Photochemical & Photobiological Sciences, Год журнала: 2024, Номер unknown
Опубликована: Сен. 17, 2024
Язык: Английский
Journal of Biotechnology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Applied and Environmental Microbiology, Год журнала: 2025, Номер unknown
Опубликована: Фев. 27, 2025
ABSTRACT Oyster mushrooms are grown commercially worldwide, especially in many developing countries, for their easy cultivation and high biological efficiency. Cap color is an important commercial trait oyster mushrooms. Little known about the genetic mechanism of cap mushrooms, which limits molecular breeding improvement color-type cultivars. In this study, candidate gene, pcmfs , mushroom Pleurotus cornucopiae was identified based on results QTL (quantitative loci) mapping comparative transcriptome analysis our previous research. The gene belonged to major facilitator superfamily (MFS) localized cell membrane. Expression pattern overexpression experiment demonstrated that played positive role formation, with expression levels leading dark color. To knowledge, first reported may be involved melanin transport edible fungi. will enhance understanding basis formation ultimately facilitating targeted phenotypic trait. IMPORTANCE widely cultivated particularly owing straightforward requirements represents a significant Despite its significance, remains poorly understood, limiting progress diversify variants. Bridging knowledge gap could improve market appeal consumer satisfaction these cultivars by strategies. research, P. mapped DEGs (differentially expressed genes) between strains white were identified. Based this, further mined. belonging (MFS), experiments have shown plays formation. contribute elucidate mechanisms governing advancing
Язык: Английский
Процитировано
0
Applied Microbiology and Biotechnology, Год журнала: 2025, Номер 109(1)
Опубликована: Март 11, 2025
In this study, the biological applications of cultivation methods related to cultivar selection, vegetative growth, and reproductive development in Lentinula edodes are briefly reviewed clarify current situation inform future developments. The cultivars widely used main production areas derived from wild strains distributed northern Asia. most effective techniques for identification molecular markers identified two nuclear genome datasets one mitochondrial dataset. stage breeding is at junction Breeding 3.0 (biological breeding) 4.0 (intelligent breeding). Plant breeder's rights patents have different emphases on new variety protection, with former being utilized globally. L. mostly produced synthetic logs filled sawdust substrates. Hardwood comprises approximately 80% growth involves distinct stages mycelial colonization browning. Mycelia mainly perform glycolysis, tricarboxylic acid cycle, respiratory metabolism reactions produce energy intermediates synthesizing structural components hyphae stage. Upon stimulation by physiological environmental pressures after colonization, mycelia trigger gluconeogenesis, autophagy, secondary metabolism, increase metabolic flux pentose phosphate pathway, activate glyoxylate accumulate melanin surface ensure survival. Sexually competent can form hyphal knots as a result reprogrammed branching patterns period (which varies cultivar) specific factors. Under genetically encoded developmental program, undergo aggregation, tissue differentiation, primordium formation, meiosis hymenium, stipe elongation, basidiospore maturation, cap expansion mature fruiting bodies. Growers achieve good body shape high yield regulating number young bodies adjusting • Cultivar selection becomes less increasing technological requirement cultivation. showed events browning stages. Specific breading may be next milestone
Язык: Английский
Процитировано
0
Journal of Food Measurement & Characterization, Год журнала: 2025, Номер unknown
Опубликована: Апрель 7, 2025
Язык: Английский
Процитировано
0
Farmaciya (Pharmacy), Год журнала: 2024, Номер unknown, С. 39 - 47
Опубликована: Сен. 1, 2024
Introduction. The article provides information on the functional properties of various melanins and their biosynthesis, as well natural sources production, use medicines dietary supplements containing melanin in medical practice. To do this, a systematic search was conducted databases: CyberLeninka, eLibrary, PubMed, SAGE Premier, Springer, Wiley Journals, over past 10 years (2013–2023). As result review, it revealed that are widespread living organisms an important biomaterial. Melanins microbial, fungal, animal plant origin known, they divided into five types, which most studied eumelanin, pheomelanin neuromelanin. A number studies presented prove characterized by wide range biological activity. They have photoprotective, radioprotective, antimutagenic, immunomodulatory stress-protective, geroprotective, antioxidant, detoxification anti-inflammatory effects. Information is provided possible complex therapy melanoma, Parkinson's disease neurodegenerative diseases, to protect skin eyes from radiation damage, correct stress conditions prevent infectious diseases during epidemic season. It has been shown chaga widely used domestic medicine form (Befungin) supplements. An difference between them concentration melanin, method its isolation purification, manufacturing technology, demonstrated article. biologically active additive with additional source β-glucans polyphenols Amelan considered, main component fungal obtained increases yield improves quality increasing paramagnetic centers compared without specified pretreatment fruit bodies. Conclusion. indicates prospects using for production additives.
Язык: Английский
Процитировано
2
Microbial Cell Factories, Год журнала: 2024, Номер 23(1)
Опубликована: Дек. 19, 2024
Fungi can synthesize a diverse range of melanins with appropriate physicochemical and biological characteristics for numerous applications in health, environmental protection, energy, industry. Gaining deeper insights into the chemical structures, biosynthetic pathways, regulatory mechanisms fungal melanin would establish basis metabolic engineering approaches, aimed at enhancing production efficiency creating custom-designed desirable material properties. Due to growing interest their beneficial effects applications, research on structure, biosynthesis, regulation has significantly advanced. This review highlighted recent progress concentrating networks, suggested how an improved understanding biosynthesis could enable efficient future applications.
Язык: Английский
Процитировано
2
Journal of Fungi, Год журнала: 2023, Номер 9(3), С. 311 - 311
Опубликована: Фев. 28, 2023
Dihydroxynapthalene-(DHN) and L-dihydroxyphenylalanine (L-DOPA) are two types of dominant melanin in fungi. Fungal melanins with versatile functions frequently associated pathogenicity stress tolerance. In rice blast fungus, Magnaporthe oryzae, DHN is essential to maintain the integrity infectious structure, appressoria; but role tyrosinase-derived L-DOPA still unknown. Here, we have genetically biologically characterized a tyrosinase gene (MoTyr) M. oryzae. MoTyr encodes protein 719 amino acids that contains typical CuA CuB domains tyrosinase. The deletion mutant (ΔMoTyr) was obtained by using homologous recombination approach. Phenotypic analysis showed conidiophore stalks conidia formation significantly reduced ΔMoTyr. Under different concentrations glycerol PEG, more appressoria collapsed strains than wild type, suggesting appressorium wall. Melanin measurement confirmed loss resulted significant decrease synthesis. Accordingly, stunted germination under conditions. Importantly, affected both infection pathogenesis stages. These results suggest MoTyr, like pigment synthase, plays key formation, integrity, revealing potential drug target for disease control.
Язык: Английский
Процитировано
6
International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(11), С. 9689 - 9689
Опубликована: Июнь 2, 2023
A unique feature of nanoparticles for bio-application is the ease achieving multi-functionality through covalent and non-covalent functionalization. In this way, multiple therapeutic actions, including chemical, photothermal photodynamic activity, can be combined with different bio-imaging modalities, such as magnetic resonance, photoacoustic, fluorescence imaging, in a theragnostic approach. context, melanin-related nanomaterials possess features since they are intrinsically biocompatible and, due to their optical electronic properties, themselves very efficient agents, antioxidants, photoacoustic contrast agents. Moreover, these materials present versatility functionalization, which makes them ideal design multifunctional platforms nanomedicine integrating new functions drug delivery controlled release, gene therapy, or ability resonance imaging. review, most relevant recent examples melanin-based multi-functionalized nanosystems discussed, highlighting methods functionalization particular, distinguishing pre-functionalization post-functionalization. meantime, properties melanin coatings employable variety material substrates also briefly introduced, especially order explain origin final part, critical issues related that may arise during melanin-like nanoplatforms listed discussed.
Язык: Английский
Процитировано
6
Frontiers in Bioengineering and Biotechnology, Год журнала: 2023, Номер 11
Опубликована: Сен. 13, 2023
Melanin is one of the most abundant natural biomolecules on Earth. These macromolecular biopolymers display several unique physical and chemical properties have garnered interest as biomaterials for various commercial industrial applications. To this end, extensive research has gone into refining methods synthesis extraction melanin from recombinant sources. In study, we developed refined a procedure using microbial system biosynthesis tyrosinase enzyme Tyr1 tyrosine substrate. Using emergent chassis organisms Vibrio natriegens , achieved maximal yields 7.57 g/L, highest reported volumetric productivities 473 mg L −1 h with 100% conversion rates in an optimized, minimally defined medium. Additionally, identified investigated use native copper responsive promoter V. stringent regulation heterologous protein expression cost effective alternative to traditional IPTG-based induction. This represents promising advancement towards green, rapid, economical biomanufacture melanin.
Язык: Английский
Процитировано
6
Frontiers in Microbiology, Год журнала: 2023, Номер 14
Опубликована: Сен. 29, 2023
Morchella sextelata , a highly sought-after edible mushroom worldwide, is evaluated based on its cap color as an essential commercial property indicator. In the present study, effects of blue light pigmentation in M. well synthesis and structural characteristics melanin pigments within were examined. The results showed that increase proportion lighting environment promoted melanization cap. Transmission scanning electron microscopy revealed localization mycelium ultrastructural characteristics. UV–visible analysis demonstrated exhibited maximum absorption peak at 220 nm possessed high alkaline solubility acid precipitability. analyzed using FTIR, NMR, HPLC, elemental analysis, which confirmed presence eumelanin, pheomelanin, allomelanin both brown black caps. Furthermore, can stimulate eumelanin pheomelanin. obtained serve foundation for comprehending mechanism by regulates formation mushrooms.
Язык: Английский
Процитировано
5