Public Perception of New Breeding Techniques (NBTs): A Multinational Statistical Analysis and Implications DOI

Tait R. Medina

Опубликована: Апрель 21, 2025

Abstract New Breeding Techniques (NBTs), such as CRISPR-Cas9, TALENs, and ODM, are reshaping the way we develop plant-based products for food cosmetic applications by allowing more precise efficient trait improvements. However, despite their scientific potential, global acceptance regulation of NBT-derived continue to vary widely. This study provides a comparative analysis public perceptions across six countries—Spain, France, China, Japan, Brazil, United States—focusing on consumer attitudes toward NBT use in skincare products. Based survey data from 724 participants, found clear regional differences familiarity, risk perception, NBTs. Participants China Japan showed relatively higher awareness openness these technologies. In contrast, respondents Spain France were skeptical, especially when it came environmental risks. opinions evenly split, reflecting ongoing national discussions. Despite U.S. having product-based regulatory framework, trust safety willingness consume goods was notably low. One most consistent findings strong support mandatory labeling all countries, signaling shared expectation transparency. Claims framing “green” or sustainable received limited agreement, particularly Western countries. Statistical analyses confirmed significant response patterns, although average scores did not significantly. These results point importance considering social cultural context development communication innovations. While is gradually increasing, broader adoption will depend transparent regulation, locally adapted strategies, genuine engagement. Building through clear, evidence-based messaging—and respecting values concerns—will be essential ensuring responsible widely accepted genome-edited

Язык: Английский

Viral-Based Gene Editing System for Nutritional Improvement of Fructan Content in Lettuce DOI Open Access
Yarin Livneh,

Dor Agmon,

Ehud Leor‐Librach

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2594 - 2594

Опубликована: Март 13, 2025

Lettuce is a globally cultivated and consumed leafy crop. Here we developed an efficient tobacco rattle virus (TRV)-based guide RNA (gRNA) delivery system for CRISPR/Cas editing in the commercial lettuce cultivar ‘Noga’. Plants stably expressing Cas9 were inoculated with TRV vectors carrying gRNAs targeting five nutrient-associated genes. The achieved average efficiency of 48.7%, up to 78.9% regenerated plantlets showing independent mutations. This approach eliminates need antibiotic selection, simplifying tissue culture processes. supports diverse applications, including Cas12a large-fragment deletions using dual gRNA sets. Targeting fructan 1-exohydrolase 2 (1-FEH2) gene produced knockout lines significant increases prebiotic dietary fibre content, 5.2-fold, rise degree polymerisation by 2.15 units compared controls. Combining 1-FEH1 1-FEH2 knockouts did not further increase levels, revealing as predominant isozyme lettuce. RT-qPCR analysis showed reduced expression upstream biosynthetic enzyme sucrose:sucrose 1-fructosyl transferase (1-SST), suggesting potential feedback inhibition metabolism. TRV-based approach, utilised here could be applied improve other valuable traits lettuce, may inspire similar systems enhance nutritional content crops.

Язык: Английский

Процитировано

0
Public Perception of New Breeding Techniques (NBTs): A Multinational Statistical Analysis and Implications DOI

Tait R. Medina

Опубликована: Апрель 21, 2025

Abstract New Breeding Techniques (NBTs), such as CRISPR-Cas9, TALENs, and ODM, are reshaping the way we develop plant-based products for food cosmetic applications by allowing more precise efficient trait improvements. However, despite their scientific potential, global acceptance regulation of NBT-derived continue to vary widely. This study provides a comparative analysis public perceptions across six countries—Spain, France, China, Japan, Brazil, United States—focusing on consumer attitudes toward NBT use in skincare products. Based survey data from 724 participants, found clear regional differences familiarity, risk perception, NBTs. Participants China Japan showed relatively higher awareness openness these technologies. In contrast, respondents Spain France were skeptical, especially when it came environmental risks. opinions evenly split, reflecting ongoing national discussions. Despite U.S. having product-based regulatory framework, trust safety willingness consume goods was notably low. One most consistent findings strong support mandatory labeling all countries, signaling shared expectation transparency. Claims framing “green” or sustainable received limited agreement, particularly Western countries. Statistical analyses confirmed significant response patterns, although average scores did not significantly. These results point importance considering social cultural context development communication innovations. While is gradually increasing, broader adoption will depend transparent regulation, locally adapted strategies, genuine engagement. Building through clear, evidence-based messaging—and respecting values concerns—will be essential ensuring responsible widely accepted genome-edited

Язык: Английский

Процитировано

0