Research Progress and Application of Miniature CRISPR-Cas12 System in Gene Editing DOI Open Access

Qiangbing Xuan,

Junjie Wang, Yingbin Nie

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12686 - 12686

Опубликована: Ноя. 26, 2024

CRISPR-Cas system, a natural acquired immune system in prokaryotes that defends against exogenous DNA invasion because of its simple structure and easy operation, has been widely used many research fields such as synthetic biology, crop genetics breeding, precision medicine, so on. The miniature CRISPR-Cas12 an emerging genome editing tool recent years. Compared to the commonly CRISPR-Cas9 CRISPR-Cas12a, unique advantages, rich PAM sites, higher specificity, smaller volume, cytotoxicity. However, application Cas12 proteins methods improve efficiency have not systematically summarized. In this review, we introduce classification summarize structural characteristics type V cleavage mechanism five proteins. gene animals, plants, microorganisms is summarized, strategies are discussed, aiming provide reference for further understanding functional engineering modification system.

Язык: Английский

Natural Products and Health Care Functions of Inonotus obliquus DOI Creative Commons

Y.F. Wang,

Jingsheng Gu,

Jiaying Wu

и другие.

Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(4), С. 269 - 269

Опубликована: Апрель 10, 2025

With the increasing attention of modern medicine to natural medicinal agents, Inonotus obliquus (chaga), a macrofungus with remarkable value, has gradually garnered widespread academic interest. This paper reviews primary bioactive components I. in recent years, including polysaccharides, phenolic compounds, and triterpenoids, which exhibit diverse pharmacological effects such as antioxidant, anti-inflammatory, immunomodulatory, antitumor activities. It further discusses how these enhance human health disease resistance through distinct biological mechanisms, activation antioxidant systems, regulation immune responses, modulation apoptosis pathways. Additionally, article explores biosynthetic pathways metabolites their relevance. Finally, we summarize potential resource envisage its future applications clinical drug development. review aims provide novel perspectives for cultivation, utilization, industrial-scale exploitation obliquus.

Язык: Английский

Процитировано

0

Research Progress and Application of Miniature CRISPR-Cas12 System in Gene Editing DOI Open Access

Qiangbing Xuan,

Junjie Wang, Yingbin Nie

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12686 - 12686

Опубликована: Ноя. 26, 2024

CRISPR-Cas system, a natural acquired immune system in prokaryotes that defends against exogenous DNA invasion because of its simple structure and easy operation, has been widely used many research fields such as synthetic biology, crop genetics breeding, precision medicine, so on. The miniature CRISPR-Cas12 an emerging genome editing tool recent years. Compared to the commonly CRISPR-Cas9 CRISPR-Cas12a, unique advantages, rich PAM sites, higher specificity, smaller volume, cytotoxicity. However, application Cas12 proteins methods improve efficiency have not systematically summarized. In this review, we introduce classification summarize structural characteristics type V cleavage mechanism five proteins. gene animals, plants, microorganisms is summarized, strategies are discussed, aiming provide reference for further understanding functional engineering modification system.

Язык: Английский

Процитировано

1