Therapeatic evaluation and single cell analysis of adipose stromal vascular fraction isolation from a commercial cell separation system
Abstract
Background:
In
the
field
of
regenerative
therapy,
stromal
vascular
fraction
(SVF)
extracted
from
adipose
tissue
has
been
widely
recognized
for
its
significant
benefits.
However,
cellular
composition
and
therapeutic
effect
SVF
products
prepared
via
different
methods
are
unclear.
Methods:
cells
were
obtained
three
approaches:
(1)
generation
mechanical
emulsification
(M-SVF),
(2)
laboratory
enzymatic
digestion
(L-SVF),
(3)
commercial
cell
separation
systems
(C-SVF).
We
evaluated
their
healing
effects
on
mouse
wounds.
Additionally,
we
utilized
single-nucleus
RNA
sequencing
(snRNA-seq)
technology
to
explore
C-SVF.
Results:
The
yield
C-SVF
was
comparable
that
L-SVF.
During
in
vitro
culture,
exhibited
enhanced
proliferation
a
reduced
proportion
apoptotic
cells.
In
wound
model,
application
facilitated
closure
wounds
improved
collagen
remodeling
angiogenesis
area.
Additional
snRNA-seq
analysis
revealed
APOE+
adipose-derived
stem
immune
cells,
especially
M2
anti-inflammatory
macrophages,
enriched
C-SVF,
which
together
promote
repair,
(ADSCs)
jointly
regulate
repair.
Conclusion:
A
extraction
system
is
an
effective
method
isolating
viable
with
ADSCs
macrophages.
Research Square (Research Square), Год журнала: 2025, Номер unknown
Опубликована: Май 16, 2025
Язык: Английский
