Structural and Functional Characteristics of TLR19 in Barbel Chub Compared to TLR19 in Grass Carp

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3103 - 3103

Опубликована: Март 27, 2025

The hybrid offspring of barbel chub Squaliobarbus curriculus and grass carp Ctenopharyngodon idella exhibit stronger resistance to the reovirus (GCRV) infection than carp. Toll-like receptors (TLRs) play indispensable roles in antiviral immunity fish. In this study, structures immune functions TLR19 (ScTLR19) (CiTLR19) were compared. amino acid sequence ScTLR19 shared high similarity (97.4%) identity (94.0%) with that CiTLR19, a phylogenetic tree revealed close evolutionary relationship between CiTLR19. Protein domain composition analyses showed possessed an additional leucine-rich repeat (designated as LRR9) located at positions 654-677 extracellular region, which was absent Multiple alignment three-dimensional structure comparison also indicated regions CiTLR19 exhibited greater differences compared their intracellular regions. Molecular docking region (docking score = -512.31) tendency for binding polyI:C, -474.90). Replacing LRR9 corresponding from reduced activity confirmed by ELISA. Moreover, overexpression experiments suggested could regulate both IRF3-TRIF IRF3-MyD88 signaling pathways during GCRV infection, while only regulated pathway. Importantly, replacing altered expression regulation on IRF3, MyD88, TRIF infection. These findings collectively reveal structural functional they may provide data support deeper understanding molecular mechanisms underlying carp, well genetic basis heterosis offspring.

Язык: Английский

Gut microbiota dysbiosis triggered by salinity stress enhances systemic inflammation in spotted scat (Scatophagus argus)

Fish & Shellfish Immunology, Год журнала: 2025, Номер unknown, С. 110353 - 110353

Опубликована: Апрель 1, 2025

Язык: Английский