Aging, Год журнала: 2023, Номер unknown
Опубликована: Авг. 11, 2023
Язык: Английский
Investigative Ophthalmology & Visual Science, Год журнала: 2024, Номер 65(6), С. 1 - 1
Опубликована: Июнь 3, 2024
Purpose: Loss of function the lacrimal gland (LG), which produces aqueous tear film, is implicated in age-related dry eye. To better understand this deterioration, we evaluated changes lipid metabolism and inflammation LGs from an aging model. Methods: LG sections female C57BL/6J mice different ages (young, 2–3 months; intermediate, 10–14 old, ≥24 months) were stained with Oil Red-O or Toluidine blue to detect lipids. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis western blotting lysates determined differences expression genes proteins related metabolism. A photobleaching protocol quench autofluorescence was used evaluate immunofluorescence associated NPC1, NPC2, CTSL, macrophages (F4/80, CD11b) age using confocal fluorescence microscopy. Results: Old showed increased lipids prominent basal aggregates acinar cells extra-acinar sites. gene Npc1, Npc2, Lipa, Mcoln2, encoding involved metabolism, age. NPC1 also significantly old by blotting. In photobleached sections, microscopy imaging CTSL age-associated enrichment labeled F4/80. Although mononuclear detectable at all ages, novel multinucleate macrophage population containing enriched F4/80 some CD11b Conclusions: Lipid-metabolizing F4/80-positive multinucleated are adjacent sites deposition acini.
Язык: Английский
Процитировано
2
Investigative Ophthalmology & Visual Science, Год журнала: 2023, Номер 64(11), С. 7 - 7
Опубликована: Авг. 4, 2023
Aged C57BL/6J (B6) mice have increased levels of cathepsin S, and aged S (Ctss-/-) knockout are resistant to age-related dry eye. This study investigated the effects inhibition on eye disease.
Язык: Английский
Процитировано
6
International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(5), С. 4897 - 4897
Опубликована: Март 3, 2023
Aging is associated with inflammation and oxidative stress in the lacrimal gland (LG). We investigated if heterochronic parabiosis of mice could modulate age-related LG alterations. In both males females, there were significant increases total immune infiltration isochronic aged LGs compared to that young LGs. Male significantly more infiltrated male While females had inflammatory B-cell-related transcripts levels LGs, a greater fold expression some these than males. Through flow cytometry, specific subsets B cells increased those Our results indicate serum soluble factors from not enough reverse infiltrating tissues sex-related differences treatment. This suggests changes microenvironment/architecture participate perpetuating inflammation, which reversible by exposure youthful systemic factors. contrast, worse their counterparts, suggesting can enhance host. Therapies aim at improving cellular health may have stronger impact on parabiosis.
Язык: Английский
Процитировано
3
The Ocular Surface, Год журнала: 2023, Номер 30, С. 57 - 72
Опубликована: Июль 27, 2023
Язык: Английский
Процитировано
2
Aging, Год журнала: 2023, Номер unknown
Опубликована: Авг. 11, 2023
Язык: Английский
Процитировано
0