Current Opinion in Systems Biology, Год журнала: 2023, Номер 37, С. 100490 - 100490
Опубликована: Ноя. 23, 2023
Язык: Английский
Life Sciences, Год журнала: 2024, Номер 361, С. 123300 - 123300
Опубликована: Дек. 5, 2024
Язык: Английский
Процитировано
2
BioTechniques, Год журнала: 2024, Номер 76(5), С. 207 - 219
Опубликована: Апрель 4, 2024
In the absence of a DNA template, ab initio production long double-stranded molecules predefined sequences is particularly challenging. The synthesis step remains bottleneck for many applications such as functional assessment ancestral genes, analysis alternative splicing or DNA-based data storage. this report we propose fully in vitro protocol to generate very starting from commercially available short blocks less than 3 days using Golden Gate assembly. This innovative application allowed us streamline process produce 24 kb-long molecule storing part Declaration Rights Man and Citizen 1789 . produced can be readily cloned into suitable host/vector system amplification selection.
Язык: Английский
Процитировано
1
BioDesign Research, Год журнала: 2024, Номер 6
Опубликована: Янв. 1, 2024
Messenger RNA (mRNA) therapeutics hold great potential in the prevention and treatment of many diseases owing to several unique advantages. Delivery mRNA into target cells is a critical step therapy. Efficient safe delivery systems remain an urgent need. Here, we provide overview current applications protein nanocages (PNCs), which include different types PNCs, such as viral capsids, nonviral artificial delivery. PNCs have features uniform size, controllable assembly, modifiable inner outer surfaces, good biocompatibility, biodegradability, making them ideal candidates for In this review, properties, loading strategies, outcomes each tested PNC are introduced. The challenges faced by PNC-based carriers discussed. We also share our perspectives on possible strategies address these challenges, emphasizing opportunities brought emerging technologies disciplinary convergence.
Язык: Английский
Процитировано
1
Frontiers in Bioengineering and Biotechnology, Год журнала: 2023, Номер 11
Опубликована: Май 4, 2023
Synthetic biology combines the disciplines of biology, chemistry, information science, and engineering, has multiple applications in biomedicine, bioenergy, environmental studies, other fields. genomics is an important area synthetic mainly includes genome design, synthesis, assembly, transfer. Genome transfer technology played enormous role development genomics, allowing natural or genomes into cellular environments where can be easily modified. A more comprehensive understanding help to extend its microorganisms. Here, we summarize three host platforms for microbial transfer, review recent advances that have been made technology, discuss obstacles prospects
Язык: Английский
Процитировано
2
Current Opinion in Systems Biology, Год журнала: 2023, Номер 37, С. 100490 - 100490
Опубликована: Ноя. 23, 2023
Язык: Английский
Процитировано
1