Genome-Wide Identification of the CIF Gene Family and Protein Interaction with GSO1s Under the p-HBA-Induced Continuous Cropping Obstacle in Pogostemon cablin

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1568 - 1568

Опубликована: Фев. 13, 2025

Casparian strip integrity factors (CIFs), which are tyrosine-sulfated small peptides, crucial genes involved in the formation and regulation of play an important role plant stress response. In order to explore evolution, characteristics, role, function CIFs response continuous cropping obstacles (CCOs), bioinformatics gene expression analysis CIF Pogostemon cablin was carried out by determining phylogenetic relationship, chromosome location, structure, RT-qPCR results. Results showed that a total 12 PatCIF family were identified on different chromosomes. Promoter prediction revealed 16 cis-regulatory elements. A systematic evolutionary study 33 species indicates originated from Spermatophyta. Collinearity P. shared 19 syntenic with Solanum lycopersicum only 8 Oryza sativa. Transcriptome indicated PatCIF1-4 PatGSO1b/1c/1f decreased under p-hydroxybenzoic acid treatment, further validation four consistent AlphaFold protein interaction region between mature peptide via LRR domain, provides key binding surface for PatCIFs. This offers theoretical basis investigate roles PatCIFs PatGSO1s CCOs their interactions cablin.

Язык: Английский

Continuous Cropping of Tussilago farfara L. Has a Significant Impact on the Yield and Quality of Its Flower Buds, and Physicochemical Properties and the Microbial Communities of Rhizosphere Soil

Life, Год журнала: 2025, Номер 15(3), С. 404 - 404

Опубликована: Март 4, 2025

Continuous cropping obstacles pose significant constraints and urgent challenges in the production of Tussilago farfara L. This experiment investigated effects consecutive on T. over periods 1, 2, 3 years. It assessed yield quality flower buds, addition to physicochemical properties rhizosphere soil. The microbial community was analyzed through 16S rDNA ITS sequencing using Illumina Novaseq high-throughput technology, while also examining correlations among these factors. results reveal that as duration continuous increases, along with contents extract, tussilagone, total flavonoids, steadily decreased; soil pH, organic matter, available phosphorus, potassium, alkaline nitrogen, activities sucrose, catalase, phosphatase markedly decreased. As quantity diversity bacteria initially increase then decrease, number fungal species increases by 22.5%. Meanwhile, contributes a gradual reduction relative abundance beneficial genera such Ralstonia, Nitrospira, Trichoderma soil, harmful Mortierella, Fusarium, Tricharina accumulate significantly. Correlation analysis shows changes communities notably influence growth quality. study elucidates impacts properties, rhizosphere, providing scientific basis for further research barriers selection farfara.

Язык: Английский

Carbon flow from roots to rhizobacterial networks: Grafting effects

Soil Biology and Biochemistry, Год журнала: 2024, Номер 199, С. 109580 - 109580

Опубликована: Сен. 3, 2024

Язык: Английский