A genome-wide association study identified candidate genes associated with egg quality traits in Muscovy duck

BMC Genomics, Год журнала: 2025, Номер 26(1)

Опубликована: Апрель 29, 2025

Egg quality directly determines embryo development in meat-type poultry. However, it is difficult to select the egg of Muscovy duck. The genes and SNPs associated with screened by GWAS can be used for molecular breeding accelerate progress selection 295 ducks were whole genome sequencing, a total 6,131,623 obtained further analysis. heritability ranged from 0.01 0.41, which weight (EW) was 0.19, albumen (AW) 0.16 yolk (YW) 0.27. genetic correlation EW AW, YW, eggshell thickness (EST) strength (ESS) 0.65, 0.51, 0.74, respectively. Phenotypic correlations between - 0.13 0.17. A 68 significantly located within PSMG4, SLC22A23, DNAH5, FABP6, ADAMST17, IGF1R, NTRK3, SCAI. linkage disequilibrium (LD) analysis identified 2_75684453_C > G, 2_76305509_A 2_76350118_T A, 11_3834664_C T, 11_4339778_C 11_8079686_C T as tagSNPs represent significant SNPs. Fifty YW XKR6, DNAJC24, SNCB, UNC5A, MAD1L1, NOTCH1, WDR7. 14_9186714_C 14_9199818_A 15_5452098_C 18_9038052_C selected tagSNPs. Fifty-four height LIN9 NID1. SNP 3_17718980_A G tagSNP. CLPX, EPHA5, ZBTB44, NOL6, UBAP1. 25_1996726_A C 25_2078328_A Genes enriched positive regulation BMP signaling pathway GO enrichment biological processes. KEGG suggested that Axon guidance, Endocrine resistance, Progesterone-mediated oocyte maturation. Some may useful quality. RNF423, RNF220, WDR7, NTRK3 candidate traits

Язык: Английский

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Arkadia and Ark2c Promote Substrate Ubiquitylation with Multiple E2 Enzymes

Опубликована: Янв. 1, 2025

Язык: Английский

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Arkadia and Ark2C promote substrate ubiquitylation with multiple E2 enzymes

Journal of Molecular Biology, Год журнала: 2025, Номер unknown, С. 169259 - 169259

Опубликована: Май 1, 2025

Ubiquitylation is a critical post-translational modification that required for cell vitality. Attachment of ubiquitin complex, with the fate ubiquitylated proteins determined by length attached chains and nature linkage used to build chains. Many E3 ligases attach different types substrate in context specific manner, but molecular details how specify poorly understood. Arkadia/RNF111 large RING ligase modifies some substrates degradative chains, while other are modified non-degradative Here, using Arkadia related ligase, Ark2C, we characterize RING-E2 complexes promote assembly distinct linkages. Our structural studies highlight conserved interface, our binding activity assays reveal several E2 enzymes functionally interact Ark2C Arkadia. Using Arkadia, ubiquitylation differences ability be modified, Ubc13 Ube2K requiring addition 'priming' molecule before subsequent can occur. We also show bind tightly more extensively prior enhances modification. While further will understand pairing modulated cells, this study advances understanding recruitment chain provides tools may help dissect cellular function.

Язык: Английский

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Homozygous deleterious variants in MYCBPAP induce asthenoteratozoospermia involving abnormal acrosome biogenesis, manchette structure and sperm tail assembly in humans and mice

Science China Life Sciences, Год журнала: 2024, Номер unknown

Опубликована: Дек. 18, 2024

Язык: Английский

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