Targeted degradation of endogenous condensation-prone proteins improves crop performance DOI Creative Commons
Ming Luo, Qing Wen, Sitao Zhu

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 15, 2024

Abstract Effective methods, such as CRISPR and RNA interference, exist for modulating gene expression at DNA levels, but approaches directly modifying endogenous proteins remain lacking in plants. Here, we develop a targeted condensation-prone-protein degradation (TCD) strategy to eliminate proteins, particularly those prone condensation. We identify an E3 ligase, E3TCD1, that degrades itself selectively targets other when fused them. In rice, transgenic E3TCD1 fusions with Teosinte branched 1 Early flowering 3 (OsELF3) modulate tiller numbers times, respectively. The TCD system is also controllable. Using the Pro TBF1 -uORFs control cassette, can conditionally degrade negative defense regulator OsELF3 upon pathogen invasion, enhancing rice resistance without interfering time. This method, unlike animal-targeting strategies, does not rely on small molecules, antibodies, or genetic knock-ins, showing promise therapeutic avenue optimizing crop performance potentially addressing human diseases.

Язык: Английский

核糖体介导的翻译调控与植物环境温度适应 DOI
Xiaofan Zhang, Zhen Wang, Can Zhou

и другие.

Chinese Science Bulletin (Chinese Version), Год журнала: 2025, Номер 70(7), С. 807 - 815

Опубликована: Фев. 8, 2025

Процитировано

1
Genetically-encoded targeted protein degradation technology to remove endogenous condensation-prone proteins and improve crop performance DOI Creative Commons
Ming Luo, Sitao Zhu,

Hua Dang

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 29, 2025

Effective modulation of gene expression in plants is achievable through tools like CRISPR and RNA interference, yet methods for directly modifying endogenous proteins remain lacking. Here, we identify the E3 ubiquitin ligase E3TCD1 develope a Targeted Condensation-prone-protein Degradation (TCD) strategy. The X-E3TCD1 fusion protein acts as genetically engineered degrader, selectively targeting prone to condensation. For example, transgenic with Teosinte branched 1 (TB1) degrades native TB1 protein, resulting increased tiller numbers rice. Additionally, conditional degradation negative defense regulator Early Flowering 3 via pathogen-responsive ProTBF1-uORFsTBF1 cassette enhances rice blast resistance without affecting flowering time absence pathogen. Unlike prevailing targeted strategies, TCD system does not rely on small molecules, antibodies, or genetic knock-in tags, demonstrating its promise transgene-based approach optimizing crop performance.

Язык: Английский

Процитировано

0
GENOME EDITING FOR IMPROVING DISEASE RESISTANCE IN RICE (Oryza sativa L.) - SCOPE, ACHIEVEMENTS, LIMITATIONS AND FUTURE NEEDS DOI
Shobica Priya Ramasamy,

Sakthi Ambothi Rathnasamy,

Sudha Manickam

и другие.

Physiological and Molecular Plant Pathology, Год журнала: 2025, Номер unknown, С. 102640 - 102640

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Engineering crop performance with upstream open reading frames DOI

Rui Mou,

Ruixia Niu,

Rong‐Cai Yang

и другие.

Trends in Plant Science, Год журнала: 2024, Номер 30(3), С. 311 - 323

Опубликована: Окт. 30, 2024

Язык: Английский

Процитировано

4
Regulation of potato late blight resistance genes by different promoters and translation regulators DOI Creative Commons

N Wang,

Jiahui Nie,

Jiahui Wu

и другие.

Horticulture Advances, Год журнала: 2025, Номер 3(1)

Опубликована: Апрель 9, 2025

Abstract Late blight, caused by Phytophthora infestans , is one of the most devastative global plant threats. Pyramiding multiple resistance genes ( R genes) was initially considered as a promising strategy for breeding broad-spectrum late blight-resistance potato varieties. However, stacking may induce detrimental effects on health. Fine-tuning expression critical their effective in breeding. This study confirms that low-level individual blight like RB R8 and Vnt1.1 controlled CaMV 35S promoter, does not trigger cell death (CD). co-expression these Nicotiana benthamiana leaves induces CD. Plant upstream open reading frames (uORFs) attenuate translation downstream main ORF. We demonstrates N. frame (NbuORF) Solanum tuberosum L. (StuORF) completely suppress green fluorescent protein (GFP) translation, whereas Arabidopsis thaliana (AtuORFs) only partially inhibit GFP leaves. Simultaneous AtuORFs or StuORF under control either pathogen-inducible vetispiradiene synthase gene 3 (PVS3) promoter leads to inhibition Transgenic potatoes with 35S::RB exhibit superior compared PVS3::RB transgenic lines. Plants expressing 35S::StuORF-RB show increased resistance, although slightly lower than plants. 35S::AtuORFs-RB PVS3::AtuORFs-RB are susceptible P. suggesting These findings highlight potential different uORF combinations finely tuning expression, which optimize balance between normal growth.

Язык: Английский

Процитировано

0
Trans-crop applications of atypical R genes for multipathogen resistance DOI
Peng Sun, Xinyu Han, Ricky J. Milne

и другие.

Trends in Plant Science, Год журнала: 2024, Номер 29(10), С. 1103 - 1112

Опубликована: Май 28, 2024

Язык: Английский

Процитировано

2
Targeted degradation of endogenous condensation-prone proteins improves crop performance DOI Creative Commons
Ming Luo, Qing Wen, Sitao Zhu

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 15, 2024

Abstract Effective methods, such as CRISPR and RNA interference, exist for modulating gene expression at DNA levels, but approaches directly modifying endogenous proteins remain lacking in plants. Here, we develop a targeted condensation-prone-protein degradation (TCD) strategy to eliminate proteins, particularly those prone condensation. We identify an E3 ligase, E3TCD1, that degrades itself selectively targets other when fused them. In rice, transgenic E3TCD1 fusions with Teosinte branched 1 Early flowering 3 (OsELF3) modulate tiller numbers times, respectively. The TCD system is also controllable. Using the Pro TBF1 -uORFs control cassette, can conditionally degrade negative defense regulator OsELF3 upon pathogen invasion, enhancing rice resistance without interfering time. This method, unlike animal-targeting strategies, does not rely on small molecules, antibodies, or genetic knock-ins, showing promise therapeutic avenue optimizing crop performance potentially addressing human diseases.

Язык: Английский

Процитировано

0