from
on
CRC
patients,
making
it
challenging
determine
whether
alterations
in
are
a
consequence
pathology
or
they
actively
drive
its
progression.
Several
using
laboratory
animals
suggest
gut
may
be
involved
both
onset
and
progression
CRC.
In
present
study
we
utilized
azoxymethane-dextran
sulfate
sodium
(AOM/DSS)
mouse
model
investigate
contribution
healthy-state
inflammation-associated
tumorigenesis.
Two
cohorts
C57BL/6
mice
harboring
different
demonstrated
susceptibility
AOM/DSS
treatment.
Sequencing
16S
rRNA
bacterial
DNA
fecal
samples
revealed
Akkermansia
muciniphila
Bacteroides
acidifaciens
as
marker
features
(before
administration),
showed
strong
positive
correlation
tumor
incidence.
Moreover,
abundance
these
markers,
considered
beneficial
bacteria,
was
strongly
positively
correlated
sulfate-reducing
bacteria
Desulfovibrio
fairfieldensis
identified
chronic
colitis-associated
microbiota.
Furthermore,
abundances
features,
outcome,
expression
interferon
gamma
nitric
oxide
synthase
2
genes
colon
tissue
during
early
stage
DSS-induced
inflammation.
contrast
multiple
demonstrating
anti-inflammatory
properties
A.
B.
acidifaciens,
our
results
point
out
their
potential
adverse
effect
under
specific
conditions
genotoxicity
inflammation
intestine.
Taken
together,
findings
complex,
context-dependent
role
commensal
dysbiosis
Future Microbiology,
Год журнала:
2025,
Номер
unknown, С. 1 - 12
Опубликована: Апрель 14, 2025
In
recent
years,
the
study
of
probiotics
has
advanced
significantly,
driven
by
growing
interest
in
their
potential
health
benefits
and
applications
food
pharmaceutical
industries.
Probiotics
are
claimed
to
enhance
gut
health,
modulate
immune
responses,
improve
digestion,
synthesize
beneficial
compounds
for
host,
even
impact
mental
through
gut-brain
axis.
However,
traditional
vitro
methods
identifying
have
limitations,
such
as
low
reproducibility
phenotypic
screening,
limited
capacity
discover
new
strains,
restricted
evaluation
safety,
inefficiencies
fully
understanding
biological
properties
responsible
health-promoting
effects.
Advancements
genomic
analysis
technology
provided
a
cost-effective
approach
further
explore
probiotic
strains
molecular
mechanisms
driving
effects
hosts.
Here,
we
describe
comprehensive
workflow
probiogenomic
aimed
at
establishing
gold-standard
pipeline
screening
based
on
genome
sequencing.
This
encompasses
steps
from
acquiring
genomes
safety-related
features,
plasticity,
markers
whole-genome
addition,
this
outlines
respective
methodological
approaches
provides
most
database
documented
date,
comprising
243
genes
potentially
associated
with
function.
Nutrients,
Год журнала:
2024,
Номер
16(21), С. 3757 - 3757
Опубликована: Окт. 31, 2024
Bisphenols,
such
as
bisphenol
A
and
its
analogs,
which
include
S,
F,
AF,
tetramethyl
are
chemical
contaminants
commonly
found
in
food
that
raise
serious
health
concerns.
These
xenobiotics
can
potentially
have
harmful
effects
on
human
health.
The
gut
microbiota
plays
a
crucial
role
metabolizing
neutralizing
these
substances,
is
essential
for
their
detoxification
elimination.
Probiotic
supplementation
has
been
studied
ability
to
modulate
the
microbiota's
composition
function,
enhancing
processes.
Next-Generation
Probiotics
(NGPs)
may
exhibit
better
properties
than
traditional
strains
designed
targeted
action
specific
conditions,
obesity.
By
modulating
inflammatory
responses
reducing
secretion
of
pro-inflammatory
cytokines,
they
significantly
improve
host
Research
NGPs'
neutralize
obesogenic
bisphenols
remains
limited,
but
potential
makes
this
promising
area
future
exploration.
This
review
aims
understand
mechanisms
transformation
through
interactions
with
probiotics,
particularly
NGPs,
Understanding
interplay
between
bisphenols,
microbiota,
NGPs
pave
way
strategies
counteract
negative
associated
daily
chronic
exposure
safety
consumer
protection.
Journal of Translational Autoimmunity,
Год журнала:
2025,
Номер
10, С. 100269 - 100269
Опубликована: Янв. 8, 2025
Autoimmune
rheumatic
diseases
(ARDs)
are
a
heterogeneous
group
of
conditions
characterized
by
excessive
and
misdirected
immune
responses
against
the
body's
own
musculoskeletal
tissues.
Their
exact
aetiology
remains
unclear,
with
genetic,
demographic,
behavioural
environmental
factors
implicated
in
disease
onset.
One
prominent
hypothesis
for
initial
breach
tolerance
(leading
to
autoimmunity)
is
molecular
mimicry,
which
describes
structural
or
sequence
similarities
between
human
microbial
proteins
(mimotopes).
This
similarity
can
lead
cross-reactive
antibodies
T-cell
receptors,
resulting
an
response
autoantigens.
Both
commensal
microbes
microbiome
pathogens
trigger
thereby
potentially
contributing
onset
ARDs.
In
this
review,
we
focus
on
role
mimicry
rheumatoid
arthritis
systemic
lupus
erythematosus.
Moreover,
implications
also
briefly
discussed
ankylosing
spondylitis,
sclerosis
myositis.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(3), С. 1058 - 1058
Опубликована: Янв. 26, 2025
Probiotic
bacteria
are
normal
inhabitants
of
a
healthy
human
gut,
conferring
multiple
beneficial
effects
on
the
gut
and
beyond.
Under
various
disease
states,
abundance
diversity
significantly
decreased,
process
called
dysbiosis.
Among
intra-
extracellular
components
probiotics,
vesicles
(EVs)
secreted
by
them
have
recently
garnered
significant
attention
as
potential
mediators
probiotics’
host
health.
Further,
these
nanosized
particles
that
encapsulate
wide
range
bioactive
molecules
(proteins,
lipids,
RNA,
DNA)
standing
out
key
factors
could
mediate
microbiota–host
communication
confer
ameliorating
in
experimental
inflammatory,
metabolic,
cardiovascular
models.
However,
standard
protocol
EV
isolation
from
probiotic
bacteria,
not
varying
lab
to
lab,
must
be
established
achieve
consistency
results
pre-clinical
Our
current
study
compared
two
commonly
used
methods
for
biological
samples,
ultracentrifugation
precipitation,
develop
isolating
EVs
probiotics
Lactobacillus
acidophilus
(LA),
Gram-positive
bacterium,
Escherichia
coli
Nissle
(EcN),
Gram-negative
bacterium.
The
method
gave
~1.5-fold
higher
yield
both
LA
EcN
precipitation
method.
released
level
LA.
were
quantified
characterized
nanoparticle-tracking
analysis
(NTA)
measuring
specific
surface
biomarkers
using
Western
blot.
Here,
we
describe
our
standardized
step-by-step
their
characterization.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(3), С. 1119 - 1119
Опубликована: Янв. 28, 2025
Finding
and
characterizing
new
bacterial
strains,
including
probiotic
is
a
crucial
task
in
today’s
world
to
expand
the
genetic
data
pool
identify
genes.
In
this
study,
we
investigated
gut
microbiota
of
one
industrial
species,
Cyprinus
carpio,
identified
representatives
various
microbial
genera,
Citrobacter,
Serratia,
Bacillus,
Enterococcus,
Kocuria.
Notably,
discovered
two
strains
Hafnia
with
potentially
properties.
We
conducted
next-generation
sequencing
(NGS)
these
described
their
antibiotic
resistance
antibacterial
activity,
compared
them
other
genus.
These
characterized
by
rapid
growth,
presence
ClpB
heat
shock
protein
gene,
genes
associated
microplastic
degradation,
provide
promising
basis
for
further
research,
studies
on
potential
application
plastic
biodegradation.
Pharmacological Research,
Год журнала:
2025,
Номер
unknown, С. 107672 - 107672
Опубликована: Фев. 1, 2025
The
characteristic
of
cardiorenal
syndrome
(CRS)
is
simultaneous
damage
to
both
the
heart
and
kidneys.
CRS
has
caused
a
heavy
burden
mortality
incidence
rates
worldwide.
regulation
host
microbiota
metabolism
that
triggers
kidney
an
emerging
research
field
promotes
new
perspective
on
cardiovascular
risk.
We
summarize
current
studies
from
bench
bedside
gut
microbiota-derived
metabolites
better
understand
in
context
metabolites.
focused
involvement
pathophysiology
CRS,
including
lipid
cholesterol
disorders,
coagulation
abnormalities
platelet
aggregation,
oxidative
stress,
endothelial
dysfunction,
inflammation,
mitochondrial
energy
vascular
calcification
renal
fibrosis,
as
well
therapeutic
approaches
targeting
which
provides
innovative
treatment
approach
for
improve
patient
prognosis
overall
quality
life.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Март 12, 2025
Background
Mild
intestinal
dysfunction,
linked
to
subtle
yet
significant
health
issues,
can
be
induced
by
lipopolysaccharide
(LPS),
a
Gram-negative
bacterial
component
that
disrupts
gut
function
and
triggers
inflammation.
Akkermansia
muciniphila
has
shown
promise
as
probiotic
for
due
its
roles
in
mucin
degradation
short-chain
fatty
acid
production.
This
study
explores
the
therapeutic
effects
of
on
LPS-induced
mild
dysfunction
mice.
Methods
Thirty-eight
6-week-old
C57BL/6
mice
were
split
into
control
(
n
=
19)
LPS-treated
groups.
received
300
μg/kg/day
LPS
4
weeks,
followed
supplementation
at
41
mg/kg/day
(Akk1)
or
82
(Akk2)
another
weeks.
Gut
microbiota
was
analyzed
via
metagenomic
sequencing,
gene
expression
evaluated
through
transcriptomics.
Results
significantly
altered
microbiota,
reducing
diversity
increasing
pathogenic
genera
like
Lachnoclostridium.
supplementation,
particularly
higher
doses,
partially
restored
beneficial
such
Muribaculum.
Transcriptomics
showed
immune
inflammatory
responses,
while
reduced
these
modulating
pathways
TNF
NF-kappa
B
signaling.
Conclusion
mitigates
restoring
balance
highlighting
potential
agent
health.
