Clinica Chimica Acta, Год журнала: 2021, Номер 523, С. 231 - 238
Опубликована: Окт. 8, 2021
Язык: Английский
Frontiers in Plant Science, Год журнала: 2020, Номер 11
Опубликована: Май 8, 2020
Cannabis (Cannabis sativa L.) is a complex, polymorphic plant species, which produces vast array of bioactive metabolites, the two major chemical groups being cannabinoids and terpenoids. Nonetheless, psychoactive cannabinoid tetrahydrocannabinol (Δ9-THC) non-psychoactive cannabidiol (CBD), are that have monopolized research interest. Currently, more than 600 varieties commercially available, providing access to multitude potent extracts with complex compositions, whose genetics largely inconclusive. Recently introduced legislation on cultivation in many countries represents great opportunity, but at same time, challenge for development (R&D) towards applications pharmaceutical, food, cosmetics, agrochemical industries. Based its versatility unique capabilities deconvolution metabolite composition matrices, metabolomics an ideal bioanalytical tool could greatly assist accelerate R&D. Among others, or cannabinomics can be applied taxonomy chemovars, discovery assessment new Cannabis-based sources bioactivity medicine, food products, optimization cultivation, aiming improvements yield potency. Although still infancy, it highly foreseen employment advanced will provide insights sector face aforementioned challenges. Within this context, here, current state-of-the-art conceptual aspects presented.
Язык: Английский
Процитировано
84
Analytical and Bioanalytical Chemistry, Год журнала: 2020, Номер 413(10), С. 2599 - 2617
Опубликована: Ноя. 19, 2020
Abstract Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a fast-growing technique for visualization of the spatial distribution small molecular and macromolecular biomolecules in tissue sections. Challenges MALDI-MSI, such as poor sensitivity some classes molecules or limited specificity, instance resulting from presence isobaric resolving power instrument, have encouraged MSI scientific community to improve MALDI-MSI sample preparation workflows with innovations chemistry. Recent developments novel organic MALDI matrices play part improvement image quality expansion application areas MALDI-MSI. This includes rationally designed/synthesized well commercially available whose superior matrix properties comparison common only recently been discovered. Furthermore, on-tissue chemical derivatization (OTCD) processes get more focused attention, because their advantages localization poorly ionizable metabolites their‚ several cases‚ specific review will provide an overview about latest reagents
Язык: Английский
Процитировано
84
Frontiers in Molecular Biosciences, Год журнала: 2021, Номер 8
Опубликована: Сен. 20, 2021
Personalized medicine is probably the most promising area being developed in modern medicine. This approach attempts to optimize therapies and patient care based on individual characteristics. Its success highly depends way characterization of disease its evolution, patient’s classification, follow-up treatment could be optimized. Thus, personalized must combine innovative tools measure, integrate model data. Towards this goal, clinical metabolomics appears as ideally suited obtain relevant information. Indeed, signature brings crucial insight stratify patients according their responses a pathology and/or treatment, provide prognostic diagnostic biomarkers, improve therapeutic outcomes. However, translation from laboratory studies practice remains subsequent challenge. Nuclear magnetic resonance spectroscopy (NMR) mass spectrometry (MS) are two key platforms for measurement metabolome. NMR has several advantages features that essential metabolomics. inherently very robust, reproducible, unbiased, quantitative, informative at structural molecular level, requires little sample preparation reduced data processing. also well adapted large cohorts, multi-sites longitudinal studies. review focus potential context Starting with current status NMR-based level highlighting strengths, weaknesses challenges, article explores how, far initial “opposition” or “competition”, MS have been integrated demonstrated great complementarity, terms classification biomarker identification. Finally, perspective discussion provides into methodological developments significantly raise more resolutive, sensitive accessible tool applications point-of-care diagnosis. Thanks these advances, strong join other analytical currently used settings.
Язык: Английский
Процитировано
77
Metabolites, Год журнала: 2021, Номер 11(3), С. 151 - 151
Опубликована: Март 7, 2021
Dynamic changes in circulating and tissue metabolites lipids occur response to exercise-induced cellular whole-body energy demands maintain metabolic homeostasis. The metabolome lipidome a given biological system provides molecular snapshot of these rapid complex perturbations. application metabolomics lipidomics map the responses an acute bout aerobic/endurance or resistance exercise has dramatically expanded over past decade thanks major analytical advancements, with most exercise-related studies date focused on analyzing human biofluids tissues. Experimental considerations, as well complementary using animal model systems, are warranted help overcome challenges associated large interindividual variability decipher breadth mechanisms underlying health-promoting effects exercise. In this review, we provide guide for researchers regarding techniques experimental workflows commonly used lipidomics. Furthermore, discuss advancements mammalian research utilizing metabolomic lipidomic approaches last decade, highlight key technical considerations remaining knowledge gaps continue expanding landscape biology.
Язык: Английский
Процитировано
71
Clinica Chimica Acta, Год журнала: 2021, Номер 523, С. 231 - 238
Опубликована: Окт. 8, 2021
Язык: Английский
Процитировано
64