Preparative Biochemistry & Biotechnology,
Год журнала:
2023,
Номер
54(6), С. 796 - 808
Опубликована: Дек. 23, 2023
sp.
PM06,
previously
isolated
from
sugarcane
waste
pressmud,
could
produce
dual
enzymes
α-amylase
and
cellulase.
The
isolate's
crude
were
purified
homogeneously
using
ammonium
sulfate
precipitation
followed
by
High
Quaternary
amine
anion
exchange
chromatography.
Purified
revealed
the
molecular
weights
of
cellulase
as
55
52
kDa,
with
a
purification
fold
15.4
11.5,
respectively.
specific
activity
740.7
555.6
U/mg,
It
demonstrated
wide
range
pH
5.0
to
8.5,
an
optimum
5.5
6.4
for
temperature
was
50
°C
60
kinetic
parameters
741.5
±
3.75
µmol/min/mg
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Янв. 31, 2025
Egg
protein
(EP)
concentration,
pH,
and
glutaraldehyde
(GA)
concentration
were
optimized
using
Box
Behnken
design
(BBD)
to
prepare
GA-EP-Carr
(Carrageenan)
beads
as
a
carrier
for
Aspergillus
niger
MK981235
cellulase.
It
was
recommended
that
the
concentrations
of
GA,
EP
be
set
at
11.21%
(w/v),
8%
(w/w),
pH
3,
respectively.
determined
60
°C
2%
free
form
3%
im-cellulase
optimum
temperature
CMC
parameters
maximum
enzyme
activity.
Free
have
Km
Vmax
2.22
mg.ml-1
1.76
µmol.ml-1.min-1,
4.55
3.33
Covalent
coupling
A.
cellulase
GA-
EP-
Carr
improved
its
thermodynamic
T1/2
D-values
by
2.48,
2.01,
2.36
times
40,
50,
°C,
100%
active
days
4
can
used
hydrolysis
20
successive
cycles.
showed
remarkable
efficiency
in
clarification
mango,
peach,
grape,
orange
juices
emphasized
TSS
(total
soluble
solids),
turbidity,
reducing
sugar
measurements
3
applied
with
high
juice
industry.
Preparative Biochemistry & Biotechnology,
Год журнала:
2025,
Номер
unknown, С. 1 - 18
Опубликована: Янв. 21, 2025
Halophilic
bacteria
are
promising
candidates
for
biofuel
production
because
of
their
efficient
cellulose
degradation.
Their
cellulases
exhibit
high
activity,
even
in
the
presence
inhibitors
and
under
extreme
conditions,
making
them
ideal
biorefinery
applications.
In
this
study,
we
isolated
a
strain
Halomonas
elongata
(Kadal6)
from
decomposed
cotton
cloth
on
Rameshwaram
seashore.
Morphological,
biochemical,
16S
rRNA
analyses
revealed
that
Kadal6
was
99.93%
similar
to
cellulase-producing
strain,
H.
MH25661.
The
tolerance
cellulase
assessed
through
molecular
docking
with
model
MH25661
generated
by
I-TASSER
experimentally
using
response
surface
methodology
(RSM)
Kadal6.
A
study
indicated
inhibition
constant
ethanol,
hydroxymethylfurfural
(HMF),
furfural.
Cellulase
(CellHe)
showed
maximum
rate
44.27%
at
55
°C,
15%
6.5
g/L
furfural
HMF.
enzyme
retained
50%
its
activity
these
inhibitors,
remained
unaffected
1
HMF,
although
occurred
3
g/L.
demonstrated
significant
both
vitro
silico,
indicating
potential
applications
harsh
environments.
Food Bioscience,
Год журнала:
2024,
Номер
59, С. 103944 - 103944
Опубликована: Март 22, 2024
Plant-derived
esterase
is
a
promising
alternative
enzyme
source
for
pesticide
residue
analysis.
The
present
study
aimed
to
obtain
purified
red
kidney
bean
(RKBE),
investigate
its
biochemical
properties
and
application
potential
in
assay.
RKBE
was
extracted
by
simple
aqueous
two-phase
extraction
combined
with
dextran
gel
filtration
chromatography,
specific
activity
of
11.845
U/mg
purification
fold
18.925.
SDS-PAGE
revealed
that
has
molecular
weight
33
kDa.
Preliminary
structural
characterization
showed
the
amino
acid
sequence
similar
α/β-hydrolase
(ESW18998)
derived
from
Phaseolus
vulgaris
19.5%
coverage,
relative
contents
β-sheet,
β-turning,
α-helix,
irregularly
coil
were
22.69%,
24.19%,
30.12%,
23.00%,
respectively.
optimal
pH
temperature
6.5
40
°C.
Ca2+,
Mg2+,
Zn2+,
low
concentrations
methanol
ethanol
stimulated
activity.
half-life
138.6
days
at
−20
Kinetic
studies
exhibited
high
affinity
catalytic
towards
1-naphthyl
acetate,
Km
0.277
mM
Kcat/Km
0.455
s−1
mM−1.
Enzyme-specific
inhibition
assay
indicated
be
carboxylesterase.
Finally,
susceptibility
experiments
confirmed
organophosphorus
carbamate
pesticides
exerted
inhibitory
effects
on
For
methamidophos,
methomyl,
dichlorvos,
carbaryl,
limit
detection
(IC10)
values
cabbage
0.0031,
0.0045,
0.001,
0.0065
mg/kg,
respectively,
suggesting
can
serve
as
new,
widely
available
cost-effective
residual
foods.
AIMS Microbiology,
Год журнала:
2024,
Номер
10(1), С. 187 - 219
Опубликована: Янв. 1, 2024
Actinomycetes
are
an
attractive
source
of
lignocellulose-degrading
enzymes.
The
search
for
actinomycetes
producing
extremozyme
cellulase
using
cheap
lignocellulosic
waste
remains
a
priority
goal
enzyme
research.
In
this
context,
the
extremophilic
actinomycete
NBRM9
showed
promising
cellulolytic
activity
in
solid
and
liquid
assays.
This
was
identified
as
