Applied and Environmental Microbiology, Год журнала: 2024, Номер 90(4)
Опубликована: Март 17, 2024
ABSTRACT Per- and polyfluoroalkyl substances (PFAS) are highly fluorinated synthetic organic compounds that have been used extensively in various industries owing to their unique properties. The PFAS family encompasses diverse classes, with only a fraction being commercially relevant. These found the environment, including water sources, soil, wildlife, leading human exposure fueling concerns about potential health impacts. Although degradation is challenging, biodegradation offers promising, eco-friendly solution. Biodegradation has effective for variety of contaminants but yet be successful due paucity identified microbial species capable transforming these compounds. Recent studies investigated biotransformation fluoride release; however, number specific microorganisms enzymes demonstrable activity remains limited. This review discusses could metabolism, haloacid dehalogenases, reductive cytochromes P450, alkane butane monooxygenases, peroxidases, laccases, desulfonases, mechanisms resistance intracellular fluoride. Finally, we emphasize enzyme engineering advance strategies provide insights future research this field.
Язык: Английский
Процитировано
11Bacterial degradation of perfluoroalkyl acids
Current Opinion in Biotechnology, Год журнала: 2024, Номер 88, С. 103170 - 103170
Опубликована: Июль 16, 2024
Advances in biological degradation of per- and polyfluoroalkyl substances (PFAS) have shown that bioremediation is a promising method PFAS mineralization; however, most these studies focus on remediation more reactive polyfluorinated compounds. This review focuses the defluorination recalcitrant perfluorinated alkyl acids (PFAAs) by bacteria. We highlight key report PFAA products, specific bacteria, relevant genes. Among studies, we discuss trends anaerobic versus aerobic conditions with bacterial species or consortia. holistic seeks to elucidate state biodegradation research need for future environmental application.
Язык: Английский
Процитировано
10Beyond Conventional Antifungals: Combating Resistance Through Novel Therapeutic Pathways
Pharmaceuticals, Год журнала: 2025, Номер 18(3), С. 364 - 364
Опубликована: Март 4, 2025
The rising burden of fungal infections presents a significant challenge to global healthcare, particularly with increasing antifungal resistance limiting treatment efficacy. Early detection and timely intervention remain critical, yet pathogens employ diverse mechanisms evade host immunity develop resistance, undermining existing therapeutic options. Limited options necessitate novel strategies. This review provides comprehensive overview conventional agents, their action, emerging pathways. Furthermore, it highlights recently approved investigational compounds while evaluating innovative approaches such as nanotechnology, drug repurposing, immunotherapy. Addressing requires multifaceted strategy that integrates therapeutics, enhanced diagnostic tools, future research efforts sustainable effective solutions.
Язык: Английский
Процитировано
1Filamentous Fungi as Bioremediation Agents of Industrial Effluents: A Systematic Review
Fermentation, Год журнала: 2024, Номер 10(3), С. 143 - 143
Опубликована: Март 1, 2024
The industrial sector plays a significant role in global economic growth. However, it also produces polluting effluents that must be treated to prevent environmental damage and ensure the quality of life for future generations is not compromised. Various physical, chemical, biological methods have been employed treat effluents. Filamentous fungi, particular, garnered attention as effective bioremediation agents due their ability produce enzymes capable degrading recalcitrant compounds, adsorb different pollutant molecules. novelty work reported herein lies its comprehensive assessment research surrounding use white- brown-rot fungi removing phenolic compounds from This study employs systematic review coupled with scientometric analysis provide insights into evolution this technology over time. It scrutinizes geographical distribution, identifies gaps trends, highlights most studied fungal species applications. A 464 publications 1945 2023 assessed these White-rot were predominant (96.3%), notably Phanerochaete chrysosporium, Pleurotus ostreatus, Trametes versicolor, Lentinula edodes. cultures employing free cells (64.15%) stand out those using immobilized cells, just like isolated regarding systems microbial consortia. Geographically, Italy, Spain, Greece, India, Brazil emerged prominent countries related area during evaluated period.
Язык: Английский
Процитировано
7Hazards Associated with the Combined Application of Fungicides and Poultry Litter in Agricultural Areas
Journal of Xenobiotics, Год журнала: 2024, Номер 14(1), С. 110 - 134
Опубликована: Янв. 9, 2024
In recent decades, the poultry farming industry has assumed a pivotal role in meeting global demand for affordable animal proteins. While makes substantial contribution to food security and nutrition, it also presents environmental public health challenges. The use of litter as fertilizer agricultural soils raises concerns about transfer pathogens drug-resistant microorganisms from farms crop production areas. On other hand, according Food Agriculture Organization United Nations (FAO), fungicides represent second most used chemical group practices. this context, receive application both production. This practice can result fungal contamination soil development antifungal resistance. article explores necessity monitoring resistance, particularly areas with co-application fungicides. It highlights fungi ecosystems, decomposition, mutualistic plant associations. We call interdisciplinary research comprehensively understand resistance environment. approach seeks promote sustainability realms human health, agriculture, environment, aligning seamlessly One Health concept.
Язык: Английский
Процитировано
6Whole Genome Identification and Biochemical Characteristics of the Tilletia horrida Cytochrome P450 Gene Family
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(19), С. 10478 - 10478
Опубликована: Сен. 28, 2024
Rice kernel smut caused by the biotrophic basidiomycete fungus Tilletia horrida causes significant yield losses in hybrid rice-growing areas around world. Cytochrome P450 (CYP) enzyme is a membrane-bound heme-containing monooxygenase. In fungi, CYPs play role cellular metabolism, adaptation, pathogenicity, decomposition, and biotransformation of hazardous chemicals. this study, we identified 20 CYP genes based on complete sequence analysis functional annotation from T. JY-521 genome. The subcellular localization, conserved motifs, structures these were further predicted. ThCYP exhibit differences gene protein motifs. Subcellular localization showed that they located plasma membrane, cytoplasm, nucleus, mitochondria, extracellular space, indicating had multiple functions. Some cis-regulatory elements related to stress response plant hormones found promoter regions genes. Protein–protein interaction (PPI) several proteins interact with involved ergosterol pathway. Moreover, expression different responses infection time points underwent dynamic changes during infection, rice their potential pathogenic mechanism. These results provided valuable resources for elucidating structure family laid an important foundation research roles pathogenesis.
Язык: Английский
Процитировано
6Innovative Approaches in Extremophile-Mediated Remediation of Toxic Pollutants: A Comprehensive Review
Water Conservation Science and Engineering, Год журнала: 2024, Номер 9(2)
Опубликована: Июль 2, 2024
Язык: Английский
Процитировано
5Pharmaceutical removal from wastewater by introducing cytochrome P450s into microalgae
Microbial Biotechnology, Год журнала: 2024, Номер 17(6)
Опубликована: Июнь 1, 2024
Pharmaceuticals are of increasing environmental concern as they emerge and accumulate in surface- groundwater systems around the world, endangering overall health aquatic ecosystems. Municipal wastewater discharge is a significant vector for pharmaceuticals their metabolites to enter surface waters humans incompletely absorb prescription drugs excrete up 50% into wastewater, which subsequently removed during treatment. Microalgae present promising target improving treatment due ability remove some pollutants efficiently. However, inherent metabolic pathways limit capacity degrade more recalcitrant organic compounds such pharmaceuticals. The human liver employs enzymes break down drugs, these extensively researched drug development, meaning cytochrome P450 responsible metabolizing each approved well studied. Thus, unlocking or expression endogenous microalgae could be cost-effective strategy reduce pharmaceutical loads effluents. Here, we discuss challenges opportunities associated with introducing microalgae. We anticipate that P450-engineered can serve new removal method sustainable solution upgrade facilities function "mega livers".
Язык: Английский
Процитировано
4Fungal Biodegradation of Procyanidin in Submerged Fermentation
Fermentation, Год журнала: 2025, Номер 11(2), С. 75 - 75
Опубликована: Фев. 3, 2025
The coffee industry generates a large amount of waste that is difficult to treat due its chemical composition, namely, the presence caffeine and derivatives, as well recalcitrant molecules such tannins (mainly condensed or polymeric procyanidins), which make it an undervalued product. Procyanidins are compounds beneficial human health can be found in nature fruit, grain, seeds, beverages, among other foods. zero-waste approach has allowed for valorization by-products from food industry. Currently, pulp target research on extraction, purification, alternative use. Research fungal degradation procyanidins emerged avenue efficient use these by-products. In this study, biotransformation procyanidin evaluated comprises three steps: first, extraction partial purification pulp; second, production potential procyanidin-degrading enzyme by submerged fermentation with Aspergillus niger GH1; third, enzymatic extracellular extract evaluation using model system commercial C1. biodegradation/biotransformation results reveal formation new compounds, including final compound m/z 289, possibly monomeric molecule catechin epicatechin. Identification HPLC-MS confirmed C1 depletion under described assay conditions, could used understand biodegradation pathways proposed future study. Furthermore, confirm A. GH1 able degrade biotransform
Язык: Английский
Процитировано
0Biotransformation of oxybenzone and 3-(4-methylbenzylidene)camphor in Cunninghamella species: potential for environmental clean-up of widely used sunscreen agents
Journal of Hazardous Materials, Год журнала: 2025, Номер 489, С. 137544 - 137544
Опубликована: Фев. 11, 2025
Язык: Английский
Процитировано
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