Electrophoresis,
Год журнала:
2023,
Номер
44(24), С. 2000 - 2024
Опубликована: Сен. 5, 2023
Abstract
Single‐cell
heterogeneity
in
metabolism,
drug
resistance
and
disease
type
poses
the
need
for
analytical
techniques
single‐cell
analysis.
As
metabolome
provides
closest
view
of
status
quo
cell,
studying
at
resolution
may
unravel
said
heterogeneity.
A
challenge
analysis
is
that
metabolites
cannot
be
amplified,
so
one
needs
to
deal
with
picolitre
volumes
a
wide
range
analyte
concentrations.
Due
high
sensitivity
resolution,
MS
preferred
metabolomics.
Large
numbers
cells
analysed
proper
statistics;
this
requires
high‐throughput
analysis,
hence
automation
workflow.
Significant
advances
(micro)sampling
methods,
CE
ion
mobility
spectrometry
have
been
made,
some
which
applied
analyses.
Microfluidics
has
enabled
an
cell
picking
metabolite
extraction;
image
recognition
automated
identification.
Many
used
data
varying
from
conventional
novel
combinations
advanced
chemometric
approaches.
Steps
set
making
more
findable,
accessible,
interoperable
reusable,
but
significant
opportunities
improvement
remain.
Herein,
workflows
are
discussed,
recommendations
made
based
on
experimental
goal.
Frontiers in Oncology,
Год журнала:
2024,
Номер
14
Опубликована: Апрель 22, 2024
Acute
myeloid
leukemia
(AML)
is
a
complex
and
heterogeneous
group
of
aggressive
hematopoietic
stem
cell
disease.
The
presence
diverse
functionally
distinct
populations
cells
within
the
same
patient’s
bone
marrow
or
blood
poses
significant
challenge
in
diagnosing
treating
AML.
A
substantial
proportion
AML
patients
demonstrate
resistance
to
induction
chemotherapy
grim
prognosis
upon
relapse.
rapid
advance
next
generation
sequencing
technologies,
such
as
single-cell
RNA-sequencing
(scRNA-seq),
has
revolutionized
our
understanding
pathogenesis
by
enabling
high-resolution
interrogation
cellular
heterogeneity
ecosystem,
their
transcriptional
signatures
at
level.
New
studies
have
successfully
characterized
inextricably
intertwined
interactions
among
cells,
immune
microenvironment
contributions
development,
therapeutic
These
findings
deepened
broadened
complexity
AML,
which
are
difficult
detect
with
bulk
RNA-seq.
This
review
encapsulates
burgeoning
body
knowledge
generated
through
scRNA-seq,
providing
novel
insights
discoveries
it
unveiled
biology.
Furthermore,
we
discuss
potential
implications
scRNA-seq
opportunities,
focusing
on
immunotherapy.
Finally,
highlight
current
limitations
future
direction
field.
Advanced Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 4, 2024
Abstract
Metabolic
dysregulation
is
a
key
driver
of
cellular
senescence,
contributing
to
the
progression
systemic
aging.
The
heterogeneity
senescent
cells
and
their
metabolic
shifts
are
complex
unexplored.
A
microfluidic
SlipChip
integrated
with
surface‐enhanced
Raman
spectroscopy
(SERS),
termed
SlipChip‐SERS,
developed
for
single‐cell
metabolism
analysis.
This
SlipChip‐SERS
enables
compartmentalization
single
cells,
parallel
delivery
saponin
nanoparticles
release
intracellular
metabolites
realize
SERS
detection
simple
slipping
operations.
Analysis
different
cancer
cell
lines
using
demonstrated
its
capability
sensitive
multiplexed
profiling
individual
cells.
When
applied
human
primary
fibroblasts
ages,
it
identified
12
differential
metabolites,
spermine
validated
as
potent
inducer
senescence.
Prolonged
exposure
can
induce
classic
senescence
phenotype,
such
increased
senescence‐associated
β‐glactosidase
activity,
elevated
expression
senescence‐related
genes
reduced
LMNB1
levels.
Additionally,
senescence‐inducing
capacity
in
HUVECs
WRL‐68
confirmed,
exogenous
treatment
accumulation
H
2
O
.
Overall,
novel
system
analysis,
revealing
potential
across
multiple
types,
which
may
offer
new
strategies
addressing
ageing
ageing‐related
diseases.
Rapid Communications in Mass Spectrometry,
Год журнала:
2025,
Номер
39(13)
Опубликована: Апрель 13, 2025
ABSTRACT
Single‐cell
metabolomics
is
an
emerging
and
powerful
technology
that
uncovers
intercellular
heterogeneity
reveals
microenvironmental
dynamics
in
both
physiological
pathological
conditions.
This
enables
detailed
observations
of
cellular
interactions,
providing
valuable
insights
into
processes
such
as
aging,
immune
responses,
disease
development.
Despite
significant
advances,
the
need
for
discussions
on
sampling
analytical
methods
single‐cell
continues
to
grow,
with
increasing
focus
selecting
most
suitable
techniques
diverse
research
objectives.
review
addresses
these
challenges
by
exploring
key
strategies
used
metabolomics.
We
three
main
approaches:
capture
isolation
specific
cell
types,
precise
aspiration
individual
cells,
situ
mass
spectrometry
imaging.
These
are
critically
assessed
highlight
achieving
accurate
metabolite
detection
at
level
across
applications.
Sports Medicine - Open,
Год журнала:
2025,
Номер
11(1)
Опубликована: Май 14, 2025
Abstract
Because
of
its
positive
effects
on
the
cardiovascular,
metabolic
and
neurohormonal
systems,
as
well
other
aspects
systemic
physiology,
exercise
is
crucial
to
overall
health.
Traditional
physiology
techniques
that
rely
invasive
procedures
have
limited
our
understanding
molecular
changes
induced
by
exercise.
This
paper
distinguishes
emerging
fields
“
enduromics
”
resistomics
from
sportomics.
Enduromics
concentrate
responses
endurance
resistance
training,
respectively,
in
a
variety
populations,
whereas
sportomics
stresses
study
alterations
athletes
competitive
or
simulated
situations.
These
integrate
biological
systems
with
omics
technology
provide
accurate
insights
into
many
physiological
occur
during
aerobic
anaerobic
methods
make
it
possible
create
individualized
training
plans
maximise
health,
reduce
injury
risk
improve
adherence
identifying
biomarkers
fingerprints.
The
revolutionary
potential
for
athletic
performance
public
health
underscores
need
more
research
across
all
demographics
modalities.
Current Opinion in Biotechnology,
Год журнала:
2024,
Номер
88, С. 103149 - 103149
Опубликована: Май 28, 2024
Single-cell
chemical
and
metabolic
imaging
technologies
provide
unprecedented
insights
into
individual
cell
dynamics,
advancing
our
understanding
of
cellular
processes,
molecular
interactions,
activities.
Advances
in
fluorescence,
Raman,
optoacoustic
(photoacoustic),
or
mass
spectrometry
methods
have
paved
the
way
to
characterize
metabolites,
signaling
molecules,
other
moieties
within
cells.
These
modalities
can
also
lead
single-cell
capabilities
by
targeting
endogenous
contrast
employing
exogenous
generation
techniques,
including
agents
that
target
specific
structure
function.
In
this
review,
we
present
key
developments,
summarize
recent
applications
interrogation
imaging,
illustrate
their
advantages,
limitations,
outlook.
Separations,
Год журнала:
2025,
Номер
12(2), С. 21 - 21
Опубликована: Янв. 24, 2025
Plant
metabolomics,
lipidomics,
ionomics,
fluxomics,
and
peptidomics
are
essential
approaches
for
exploring
how
plants
respond
to
epigenetic,
pathological,
environmental
stimuli
through
comprehensive
chemical
profiling.
Over
the
past
decades,
significant
progress
has
been
made
in
protocols
methodologies
address
challenges
sample
collection
extraction.
Despite
these
advancements,
preparation
remains
intricate,
with
ongoing
debates
about
most
effective
strategies.
This
review
emphasizes
importance
of
clear
research
questions
well-designed
experiments
minimize
complexity,
save
time,
enhance
reproducibility.
It
provides
an
overview
key
steps
fields,
including
harvesting,
drying,
extraction,
data
pre-acquisition
major
analytical
platforms.
By
discussing
best
practices
common
challenges,
this
aims
streamline
methods
promote
more
consistent
reliable
outcomes.
NeoReviews,
Год журнала:
2025,
Номер
26(2), С. e100 - e114
Опубликована: Фев. 1, 2025
The
metabolome
and
lipidome
comprise
the
thousands
of
molecular
compounds
in
an
organism.
Molecular
consist
upstream
metabolic
components
intracellular
reactions
or
byproducts
cellular
pathways.
biochemical
perturbations
are
associated
with
disorders
newborns
infants.
diagnosis
inborn
errors
metabolism
has
relied
on
targeted
metabolomics
for
several
decades.
Newer
approaches
offer
potential
to
identify
novel
biomarkers
common
diseases
newborn
infant.
They
may
also
elucidate
predictive
diagnostic
measures
a
variety
health
trajectories.
Here,
we
review
relevance
highlight
challenges
opportunities
future
investigations.
