Preparative Biochemistry & Biotechnology,
Год журнала:
2023,
Номер
54(6), С. 796 - 808
Опубликована: Дек. 23, 2023
sp.
PM06,
previously
isolated
from
sugarcane
waste
pressmud,
could
produce
dual
enzymes
α-amylase
and
cellulase.
The
isolate's
crude
were
purified
homogeneously
using
ammonium
sulfate
precipitation
followed
by
High
Quaternary
amine
anion
exchange
chromatography.
Purified
revealed
the
molecular
weights
of
cellulase
as
55
52
kDa,
with
a
purification
fold
15.4
11.5,
respectively.
specific
activity
740.7
555.6
U/mg,
It
demonstrated
wide
range
pH
5.0
to
8.5,
an
optimum
5.5
6.4
for
temperature
was
50
°C
60
kinetic
parameters
741.5
±
3.75
µmol/min/mg
Applied Sciences,
Год журнала:
2024,
Номер
14(20), С. 9295 - 9295
Опубликована: Окт. 12, 2024
The
interest
in
microbial
pectinases
is
increasing
due
to
their
use
the
biotechnological
and
food
industries
response
global
demand.
In
this
study,
among
22
halophilic
bacterial
strains
screened,
20
showed
pectinase
activity.
highest
activity
was
measured
for
Virgibacillus
salarius
strain
434.
As
production
meets
many
parameter
optimization
constraints,
optimal
conditions
maximizing
yield
of
synthesized
using
investigated
were
followed.
Enzyme
purification
accomplished
at
up
11.5-fold
via
ultrafiltration
gel
filtration
chromatography,
until
enzyme’s
specific
had
increased
10-fold
(104.3
U/mg).
molecular
weight
determined
be
68
kDa.
Km
Vmax
values
0.38
mg/mL
120
U/mg,
respectively,
determined.
purified
from
434
good
thermal
stability.
A
half-life
min
70
°C
high
catalytic
under
haloalkaline
(pH
9
g
L−1
NaCl)
observed.
Its
storage
stability
−20
exceeded
2
months.
This
demonstrates
its
potential
application
where
these
extremes
are
present,
as
harsh
industrial
processes
require
novel
with
unique
properties
instead
hazardous
chemicals.
Preparative Biochemistry & Biotechnology,
Год журнала:
2023,
Номер
54(6), С. 796 - 808
Опубликована: Дек. 23, 2023
sp.
PM06,
previously
isolated
from
sugarcane
waste
pressmud,
could
produce
dual
enzymes
α-amylase
and
cellulase.
The
isolate's
crude
were
purified
homogeneously
using
ammonium
sulfate
precipitation
followed
by
High
Quaternary
amine
anion
exchange
chromatography.
Purified
revealed
the
molecular
weights
of
cellulase
as
55
52
kDa,
with
a
purification
fold
15.4
11.5,
respectively.
specific
activity
740.7
555.6
U/mg,
It
demonstrated
wide
range
pH
5.0
to
8.5,
an
optimum
5.5
6.4
for
temperature
was
50
°C
60
kinetic
parameters
741.5
±
3.75
µmol/min/mg
