Oxidative Stress-Induced Alteration of Plant Central Metabolism

Life, Год журнала: 2021, Номер 11(4), С. 304 - 304

Опубликована: Апрель 1, 2021

Oxidative stress is an integral component of various conditions in plants, and this fact largely determines the substantial overlap physiological molecular responses to biotic abiotic environmental challenges. In review, we discuss alterations central metabolism occurring plants experiencing oxidative stress. To focus on changes metabolite profile associated with per se, primarily analyzed information generated studies based exogenous application agents, inducing stress, analysis mutants displaying altered response. Despite significant variation among different plant species tissues, dynamic transient character stress-induced metabolites, strong dependence metabolic intensity specific characteristic sugars, sugar derivatives, tricarboxylic acid cycle amino acids, adaptation have been detected. The presented available data demonstrates redistribution fluxes targeted at enhancement tolerance through prevention ROS accumulation, maintenance biosynthesis indispensable production protective compounds. This provides a theoretical basis for selection/generation improved development markers applicable research routine agricultural practice.

Язык: Английский

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Advances in Plant Metabolomics and Its Applications in Stress and Single-Cell Biology

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(13), С. 6985 - 6985

Опубликована: Июнь 23, 2022

In the past two decades, post-genomic era envisaged high-throughput technologies, resulting in more species with available genome sequences. In-depth multi-omics approaches have evolved to integrate cellular processes at various levels into a systems biology knowledge base. Metabolomics plays crucial role molecular networking bridge gaps between genotypes and phenotypes. However, greater complexity of metabolites diverse chemical physical properties has limited advances plant metabolomics. For several years, applications liquid/gas chromatography (LC/GC)-mass spectrometry (MS) nuclear magnetic resonance (NMR) been constantly developed. Recently, ion mobility (IMS)-MS shown utility resolving isomeric isobaric metabolites. Both MS NMR combined metabolomics significantly increased identification quantification an untargeted targeted manner. Thus, hyphenated tools will narrow gap number metabolite features identified Metabolites change response environmental conditions, including biotic abiotic stress factors. The spatial distribution across different organs, tissues, cells compartments is trending research area Herein, we review recent technological advancements their understanding organization. addition, discuss opportunities challenges multiple interactions, multi-omics, single-cell

Язык: Английский

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Differential Physio-Biochemical and Metabolic Responses of Peanut (Arachis hypogaea L.) under Multiple Abiotic Stress Conditions

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(2), С. 660 - 660

Опубликована: Янв. 8, 2022

The frequency and severity of extreme climatic conditions such as drought, salinity, cold, heat are increasing due to climate change. Moreover, in the field, plants affected by multiple abiotic stresses simultaneously or sequentially. Thus, it is imperative compare effects stress combinations on crop relative individual stresses. This study investigated differential regulation physio-biochemical metabolomics parameters peanut (Arachis hypogaea L.) under (salt, heat) combined treatments using multivariate correlation analysis. results showed that heat, salt, drought compounds effect Combined included had highest electrolyte leakage lowest water content. Lipid peroxidation chlorophyll contents did not significantly change Biochemical parameters, free amino acids, polyphenol, starch, sugars, changed compared Free acids increased heat; polyphenols drought; proline concentration salt. Metabolomics data were obtained different can be used identify molecular phenotypes involved acclimation response changing conditions. Peanut identified 160 metabolites, including sugar alcohols, organic fatty other compounds. Pathway enrichment analysis revealed acid, sugar, metabolism. metabolites associated with tricarboxylic acid (TCA) urea cycles biosynthesis pathway intermediates. Principal component (PCA), partial least squares-discriminant (PLS-DA), heatmap potential marker (pinitol, malic xylopyranose) combinations, which could breeding efforts develop cultivars resilient will also facilitate researchers explore indicators resistant for future improvement programs.

Язык: Английский

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When Phased without Water: Biophysics of Cellular Desiccation, from Biomolecules to Condensates

Chemical Reviews, Год журнала: 2023, Номер 123(14), С. 9010 - 9035

Опубликована: Май 3, 2023

The molecular machinery that enables life has evolved in water, yet many of the organisms around us are able to survive even extreme desiccation. Especially remarkable single-cell and sedentary rely on specialized biomolecular environments routinely subjected a near-complete lack water. In this review, we zoom level what is happening cellular environment under water stress. We cover various mechanisms by which biochemical components cell can dysfunction dehydrated cells detail different strategies have eliminate or cope with these desiccation-induced perturbations. specifically focus two survival strategies: (1) use disordered proteins protect before, during, recovery from desiccation, (2) condensates as self-assembly mechanism sequester specific times provide summary experimental work describing critical contributions response loss highlight their role desiccation tolerance. Desiccation biology an exciting area biology, still far being completely explored. Understanding it bound give new insights how adapted/can adapt spanning early colonization land deal climate change our future.

Язык: Английский

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Proline, Cysteine and Branched-Chain Amino Acids in Abiotic Stress Response of Land Plants and Microalgae

Plants, Год журнала: 2023, Номер 12(19), С. 3410 - 3410

Опубликована: Сен. 28, 2023

Proteinogenic amino acids are the building blocks of protein, and plants synthesize all them. In addition to their importance in plant growth development, growing evidence underlines central role played by derivatives regulating several pathways involved biotic abiotic stress responses. present review, we illustrate (i) as an energy source capable replacing sugars electron donors mitochondrial transport chain (ii) precursors osmolytes well (iii) secondary metabolites. Among drought response, proline cysteine play a special role. Besides large accumulation occurring response stress, can export reducing equivalents sink tissues organs, production H2S deriving from metabolism mediate post-translational modifications that target protein cysteines themselves. Although our general understanding microalgae physiology is still fragmentary, overview how unicellular photosynthetic organisms deal with salt also provided because interest applied sciences.

Язык: Английский

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Sulfide promotes tolerance to drought through protein persulfidation in Arabidopsis

Journal of Experimental Botany, Год журнала: 2023, Номер 74(15), С. 4654 - 4669

Опубликована: Май 6, 2023

Abstract Hydrogen sulfide (H2S) is a signaling molecule that regulates essential plant processes. In this study, the role of H2S during drought was analysed, focusing on underlying mechanism. Pretreatments with before imposing plants substantially improved characteristic stressed phenotypes under and decreased levels typical biochemical stress markers such as anthocyanin, proline, hydrogen peroxide. also regulated drought-responsive genes amino acid metabolism, repressed drought-induced bulk autophagy protein ubiquitination, demonstrating protective effects pretreatment. Quantitative proteomic analysis identified 887 significantly different persulfidated proteins between control plants. Bioinformatic analyses more in revealed most enriched biological processes were cellular response to oxidative peroxide catabolism. Protein degradation, abiotic responses, phenylpropanoid pathway highlighted, suggesting importance persulfidation coping stress. Our findings emphasize promoter enhanced tolerance drought, enabling respond rapidly efficiently. Furthermore, main alleviating reactive oxygen species accumulation balancing redox homeostasis highlighted.

Язык: Английский

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OsBCAT2, a gene responsible for the degradation of branched‐chain amino acids, positively regulates salt tolerance by promoting the synthesis of vitamin B5

New Phytologist, Год журнала: 2024, Номер 241(6), С. 2558 - 2574

Опубликована: Янв. 23, 2024

Summary Salt stress negatively affects rice growth, development and yield. Metabolic adjustments contribute to the adaptation of under salt stress. Branched‐chain amino acids (BCAA) are three essential that cannot be synthesized by humans or animals. However, little is known about role BCAA in response plants. Here, we showed BCAAs may function as scavengers reactive oxygen species (ROS) provide protection against damage caused salinity. We determined branched‐chain aminotransferase 2 (OsBCAT2), a protein responsible for degradation BCAA, positively regulates tolerance. significantly induces expression OsBCAT2 rather than synthesis genes, which indicated mainly promotes not de novo synthesis. Metabolomics analysis revealed vitamin B5 (VB5) biosynthesis pathway intermediates were higher ‐overexpressing plants but lower osbcat2 mutants The stress‐sensitive phenotypes rescued exogenous VB5, indicating tolerance regulating VB5 Our work provides new insights into enzymes involved sheds light on molecular mechanism

Язык: Английский

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Microalgal biomass application promotes growth and alleviates salt stress in melon plants stimulating antioxidant metabolism

Journal of Applied Phycology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 23, 2025

Язык: Английский

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Salicylic Acid Improved the Growth of Dunaliella salina and Increased the Proportion of 9-cis-β-Carotene Isomers

Marine Drugs, Год журнала: 2025, Номер 23(1), С. 18 - 18

Опубликована: Янв. 1, 2025

Dunaliella salina is an important source of natural β-carotene (containing 9-cis and all trans isomers) for industrial production. The phytohormone salicylic acid (SA) has been proven to have impacts on the stress resistance higher plants, but research microalgae currently unclear. In this study, effects SA growth, biochemical composition, antioxidant enzyme activity, key enzymes synthesis, cis-and trans-isomers in D. under different salt concentrations were investigated. results shown that at 1.5, 2, 2.5 M NaCl, activity synthesis algal cells significantly increased, content proportion isomer isomers decreased. addition increased growth (SOD, MDA) as well phytoene synthase (PSY), desaturase (PDS), lycopene β cyclase (LCYB) high-salinity conditions. It worth noting treatment SA, three (1.5, NaCl) by 32.09%, 20.30%, 11.32%, respectively. Moreover, can not only improve tolerance salina, also increase isomer, with physiological β-carotene, thereby enhancing application value salina.

Язык: Английский

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Transcriptomic and metabolomic analysis reveal the cold tolerance mechanism of common beans under cold stress

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Март 15, 2025

Common bean (Phaseolus vulgaris L.) is a thermophilic crop, and exposure to cold stress can significantly impact their yield quality. To elucidate the of on cold-tolerant 'Wei Yuan' (WY) cold-sensitive 'Bai Bu Lao' (BBL) common bean, mechanism tolerance was studied by physiological biochemical multi-omics analysis. In this study, lower relative conductivity higher malondialdehyde content after endowed 'WY' seedlings with tolerance. A total 11,837 differentially expressed genes (DEGs) 923 differential metabolites (DEMs) were identified transcriptome metabolomics Joint analysis showed that under stress, DEGs DEMs in beans are extensively engaged sugar, amino acid isoflavonoid biosynthesis, flavone flavonol plant hormone signal translation, especially related isoflavone biosynthesis. addition, it also found bHLH MYB family transcription factors may be involved transduction bean. The above results will provide theoretical basis for help screening resources beans. Not applicable.

Язык: Английский

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