TNMD in pan-cancer analysis: Exploring its impact on immune modulation and uncovering functional insights in colorectal cancer DOI Creative Commons
Jingfeng Chen,

Qin Hu,

Cong Zhang

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 4, 2024

Abstract Background Tendomodulin (TNMD) is pivotal in various malignancies, including colorectal cancer (CRC). However, its comprehensive impact across cancers, particularly immunomodulatory function CRC, remains underexplored. This study explored the role of TNMD CRC by focusing on functions through molecular and clinical analyses. Methods Multiple bioinformatics databases analytical tools were utilized for TNMD pan-cancer analysis. To validate we performed experiments, immunofluorescence (IF), immunohistochemistry (IHC), real-time quantitative reverse transcription PCR (qPCR), Western blotting, cell migration assays. Results expression gene mutation vary cancers offer high diagnostic value. Survival analysis has found that associated with prognosis multiple cancers. Notably, patients microsatellite instability (MSI-H) correlated positively immune cells, natural killer (NK) whereas it was inversely regulatory T cells (Tregs). Crucially, stability (MSS) better immunotherapy outcomes, indicating potential as a biomarker patient stratification tailored treatment approaches. Furthermore, single-cell sequencing data revealed stronger interactions between TNMD-positive tumor fibroblasts or macrophages microenvironment. Finally, overexpressed tissues lines, thereby promoting invasion metastasis. Conclusions Our findings reveal critical influencing tumor–immune interactions. Beyond prognostic biomarker, promotes metastasis invasion, thus emerging promising therapeutic target. These highlight TNMD's significance potentially other malignancies.

Язык: Английский

Tendomodulin in pan-cancer analysis: exploring its impact on immune modulation and uncovering functional insights in colorectal cancer DOI Creative Commons
Jingfeng Chen,

Qin Hu,

Cong Zhang

и другие.

BMC Cancer, Год журнала: 2025, Номер 25(1)

Опубликована: Фев. 11, 2025

Tendomodulin (TNMD) is pivotal in various malignancies, including colorectal cancer (CRC). However, its comprehensive impact across cancers, particularly immunomodulatory function CRC, remains underexplored. This study explored the role of TNMD CRC by focusing on functions through molecular and clinical analyses. Multiple bioinformatics databases analytical tools were utilized for pan-cancer analysis. To validate we performed experiments, immunofluorescence (IF), immunohistochemistry (IHC), real-time quantitative reverse transcription PCR (qPCR), western blotting, cell migration assays. expression gene mutation vary cancers offer high diagnostic value. Survival analysis found that associated with prognosis multiple cancers. Notably, patients microsatellite instability (MSI-H) correlated positively immune cells, natural killer (NK) whereas it was inversely regulatory T cells (Tregs). Crucially, stability (MSS) better immunotherapy outcomes, indicating potential as a biomarker patient stratification tailored treatment approaches. Furthermore, single-cell sequencing data revealed stronger interactions between TNMD-positive tumor fibroblasts or macrophages microenvironment. Finally, overexpressed tissues lines, thereby promoting invasion metastasis. Our findings reveal critical influencing tumor–immune interactions. Beyond prognostic biomarker, promotes metastasis invasion, thus emerging promising therapeutic target. These highlight TNMD's significance potentially other malignancies.

Язык: Английский

Процитировано

0
The reciprocal effects of autophagy and the Warburg effect in pancreatic ductal adenocarcinoma: an in vitro study DOI

Bita Azizzadeh,

Maryam Majidinia, Ali Gheysarzadeh

и другие.

Medical Oncology, Год журнала: 2025, Номер 42(4)

Опубликована: Фев. 28, 2025

Язык: Английский

Процитировано

0
Overcoming Resistance of Caco-2 Cells to 5-Fluorouracil through Diruthenium Complex Encapsulation in PMMA Nanoparticles DOI Creative Commons
Isabel Coloma, Jorge Parrón‐Ballesteros, Miguel Cortijo

и другие.

Inorganic Chemistry, Год журнала: 2024, Номер 63(28), С. 12870 - 12879

Опубликована: Июнь 4, 2024

Drug resistance, one of the main drawbacks in cancer chemotherapy, can be tackled by employing a combination drugs that target different biological processes cell, enhancing therapeutic efficacy. Herein, we report synthesis and characterization new paddlewheel diruthenium complex includes 5-fluorouracil (5-FU), commonly used anticancer drug. This drug was functionalized with carboxylate group to take advantage previously demonstrated release capacity ligands from core. The resulting hydrophobic complex, [Ru2Cl(DPhF)3(5-FUA)] (Ru-5-FUA) (DPhF = N,N′-diphenylformamidinate; 5-FUA 5-fluorouracil-1-acetate) subsequently entrapped poly(methyl methacrylate) (PMMA) nanoparticles (PMMA@Ru-5-FUA) via reprecipitation method transported media. optimized encapsulation procedure yielded particles an average size 81.2 nm, PDI 0.11, zeta potential 29.2 mV. cytotoxicity tested vitro using human colon carcinoma cell line Caco-2. IC50 (half maximal inhibitory concentration) PMMA@Ru-5-FUA (6.08 μM) just slightly lower than found for 5-FU (7.64 μM). Most importantly, while cells seemed have developed resistance against 5-FU, showed almost complete lethality at ∼30 μM. Conversely, analogous devoid moiety, [Ru2Cl(DPhF)3(O2CCH3)] (PMMA@RuA), displayed reduced equivalent concentrations. These findings highlight effect combining properties those species. suggests distinct modes action two chemical species are crucial overcoming resistance.

Язык: Английский

Процитировано

3
TNMD in pan-cancer analysis: Exploring its impact on immune modulation and uncovering functional insights in colorectal cancer DOI Creative Commons
Jingfeng Chen,

Qin Hu,

Cong Zhang

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 4, 2024

Abstract Background Tendomodulin (TNMD) is pivotal in various malignancies, including colorectal cancer (CRC). However, its comprehensive impact across cancers, particularly immunomodulatory function CRC, remains underexplored. This study explored the role of TNMD CRC by focusing on functions through molecular and clinical analyses. Methods Multiple bioinformatics databases analytical tools were utilized for TNMD pan-cancer analysis. To validate we performed experiments, immunofluorescence (IF), immunohistochemistry (IHC), real-time quantitative reverse transcription PCR (qPCR), Western blotting, cell migration assays. Results expression gene mutation vary cancers offer high diagnostic value. Survival analysis has found that associated with prognosis multiple cancers. Notably, patients microsatellite instability (MSI-H) correlated positively immune cells, natural killer (NK) whereas it was inversely regulatory T cells (Tregs). Crucially, stability (MSS) better immunotherapy outcomes, indicating potential as a biomarker patient stratification tailored treatment approaches. Furthermore, single-cell sequencing data revealed stronger interactions between TNMD-positive tumor fibroblasts or macrophages microenvironment. Finally, overexpressed tissues lines, thereby promoting invasion metastasis. Conclusions Our findings reveal critical influencing tumor–immune interactions. Beyond prognostic biomarker, promotes metastasis invasion, thus emerging promising therapeutic target. These highlight TNMD's significance potentially other malignancies.

Язык: Английский

Процитировано

0