Bacterial degradation of perfluoroalkyl acids

Current Opinion in Biotechnology, Год журнала: 2024, Номер 88, С. 103170 - 103170

Опубликована: Июль 16, 2024

Advances in biological degradation of per- and polyfluoroalkyl substances (PFAS) have shown that bioremediation is a promising method PFAS mineralization; however, most these studies focus on remediation more reactive polyfluorinated compounds. This review focuses the defluorination recalcitrant perfluorinated alkyl acids (PFAAs) by bacteria. We highlight key report PFAA products, specific bacteria, relevant genes. Among studies, we discuss trends anaerobic versus aerobic conditions with bacterial species or consortia. holistic seeks to elucidate state biodegradation research need for future environmental application.

Язык: Английский

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Toward the development of a molecular toolkit for the microbial remediation of per-and polyfluoroalkyl substances

Applied and Environmental Microbiology, Год журнала: 2024, Номер 90(4)

Опубликована: Март 17, 2024

ABSTRACT Per- and polyfluoroalkyl substances (PFAS) are highly fluorinated synthetic organic compounds that have been used extensively in various industries owing to their unique properties. The PFAS family encompasses diverse classes, with only a fraction being commercially relevant. These found the environment, including water sources, soil, wildlife, leading human exposure fueling concerns about potential health impacts. Although degradation is challenging, biodegradation offers promising, eco-friendly solution. Biodegradation has effective for variety of contaminants but yet be successful due paucity identified microbial species capable transforming these compounds. Recent studies investigated biotransformation fluoride release; however, number specific microorganisms enzymes demonstrable activity remains limited. This review discusses could metabolism, haloacid dehalogenases, reductive cytochromes P450, alkane butane monooxygenases, peroxidases, laccases, desulfonases, mechanisms resistance intracellular fluoride. Finally, we emphasize enzyme engineering advance strategies provide insights future research this field.

Язык: Английский

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Fungi for Sustainable Pharmaceutical Remediation: Enzymatic Innovations, Challenges, and Applications—A Review

Processes, Год журнала: 2025, Номер 13(4), С. 1034 - 1034

Опубликована: Март 31, 2025

The extensive use of pharmaceuticals in human and veterinary medicine has led to their persistent environmental release, posing ecological public health risks. Major sources include manufacturing effluents, excretion, aquaculture, improper disposal, contributing bioaccumulation ecotoxicity. Mycoremediation is the fungal-mediated biodegradation pharmaceuticals, offers a promising sustainable approach mitigate pharmaceutical pollution. Studies have reported that certain fungal species, including Trametes versicolor Pleurotus ostreatus, can degrade up 90% contaminants, such as diclofenac, carbamazepine, ibuprofen, within days weeks, depending on conditions. Fungi produce range extracellular enzymes, laccases peroxidases, alongside intracellular enzymes like cytochrome P450 monooxygenases, which catalyze transformation complex compounds. These play an essential role modifying, detoxifying, mineralizing xenobiotics, thereby reducing persistence toxicity. effectiveness biotransformation influenced by factors substrate specificity, enzyme stability, Optimal degradation typically occurs at pH 4.5–6.0 temperatures 20–30 °C. Recent advancements engineering, immobilization techniques, bioreactor design improved catalytic efficiency process feasibility. However, scaling fungal-based remediation systems for large-scale applications remains challenge. Addressing these limitations with synthetic biology, metabolic other biotechnological innovations could further enhance enzymatic pharmaceuticals. This review highlights innovations, applications, challenges mycoremediation, emphasizing potential fungi transformative solution waste management.

Язык: Английский

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Filamentous Fungi as Bioremediation Agents of Industrial Effluents: A Systematic Review

Fermentation, Год журнала: 2024, Номер 10(3), С. 143 - 143

Опубликована: Март 1, 2024

The industrial sector plays a significant role in global economic growth. However, it also produces polluting effluents that must be treated to prevent environmental damage and ensure the quality of life for future generations is not compromised. Various physical, chemical, biological methods have been employed treat effluents. Filamentous fungi, particular, garnered attention as effective bioremediation agents due their ability produce enzymes capable degrading recalcitrant compounds, adsorb different pollutant molecules. novelty work reported herein lies its comprehensive assessment research surrounding use white- brown-rot fungi removing phenolic compounds from This study employs systematic review coupled with scientometric analysis provide insights into evolution this technology over time. It scrutinizes geographical distribution, identifies gaps trends, highlights most studied fungal species applications. A 464 publications 1945 2023 assessed these White-rot were predominant (96.3%), notably Phanerochaete chrysosporium, Pleurotus ostreatus, Trametes versicolor, Lentinula edodes. cultures employing free cells (64.15%) stand out those using immobilized cells, just like isolated regarding systems microbial consortia. Geographically, Italy, Spain, Greece, India, Brazil emerged prominent countries related area during evaluated period.

Язык: Английский

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Innovative Approaches in Extremophile-Mediated Remediation of Toxic Pollutants: A Comprehensive Review

Water Conservation Science and Engineering, Год журнала: 2024, Номер 9(2)

Опубликована: Июль 2, 2024

Язык: Английский

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Beyond Conventional Antifungals: Combating Resistance Through Novel Therapeutic Pathways

Pharmaceuticals, Год журнала: 2025, Номер 18(3), С. 364 - 364

Опубликована: Март 4, 2025

The rising burden of fungal infections presents a significant challenge to global healthcare, particularly with increasing antifungal resistance limiting treatment efficacy. Early detection and timely intervention remain critical, yet pathogens employ diverse mechanisms evade host immunity develop resistance, undermining existing therapeutic options. Limited options necessitate novel strategies. This review provides comprehensive overview conventional agents, their action, emerging pathways. Furthermore, it highlights recently approved investigational compounds while evaluating innovative approaches such as nanotechnology, drug repurposing, immunotherapy. Addressing requires multifaceted strategy that integrates therapeutics, enhanced diagnostic tools, future research efforts sustainable effective solutions.

Язык: Английский

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Influence of Tree Species Composition on Leaf and Soil Properties and Soil Enzyme Activity in Mixed and Pure Oak (Quercus variabilis) Stands

Forests, Год журнала: 2025, Номер 16(3), С. 471 - 471

Опубликована: Март 7, 2025

There is an increasing number of studies providing evidence that multi-species tree plantations possess more advantages in terms species-specific diameter, growth rates, and soil properties than monocultures. In order to clarify the effect a plantation on leaf nutrition fertility, statistical analysis was carried out properties, as well enzyme activity, from two repeatedly measured stands Qingliangsi Forest District Dengfeng Station. For analysis, plots were categorized into type A B according different forest structures. Type mixed Quercus variabilis Platycladus orientalis, while pure stand variabilis. The results clearly showed water content P. orientalis greater Q. total water, free fatty acids, soluble sugar, flavonoid, tannin, lignin, cellulose, hemicellulose contents higher those A. Furthermore, moisture apparently stand. Soil peroxidase activity highest both among 10 activities. Meanwhile, there significant difference between catalase acid phosphatase urease, leucine aminopeptidase, sucrase significantly correlated with multiple addition, based correlation results, we noted had complex relationships activity. Compared monoculture stands, appeared have complicated preferable conservation capabilities. These further verify beneficial role holding capacity improving quality. broad-leaved deciduous species, evergreen coniferous species. Our study indicates these native Chinese species are suitable target when constructing forests. They can increase interaction enhance activities, improve soil.

Язык: Английский

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Hazards Associated with the Combined Application of Fungicides and Poultry Litter in Agricultural Areas

Journal of Xenobiotics, Год журнала: 2024, Номер 14(1), С. 110 - 134

Опубликована: Янв. 9, 2024

In recent decades, the poultry farming industry has assumed a pivotal role in meeting global demand for affordable animal proteins. While makes substantial contribution to food security and nutrition, it also presents environmental public health challenges. The use of litter as fertilizer agricultural soils raises concerns about transfer pathogens drug-resistant microorganisms from farms crop production areas. On other hand, according Food Agriculture Organization United Nations (FAO), fungicides represent second most used chemical group practices. this context, receive application both production. This practice can result fungal contamination soil development antifungal resistance. article explores necessity monitoring resistance, particularly areas with co-application fungicides. It highlights fungi ecosystems, decomposition, mutualistic plant associations. We call interdisciplinary research comprehensively understand resistance environment. approach seeks promote sustainability realms human health, agriculture, environment, aligning seamlessly One Health concept.

Язык: Английский

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Whole Genome Identification and Biochemical Characteristics of the Tilletia horrida Cytochrome P450 Gene Family

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(19), С. 10478 - 10478

Опубликована: Сен. 28, 2024

Rice kernel smut caused by the biotrophic basidiomycete fungus Tilletia horrida causes significant yield losses in hybrid rice-growing areas around world. Cytochrome P450 (CYP) enzyme is a membrane-bound heme-containing monooxygenase. In fungi, CYPs play role cellular metabolism, adaptation, pathogenicity, decomposition, and biotransformation of hazardous chemicals. this study, we identified 20 CYP genes based on complete sequence analysis functional annotation from T. JY-521 genome. The subcellular localization, conserved motifs, structures these were further predicted. ThCYP exhibit differences gene protein motifs. Subcellular localization showed that they located plasma membrane, cytoplasm, nucleus, mitochondria, extracellular space, indicating had multiple functions. Some cis-regulatory elements related to stress response plant hormones found promoter regions genes. Protein–protein interaction (PPI) several proteins interact with involved ergosterol pathway. Moreover, expression different responses infection time points underwent dynamic changes during infection, rice their potential pathogenic mechanism. These results provided valuable resources for elucidating structure family laid an important foundation research roles pathogenesis.

Язык: Английский

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Metabolic mechanism of lignin-derived aromatics in white-rot fungi

Applied Microbiology and Biotechnology, Год журнала: 2024, Номер 108(1)

Опубликована: Дек. 1, 2024

White-rot fungi, such as Phanerochaete chrysosporium, play a crucial role in biodegrading lignocellulosic biomass including cellulose, hemicellulose, and lignin. These fungi utilise various extracellular intracellular enzymes, lignin peroxidases, manganese versatile monooxygenases, dioxygenases, to degrade lignin-derived aromatics, thereby significantly contributing the global carbon cycle with potential applications industrial bioprocessing bioremediation. Although metabolism of fragments P. chrysosporium has been studied extensively, enzymes involved fragment conversion remain largely unknown. This review provides an overview current knowledge regarding metabolic pathways its by white-rot fungi. Recent studies have elucidated intricate regulatory mechanisms aromatic degradation focusing on flavoprotein intradiol homogentisate dioxygenase-like proteins, cytochrome P450 monooxygenases. Metabolic regulation these demonstrates adaptability degrading aromatics. The interplay between central pathways, haem biosynthesis, haem-dependent NAD(P)H regeneration highlights complexity insights improve our understanding fungal pave way for future aimed at leveraging sustainable biotechnological applications. KEY POINTS: • use lignin, cycle. Oxygenases are key converting adapt changes controlling TCA/glyoxylate bicycle.

Язык: Английский

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