Epithelial-to-mesenchymal
transition
(EMT)
is
a
crucial
cellular
process
for
embryogenesis,
wound
healing,
and
cancer
progression.
It
involves
shift
in
cell
interactions,
leading
to
the
detachment
of
epithelial
cells
activation
gene
programs
promoting
mesenchymal
state.
EMT
plays
significant
role
metastasis
triggering
tumor
initiation
stemness,
activates
metastatic
cascades
resulting
resistance
therapy.
Moreover,
reversal
contributes
formation
lesions.
Metastasis
still
needs
be
better
understood
functionally
its
major
but
complex
steps
migration,
invasion,
intravasation,
dissemination,
which
establishment
minimal
residual
disease
(MRD),
extravasation,
successful
seeding
growth
lesions
at
microenvironmentally
heterogeneous
sites.
Therefore,
current
review
article
intends
present,
discuss
comprehensively,
status
quo
experimental
models
able
investigate
vitro
vivo,
researchers
planning
enter
field.
We
emphasize
various
methods
understand
function
metastasis,
including
diverse
invasion
matrix
degradation
assays,
microfluidics,
3D
co-culture
models,
spheroids,
organoids,
or
latest
spatial
imaging
analyze
compartments.
In
vivo
such
as
chorionallantoic
membrane
(CAM)
assay,
line-derived
patient-derived
xenografts,
syngeneic,
genetically
modified,
humanized
mice,
are
presented
promising
arsenal
tools
site
specific
treatment
response.
Furthermore,
we
give
brief
overview
on
detecting
dissemination
MRD
carcinomas,
highlighting
significance
tracking
course
response
treatment.
Enhanced
lineage
tools,
dynamic
imaging,
therapeutically
useful
powerful
preclinical
may
reveal
functional
interdependencies
between
EMT.
Future
directions
discussed
light
emerging
views
biology,
diagnosis,
metastasis.
Rapid Communications in Mass Spectrometry,
Год журнала:
2025,
Номер
39(13)
Опубликована: Апрель 13, 2025
ABSTRACT
Single‐cell
metabolomics
is
an
emerging
and
powerful
technology
that
uncovers
intercellular
heterogeneity
reveals
microenvironmental
dynamics
in
both
physiological
pathological
conditions.
This
enables
detailed
observations
of
cellular
interactions,
providing
valuable
insights
into
processes
such
as
aging,
immune
responses,
disease
development.
Despite
significant
advances,
the
need
for
discussions
on
sampling
analytical
methods
single‐cell
continues
to
grow,
with
increasing
focus
selecting
most
suitable
techniques
diverse
research
objectives.
review
addresses
these
challenges
by
exploring
key
strategies
used
metabolomics.
We
three
main
approaches:
capture
isolation
specific
cell
types,
precise
aspiration
individual
cells,
situ
mass
spectrometry
imaging.
These
are
critically
assessed
highlight
achieving
accurate
metabolite
detection
at
level
across
applications.
Sports Medicine - Open,
Год журнала:
2025,
Номер
11(1)
Опубликована: Май 14, 2025
Abstract
Because
of
its
positive
effects
on
the
cardiovascular,
metabolic
and
neurohormonal
systems,
as
well
other
aspects
systemic
physiology,
exercise
is
crucial
to
overall
health.
Traditional
physiology
techniques
that
rely
invasive
procedures
have
limited
our
understanding
molecular
changes
induced
by
exercise.
This
paper
distinguishes
emerging
fields
“
enduromics
”
resistomics
from
sportomics.
Enduromics
concentrate
responses
endurance
resistance
training,
respectively,
in
a
variety
populations,
whereas
sportomics
stresses
study
alterations
athletes
competitive
or
simulated
situations.
These
integrate
biological
systems
with
omics
technology
provide
accurate
insights
into
many
physiological
occur
during
aerobic
anaerobic
methods
make
it
possible
create
individualized
training
plans
maximise
health,
reduce
injury
risk
improve
adherence
identifying
biomarkers
fingerprints.
The
revolutionary
potential
for
athletic
performance
public
health
underscores
need
more
research
across
all
demographics
modalities.
Acta Biomedica Scientifica (East Siberian Biomedical Journal),
Год журнала:
2025,
Номер
10(2), С. 33 - 47
Опубликована: Май 19, 2025
Background.
Teraher
tz
r
adiation
(THz),
which
occupies
the
frequency
range
from
0.1
to
10
THz,
has
been
a
topic
of
limited
research
for
long
time
due
difficulty
in
creating
sources
and
detecting
it.
Recent
advancements
semiconductor
nanotechnology,
however,
have
led
development
THz
technologies
areas
such
as
communications,
medicine,
safety.
Nevertheless,
there
are
concerns
about
potential
health
environmental
effects
these
technologies.
The
aim.
Summariz
e
current
state
resear
ch
fi
c
ellular
eff
ts
arising
fr
om
exp
osure
t
o
TH
z.
Special
att
ention
is
paid
use
ffi
technologies,
particular
metabolomics,
proteomics,
transcriptomics
study
on
living
systems.
review
also
aims
analyze
key
patterns
biological
caused
by
assess
prospects
further
application
biomedical
biotechnological
direction
features
experiment
organization
infl
THz.
To
write
review,
search
scientifi
publications
was
carried
out
using
PubMed,
Google
Scholar,
Scopus,
IEEE
Xplore
period
2000
2024.
Discussion.
studies
hav
shown
non-thermal
ells,
including
genotoxicity
changes
gene
expr
ession.
Ho
wever,
results
vary
depending
con
ditions
ell
yp
es
used
.
Most
b
een
conducted
vitro
various
cell
lines,
depend
radiation
parameters
wavelength
intensity.
For
epithelial
cells
fibroblasts,
cytotoxicity
generally
low
at
1
although
genotoxic
cannot
be
ruled
out.
reduce
DNA
methylation
tumor
cells,
could
useful
diagnosis.
Omics
helping
molecular
mechanisms
underlying
effects,
but
standardizing
methods
crucial
accurately
differentiate
between
thermal
mechanisms.
Conclusion.
T
he
emphasiz
importance
its
impact
However,
available
data
dispersed.
comprehend
detailed
experimental
investigations
required,
metabolomics
approaches
analyzing
biochemical
responses
radiation.
Epithelial-to-mesenchymal
transition
(EMT)
is
a
crucial
cellular
process
for
embryogenesis,
wound
healing,
and
cancer
progression.
It
involves
shift
in
cell
interactions,
leading
to
the
detachment
of
epithelial
cells
activation
gene
programs
promoting
mesenchymal
state.
EMT
plays
significant
role
metastasis
triggering
tumor
initiation
stemness,
activates
metastatic
cascades
resulting
resistance
therapy.
Moreover,
reversal
contributes
formation
lesions.
Metastasis
still
needs
be
better
understood
functionally
its
major
but
complex
steps
migration,
invasion,
intravasation,
dissemination,
which
establishment
minimal
residual
disease
(MRD),
extravasation,
successful
seeding
growth
lesions
at
microenvironmentally
heterogeneous
sites.
Therefore,
current
review
article
intends
present,
discuss
comprehensively,
status
quo
experimental
models
able
investigate
vitro
vivo,
researchers
planning
enter
field.
We
emphasize
various
methods
understand
function
metastasis,
including
diverse
invasion
matrix
degradation
assays,
microfluidics,
3D
co-culture
models,
spheroids,
organoids,
or
latest
spatial
imaging
analyze
compartments.
In
vivo
such
as
chorionallantoic
membrane
(CAM)
assay,
line-derived
patient-derived
xenografts,
syngeneic,
genetically
modified,
humanized
mice,
are
presented
promising
arsenal
tools
site
specific
treatment
response.
Furthermore,
we
give
brief
overview
on
detecting
dissemination
MRD
carcinomas,
highlighting
significance
tracking
course
response
treatment.
Enhanced
lineage
tools,
dynamic
imaging,
therapeutically
useful
powerful
preclinical
may
reveal
functional
interdependencies
between
EMT.
Future
directions
discussed
light
emerging
views
biology,
diagnosis,
metastasis.
