flgL mutation reduces pathogenicity of Aeromonas hydrophila by negatively regulating swimming ability, biofilm forming ability, adherence and virulence gene expression

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 261, С. 129676 - 129676

Опубликована: Янв. 23, 2024

Язык: Английский

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The role and function mechanism of pilV in modulating the pathogenicity of Aeromonas hydrophila

Aquaculture, Год журнала: 2025, Номер unknown, С. 742258 - 742258

Опубликована: Фев. 1, 2025

Язык: Английский

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Effects of oral immunization with Bacillus subtilis displaying Vibrio harveyi FlgE protein on the intestinal structure and gut microbiota of grouper

Fish & Shellfish Immunology, Год журнала: 2025, Номер 160, С. 110234 - 110234

Опубликована: Фев. 24, 2025

Язык: Английский

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Inhibitors of Lysinoalanine Cross-Linking in the Flagella Hook as Antimicrobials against Spirochetes

ACS Chemical Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 25, 2025

Spirochetes are especially invasive bacteria that responsible for several human diseases, including Lyme disease, periodontal syphilis, and leptospirosis. rely on an unusual form of motility based periplasmic flagella (PFs) to infect hosts evade the immune system. The flexible hook these PFs contains a post-translational modification in lysinoalanine (Lal) cross-link between adjacent subunits FlgE, which primarily comprise hook. Lal cross-linking has since been found key species across phylum involves residues highly conserved. requirement pathogens Treponema denticola (Td) Borreliella burgdorferi (Bb) establish as potential therapeutic target development antimicrobials. Herein, we present design, development, application NanoLuc-based high-throughput screen was used successfully identify two structurally related inhibitors (hexachlorophene triclosan) from library clinically approved small molecules. A structure–activity relationship study further expanded inhibitor set third compound (dichlorophene), each demonstrated biochemically block autocatalytic FlgE pathogenic spirochetes with varied mechanisms degrees specificity. most potent inhibitor, hexachlorophene, alters cultured cells Td reduces bacterial swimming plate assays. Overall, results provide proof-of-concept discovery Lal-cross-link combat spirochete-derived illnesses.

Язык: Английский

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Transcriptomic Analysis of the Negative Effect of Epigallocatechin-3-Gallate from Tea Plant (Camellia sinensis) on Agrobacterium-Mediated Transformation Efficiency

Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(3), С. 178 - 178

Опубликована: Март 8, 2025

Agrobacterium-mediated transformation is a widely used method for plant genetic modification. However, its efficiency in tea plants notably low, and the underlying molecular mechanisms remain unclear, hindering advancements breeding biology of plants. In this study, tobacco was utilized as model to investigate effects various concentrations epigallocatechin-3-gallate (EGCG) on Agrobacterium efficiency. The results demonstrated that at an EGCG concentration 0.4 mg/mL, nearly lost ability transform tobacco. Additionally, malondialdehyde content measured before after treatment. findings indicated treatment led increase content. Transcriptome sequencing analysis revealed differentially expressed genes (DEGs) involved flagellar synthesis secretion systems were down-regulated under stress. Furthermore, flgE, virB4, virB6 identified hub through weighted gene co-expression network (WGCNA). These elucidate dynamic by which affects both physicochemical levels, providing theoretical basis optimizing

Язык: Английский

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Contribution of the type IV pili secretin tapQ to motility, growth, adhesion, stress tolerance and virulence of Aeromonas hydrophila

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142203 - 142203

Опубликована: Март 1, 2025

Язык: Английский

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Strong Opponent of Walnut Anthracnose—Bacillus velezensis and Its Transcriptome Analysis

Microorganisms, Год журнала: 2023, Номер 11(8), С. 1885 - 1885

Опубликована: Июль 26, 2023

Walnut is a significant economic tree species worldwide. anthracnose, caused by the pathogen Colletotrichum gloeosporioides, greatly reduces walnut production and benefits. Our study showed that Bacillus velezensis effectively halted growth of C. inducing noticeable abnormalities such as hyphal breakage distortion, thereby curtailing pathogen’s virulence. A 50–100 times dilution B. fermentation broth, applied every two to three days, served an efficient protective layer for leaves fruits against gloeosporioides infection. Transcriptomic analysis unveiled its dynamic response gloeosporioides. On second day, upregulated number differentially expressed genes related synthesis metabolic products, amino acid biosynthesis, motility. fourth continuous products acids, along with differential expression spore-related genes, was observed. By sixth focus shifted towards environmental adaptation carbon source utilization. Throughout process, likely employed strategies release increased chemotaxis, nutrient competition exert antagonistic effect on Fluorescence quantitative results 15 primer pairs were up-regulated down-regulated, 100% similarity rate transcriptome sequencing results, confirming their authenticity. These findings provided foundation widespread application biocontrol agent in agriculture forestry.

Язык: Английский

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Antimicrobial activity of magnolol against Bacillus cereus and its application as food preservative

Innovative Food Science & Emerging Technologies, Год журнала: 2024, Номер 95, С. 103746 - 103746

Опубликована: Июль 1, 2024

Язык: Английский

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Bisphenol S Promotes the Transfer of Antibiotic Resistance Genes via Transformation

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(18), С. 9819 - 9819

Опубликована: Сен. 11, 2024

The antibiotic resistance crisis has seriously jeopardized public health and human safety. As one of the ways horizontal transfer, transformation enables bacteria to acquire exogenous genes naturally. Bisphenol compounds are now widely used in plastics, food, beverage packaging, have become a new environmental pollutant. However, their potential relationship with spread (ARGs) environment remains largely unexplored. In this study, we aimed assess whether ubiquitous bisphenol S (BPS) could promote plasmid-borne ARGs. Using plasmid pUC19 carrying ampicillin gene as an extracellular ARG model microorganism

Язык: Английский

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Phylogenetic analysis of pathogenic genes in Helicobacter species

Shijie huaren xiaohua zazhi, Год журнала: 2024, Номер 32(1), С. 58 - 70

Опубликована: Янв. 26, 2024

Язык: Английский

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