Frontier progress and translational challenges of pluripotent differentiation of stem cells DOI Creative Commons

Zhengbing Su,

Hui Dong, Xiang Fang

и другие.

Frontiers in Genetics, Год журнала: 2025, Номер 16

Опубликована: Апрель 28, 2025

Stem cell research has significantly transformed regenerative medicine, with pluripotent stem cells (PSCs) serving as the cornerstone for disease modeling, drug screening, and therapeutic applications. Embryonic (ESCs) exhibit unparalleled self-renewal tri-lineage differentiation, while induced (iPSCs) bypass ethical constraints through somatic reprogramming. Clinical trials highlight potential of mesenchymal (MSCs) in osteoarthritis graft-versus-host disease, which leverage their immunomodulatory paracrine effects. Despite advancements, challenges persist: iPSCs face epigenetic instability tumorigenic risks, adult struggle inefficient differentiation. This paper systematically reviews source classification, differentiation regulatory mechanisms, cutting-edge technologies such CRISPR/Cas9, explores field-specific controversies (e.g., stability iPSCs) future directions integration organoids biomaterials). By analyzing current progress challenges, it provides a multidimensional perspective research.

Язык: Английский

Frontier progress and translational challenges of pluripotent differentiation of stem cells DOI Creative Commons

Zhengbing Su,

Hui Dong, Xiang Fang

и другие.

Frontiers in Genetics, Год журнала: 2025, Номер 16

Опубликована: Апрель 28, 2025

Stem cell research has significantly transformed regenerative medicine, with pluripotent stem cells (PSCs) serving as the cornerstone for disease modeling, drug screening, and therapeutic applications. Embryonic (ESCs) exhibit unparalleled self-renewal tri-lineage differentiation, while induced (iPSCs) bypass ethical constraints through somatic reprogramming. Clinical trials highlight potential of mesenchymal (MSCs) in osteoarthritis graft-versus-host disease, which leverage their immunomodulatory paracrine effects. Despite advancements, challenges persist: iPSCs face epigenetic instability tumorigenic risks, adult struggle inefficient differentiation. This paper systematically reviews source classification, differentiation regulatory mechanisms, cutting-edge technologies such CRISPR/Cas9, explores field-specific controversies (e.g., stability iPSCs) future directions integration organoids biomaterials). By analyzing current progress challenges, it provides a multidimensional perspective research.

Язык: Английский

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