Boosting wheat functional genomics via an indexed EMS mutant library of KN9204

Plant Communications, Год журнала: 2023, Номер 4(4), С. 100593 - 100593

Опубликована: Март 21, 2023

A better understanding of wheat functional genomics can improve targeted breeding for agronomic traits and environmental adaptation. However, the lack gene-indexed mutants low transformation efficiency limit in-depth gene studies genetic manipulation breeding. In this study, we created a library KN9204, popular variety in northern China, with reference genome, transcriptome, epigenome different tissues, using ethyl methyl sulfonate (EMS) mutagenesis. This contains vast developmental diversity critical tissues transition stages. Exome capture sequencing 2090 mutant lines KN9204 genome-designed probes revealed that 98.79% coding genes had mutations, each line an average 1383 EMS-type SNPs. We identified new allelic variations crucial trait-related such as Rht-D1, Q, TaTB1, WFZP. tested 100 severe mutations 80 NAC transcription factors (TFs) under drought salinity stress 13 altered sensitivity. Further analysis three transcriptome chromatin accessibility data hundreds direct targets patterns salt or stress, including SNAC1, DREB2B, CML16, ZFP182, known to respond abiotic stress. Thus, have generated indexed EMS facilitate research offer resources wheat.

Язык: Английский

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Wheat Pm55 alleles exhibit distinct interactions with an inhibitor to cause different powdery mildew resistance

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Янв. 13, 2024

Abstract Powdery mildew poses a significant threat to wheat crops worldwide, emphasizing the need for durable disease control strategies. The wheat- Dasypyrum villosum T5AL·5 V#4 S and T5DL·5 translocation lines carrying powdery resistant gene Pm55 shows developmental-stage tissue-specific resistance, whereas V#5 line Pm5V confers resistance at all stages. Here, we clone , reveal that they are allelic renamed as Pm55a Pm55b respectively. two alleles encode coiled-coil, nucleotide-binding site-leucine-rich repeat (CNL) proteins, conferring broad-spectrum mildew. However, interact differently with linked inhibitor gene, SuPm55 cause different Notably, unrelated CNL inactivation of significantly reduces plant fitness. Combining / in does not result allele suppression or yield penalty. Our results provide only insights into wheat, but also strategy breeding resistance.

Язык: Английский

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Deciphering the Transcriptional Regulatory Network Governing Starch and Storage Protein Biosynthesis in Wheat for Breeding Improvement

Advanced Science, Год журнала: 2024, Номер 11(33)

Опубликована: Июнь 28, 2024

Starch and seed storage protein (SSP) composition profoundly impact wheat grain yield quality. To unveil regulatory mechanisms governing their biosynthesis, transcriptome, epigenome profiling is conducted across key endosperm developmental stages, revealing that chromatin accessibility, H3K27ac, H3K27me3 collectively regulate SSP starch genes with varying impact. Population transcriptome phenotype analyses highlight accessible promoter regions' crucial role as a genetic variation resource, influencing quality in core collection of accessions. Integration time-serial RNA-seq ATAC-seq enables the construction hierarchical transcriptional network identifying 42 high-confidence novel candidates. These candidates exhibit overlap regions associated size traits, functional significance validated through expression-phenotype association analysis among accessions loss-of-function mutants. Functional abscisic acid insensitive 3-A1 (TaABI3-A1) genome editing knock-out lines demonstrates its promoting accumulation while repressing biosynthesis regulation. Excellent TaABI3-A1

Язык: Английский

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WRKY Transcription Factor Responses and Tolerance to Abiotic Stresses in Plants

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(13), С. 6845 - 6845

Опубликована: Июнь 21, 2024

Plants are subjected to abiotic stresses throughout their developmental period. Abiotic include drought, salt, heat, cold, heavy metals, nutritional elements, and oxidative stresses. Improving plant responses various environmental is critical for survival perpetuation. WRKY transcription factors have special structures (WRKY structural domains), which enable the different transcriptional regulatory functions. can not only regulate stress growth development by regulating phytohormone signalling pathways but also promote or suppress expression of downstream genes binding W-box [TGACCA/TGACCT] in promoters target genes. In addition, interact with other families defence self-regulate recognising W-boxes own However, recent years, research reviews on roles higher plants been scarce shallow. this review, we focus structure classification factors, as well identification molecular mechanisms involved response stresses, improve tolerance ability under stress, look forward future directions, a view providing theoretical support genetic improvement crop tolerance.

Язык: Английский

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WRKY Transcription Factor Responses and Tolerance to Abiotic Stresses in Plants

Опубликована: Июнь 3, 2024

Plants are subjected to abiotic stresses throughout their developmental period. Abiotic include drought, salt, heat, cold, heavy metals, nutritional element and oxidative stresses. Improving plant response various environmental is critical for survival perpetuation. The WRKY transcription factors have special structure (WRKY structural domains), which enable different transcriptional regulatory functions. can not only regulate growth development by regulating phytohormone signalling pathways, but also promote or suppress the expression of downstream genes binding W-box [TGACCA/TGACCT] in promoters target genes. In addition, interact with other families defence responses stresses, self-regulate recognizing W-boxes own However, recent years, research reviews on roles higher plants scarce shallow. this review, we focus classification factors, as well identification molecular mechanisms involved improve tolerance ability stress look forward future directions, a view providing theoretical support genetic improvement crop tolerance.

Язык: Английский

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Complex genetic architecture underlying the plasticity of maize agronomic traits

Plant Communications, Год журнала: 2023, Номер 4(3), С. 100473 - 100473

Опубликована: Янв. 14, 2023

Phenotypic plasticity is the ability of a given genotype to produce multiple phenotypes in response changing environmental conditions. Understanding genetic basis phenotypic and establishing predictive model highly relevant future agriculture under climate. Here we report findings on for 23 complex traits using diverse maize population planted at five sites with distinct We found that latitude-related factors were main drivers across-site variation flowering time but not plant architecture or yield traits. For traits, detected 109 quantitative trait loci (QTLs), 29 mean values, 66 plasticity, 14 both parameters, 80% QTLs interacted latitude. The effects several changed magnitude sign, driving plasticity. experimentally validated one plastic gene, ZmTPS14.1, whose effect was likely mediated by compensation ZmSPL6 from downstream pathway. By integrating diversity, variation, their interaction into joint model, could provide site-specific predictions increased accuracy as much 9.9%, 2.2%, 2.6% days tassel, height, ear weight, respectively. This study revealed involving alleles, pleiotropy, genotype-by-environment underlies It provides novel insights dynamic agronomic environments, paving practical way toward precision agriculture.

Язык: Английский

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Deciphering spike architecture formation towards yield improvement in wheat

Journal of genetics and genomics/Journal of Genetics and Genomics, Год журнала: 2023, Номер 50(11), С. 835 - 845

Опубликована: Март 11, 2023

Wheat is the most widely grown crop globally, providing 20% of daily consumed calories and protein content around world. With growing global population frequent occurrence extreme weather caused by climate change, ensuring adequate wheat production essential for food security. The architecture inflorescence plays a crucial role in determining grain number size, which key trait improving yield. Recent advances genomics gene cloning techniques have improved our understanding spike development its applications breeding practices. Here, we summarize genetic regulation network governing formation, strategies used identifying studying factors affecting architecture, progress made applications. Additionally, highlight future directions that will aid regulatory mechanistic study determination targeted yield improvement.

Язык: Английский

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CRISPR-mediated acceleration of wheat improvement: advances and perspectives

Journal of genetics and genomics/Journal of Genetics and Genomics, Год журнала: 2023, Номер 50(11), С. 815 - 834

Опубликована: Сен. 21, 2023

Common wheat (Triticum aestivum) is one of the most widely cultivated and consumed crops globally. In face limited arable land climate changes, it a great challenge to maintain current increase future production. Enhancing agronomic traits in by introducing mutations across all three homoeologous copies each gene has proven be difficult task due its large genome with high repetition. However, clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease (Cas) editing technologies offer powerful means precisely manipulating genomes crop species, thereby opening up new possibilities for biotechnology breeding. this review, we first focus on development optimization CRISPR-based tools wheat, emphasizing recent breakthroughs precise multiplex editing. We then describe general procedure highlight different methods deliver reagents into cells. Furthermore, summarize applications advancements CRISPR/Cas improvement. Lastly, discuss remaining challenges specific prospects.

Язык: Английский

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Two functional CC‐NBS‐LRR proteins from rye chromosome 6RS confer differential age‐related powdery mildew resistance to wheat

Plant Biotechnology Journal, Год журнала: 2023, Номер 22(1), С. 66 - 81

Опубликована: Авг. 24, 2023

Summary Rye ( Secale cereale ), a valuable relative of wheat, contains abundant powdery mildew resistance Pm ) genes. Using physical mapping, transcriptome sequencing, barley stripe mosaic virus‐induced gene silencing, ethyl methane sulfonate mutagenesis, and stable transformation, we isolated validated two coiled‐coil, nucleotide‐binding site leucine‐rich repeat (CC‐NBS‐LRR) alleles, PmTR1 PmTR3 , located on rye chromosome 6RS from different triticale lines. confers age‐related starting the three‐leaf stage, whereas its allele, typical all‐stage resistance, which may be associated with their differential expression patterns. Overexpression in Nicotiana benthamiana showed that CC, CC‐NBS, CC‐LRR fragments PMTR1 induce cell death, PMTR3 CC full‐length perform this function. Luciferase complementation imaging pull‐down assays revealed distinct interaction activities between NBS fragments. Our study elucidates novel rye‐derived genes derivative germplasm resources provides insights into mechanism can aid improvement against wheat mildew.

Язык: Английский

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Embracing plant plasticity or robustness as a means of ensuring food security

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 7, 2025

The dual challenges of global population explosion and environmental deterioration represent major hurdles for 21st Century agriculture culminating in an unprecedented demand food security. In this Review, we revisit historical concepts plasticity canalization before integrating them with contemporary studies genotype-environment interactions (G×E) that are currently being carried out at the genome-wide level. doing so address both fundamental questions regarding G×E potential strategies to best secure yields current future climate scenarios. Breeding adaptive crop cultivars under changing scenario is anything but easy. Here, authors review their integration genotype-environmental objective facilitate breeding.

Язык: Английский

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