The ISME Journal,
Год журнала:
2020,
Номер
15(3), С. 848 - 861
Опубликована: Ноя. 4, 2020
Abstract
Asgard
is
a
recently
discovered
archaeal
superphylum,
closely
linked
to
the
emergence
of
eukaryotes.
Among
archaea,
Lokiarchaeota
are
abundant
in
marine
sediments,
but
their
situ
activities
largely
unknown
except
for
Candidatus
‘Prometheoarchaeum
syntrophicum’.
Here,
we
tracked
activity
incubations
with
Helgoland
mud
area
sediments
(North
Sea)
by
stable
isotope
probing
(SIP)
organic
polymers,
13C-labelled
inorganic
carbon,
fermentation
intermediates
and
proteins.
Within
active
detected
members
class
Loki-3,
which
appeared
mixotrophically
participate
degradation
lignin
humic
acids
while
assimilating
CO2,
or
heterotrophically
used
lactate.
In
contrast,
Loki-2
utilized
protein
degraded
bacterial
biomass
formed
incubations.
Metagenomic
analysis
revealed
pathways
lactate
degradation,
involvement
aromatic
compound
less
globally
distributed
instead
rely
on
degradation.
We
conclude
that
Lokiarchaeotal
subgroups
vary
metabolic
capabilities
despite
overlaps
genomic
equipment,
suggest
these
occupy
different
ecologic
niches
sediments.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Июль 31, 2024
Abstract
The
roles
of
Asgard
archaea
in
eukaryogenesis
and
marine
biogeochemical
cycles
are
well
studied,
yet
their
contributions
soil
ecosystems
remain
unknown.
Of
particular
interest
archaeal
to
methane
cycling
wetland
soils.
To
investigate
this,
we
reconstructed
two
complete
genomes
for
soil-associated
Atabeyarchaeia,
a
new
lineage,
genome
Freyarchaeia,
predicted
metabolism
situ.
Metatranscriptomics
reveals
expression
genes
[NiFe]-hydrogenases,
pyruvate
oxidation
carbon
fixation
via
the
Wood-Ljungdahl
pathway.
Also
expressed
encoding
enzymes
amino
acid
metabolism,
anaerobic
aldehyde
oxidation,
hydrogen
peroxide
detoxification
carbohydrate
breakdown
acetate
formate.
Overall,
include
non-methanogenic
acetogens,
highlighting
potential
role
terrestrial
environments.
Frontiers in Microbiology,
Год журнала:
2020,
Номер
11
Опубликована: Авг. 28, 2020
Archaea
are
diverse
and
ubiquitous
prokaryotes
present
in
both
extreme
moderate
environments.
Estuaries,
serving
as
links
between
the
land
ocean,
harbor
numerous
microbes
that
relatively
highly
active
because
of
massive
terrigenous
input
nutrients.
account
for
a
considerable
portion
estuarine
microbial
community.
They
play
key
roles
biogeochemical
cycles.
Ammonia-oxidizing
archaea
(AOA)
an
abundant
aquatic
archaeal
group
estuaries,
greatly
contributing
ammonia
oxidation.
Bathyarchaeota
sediments,
they
may
involve
sedimentary
organic
matter
degradation,
acetogenesis,
and,
potentially,
methane
metabolism,
based
on
genomics.
Other
groups
also
commonly
detected
estuaries
worldwide.
include
Euryarchaeota,
members
DPANN
Asgard
archaea.
Based
biodiversity
surveys
16S
rRNA
gene
some
functional
genes,
distribution
abundance
driven
by
physicochemical
factors,
such
salinity
oxygen
concentration.
Currently,
increasing
amount
genomic
information
is
becoming
available
advances
sequencing
technologies,
especially
AOA
Bathyarchaeota,
leading
to
better
understanding
their
functions
environmental
adaptations.
Here,
we
summarized
current
knowledge
community
composition
major
focusing
Bathyarchaeota.
We
highlighted
unique
features
potential
adaptation
strategies
archaea,
pointing
out
unknowns
field
scope
future
research.
The ISME Journal,
Год журнала:
2020,
Номер
15(3), С. 848 - 861
Опубликована: Ноя. 4, 2020
Abstract
Asgard
is
a
recently
discovered
archaeal
superphylum,
closely
linked
to
the
emergence
of
eukaryotes.
Among
archaea,
Lokiarchaeota
are
abundant
in
marine
sediments,
but
their
situ
activities
largely
unknown
except
for
Candidatus
‘Prometheoarchaeum
syntrophicum’.
Here,
we
tracked
activity
incubations
with
Helgoland
mud
area
sediments
(North
Sea)
by
stable
isotope
probing
(SIP)
organic
polymers,
13C-labelled
inorganic
carbon,
fermentation
intermediates
and
proteins.
Within
active
detected
members
class
Loki-3,
which
appeared
mixotrophically
participate
degradation
lignin
humic
acids
while
assimilating
CO2,
or
heterotrophically
used
lactate.
In
contrast,
Loki-2
utilized
protein
degraded
bacterial
biomass
formed
incubations.
Metagenomic
analysis
revealed
pathways
lactate
degradation,
involvement
aromatic
compound
less
globally
distributed
instead
rely
on
degradation.
We
conclude
that
Lokiarchaeotal
subgroups
vary
metabolic
capabilities
despite
overlaps
genomic
equipment,
suggest
these
occupy
different
ecologic
niches
sediments.
