Therapeatic evaluation and single cell analysis of adipose stromal vascular fraction isolation from a commercial cell separation system DOI

Shunxin Han,

Qian Zhang, Feng Lu

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Май 16, 2025

Abstract Background: In the field of regenerative therapy, stromal vascular fraction (SVF) extracted from adipose tissue has been widely recognized for its significant benefits. However, cellular composition and therapeutic effect SVF products prepared via different methods are unclear. Methods: cells were obtained three approaches: (1) generation mechanical emulsification (M-SVF), (2) laboratory enzymatic digestion (L-SVF), (3) commercial cell separation systems (C-SVF). We evaluated their healing effects on mouse wounds. Additionally, we utilized single-nucleus RNA sequencing (snRNA-seq) technology to explore C-SVF. Results: The yield C-SVF was comparable that L-SVF. During in vitro culture, exhibited enhanced proliferation a reduced proportion apoptotic cells. In wound model, application facilitated closure wounds improved collagen remodeling angiogenesis area. Additional snRNA-seq analysis revealed APOE+ adipose-derived stem immune cells, especially M2 anti-inflammatory macrophages, enriched C-SVF, which together promote repair, (ADSCs) jointly regulate repair. Conclusion: A extraction system is an effective method isolating viable with ADSCs macrophages.

Язык: Английский

Silencing of long non-coding RNAs MIR22HG, LNCTAM34A, and TP53TG1 triggers Cell survival/proliferation and inhibits apoptosis in women’s breast cancer DOI Creative Commons

Ahmed Al-Kateb,

Roozbeh Heidarzadehpilehrood, Maryam Pirhoushiaran

и другие.

Advances in Cancer Biology - Metastasis, Год журнала: 2025, Номер unknown, С. 100133 - 100133

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0

Therapeatic evaluation and single cell analysis of adipose stromal vascular fraction isolation from a commercial cell separation system DOI

Shunxin Han,

Qian Zhang, Feng Lu

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Май 16, 2025

Abstract Background: In the field of regenerative therapy, stromal vascular fraction (SVF) extracted from adipose tissue has been widely recognized for its significant benefits. However, cellular composition and therapeutic effect SVF products prepared via different methods are unclear. Methods: cells were obtained three approaches: (1) generation mechanical emulsification (M-SVF), (2) laboratory enzymatic digestion (L-SVF), (3) commercial cell separation systems (C-SVF). We evaluated their healing effects on mouse wounds. Additionally, we utilized single-nucleus RNA sequencing (snRNA-seq) technology to explore C-SVF. Results: The yield C-SVF was comparable that L-SVF. During in vitro culture, exhibited enhanced proliferation a reduced proportion apoptotic cells. In wound model, application facilitated closure wounds improved collagen remodeling angiogenesis area. Additional snRNA-seq analysis revealed APOE+ adipose-derived stem immune cells, especially M2 anti-inflammatory macrophages, enriched C-SVF, which together promote repair, (ADSCs) jointly regulate repair. Conclusion: A extraction system is an effective method isolating viable with ADSCs macrophages.

Язык: Английский

Процитировано

0