Revitalising Aging Oocytes: Echinacoside Restores Mitochondrial Function and Cellular Homeostasis Through Targeting GJA1/SIRT1 Pathway DOI Creative Commons
Liuqing Yang,

Xinle Lai,

Fangxuan Lin

и другие.

Cell Proliferation, Год журнала: 2025, Номер unknown

Опубликована: Апрель 18, 2025

ABSTRACT As maternal age increases, the decline in oocyte quality emerges as a critical factor contributing to reduced reproductive capacity, highlighting urgent need for effective strategies combat aging. This study investigated protective effects and underlying mechanisms of Echinacoside (ECH) on aging oocytes. ECH significantly improved cytoskeletal stability chromosomal integrity, demonstrated by restored spindle morphology reinforced F‐actin structures, essential meiotic progression. It also preserved mitochondrial function restoring membrane potential dynamics, reducing ROS levels, downregulating DNA damage marker γ‐H2AX, thereby alleviating oxidative stress enhancing genomic stability. Furthermore, promoted cellular homeostasis through modulation lipid metabolism, autophagy lysosomal function. Transcriptomic analyses identified GJA1 pivotal mediator ECH's effects, validated molecular docking bio‐layer interferometry. Functional studies showed that inhibiting ability enhance first polar body extrusion rates, antioxidant validating role GJA1/SIRT1 pathway combating provides novel insights into rejuvenation highlights promising therapeutic candidate addressing age‐related challenges.

Язык: Английский

Plant Secondary Metabolites as Modulators of Mitochondrial Health: An Overview of Their Anti-Oxidant, Anti-Apoptotic, and Mitophagic Mechanisms DOI Open Access

Julia Anchimowicz,

Piotr Zielonka, Sławomir Jakieła

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(1), С. 380 - 380

Опубликована: Янв. 4, 2025

Plant secondary metabolites (PSMs) are a diverse group of bioactive compounds, including flavonoids, polyphenols, saponins, and terpenoids, which have been recognised for their critical role in modulating cellular functions. This review provides comprehensive analysis the effects PSMs on mitochondrial health, with particular emphasis therapeutic potential. Emerging evidence shows that these improve function by reducing oxidative stress, promoting biogenesis, regulating key processes such as apoptosis mitophagy. Mitochondrial dysfunction, hallmark many pathologies, neurodegenerative disorders, cardiovascular diseases, metabolic syndrome, has shown to benefit from protective PSMs. Recent studies show can dynamics, stabilise membranes, enhance bioenergetics, offering significant promise prevention treatment mitochondrial-related diseases. The molecular mechanisms underlying effects, modulation signalling pathways direct interactions proteins, discussed. integration into strategies is highlighted promising avenue improving efficacy while minimising side commonly associated synthetic drugs. also highlights need future research elucidate specific roles individual synergistic within complex plant matrices, may further optimise utility. Overall, this work valuable insights health potential natural agents targeting dysfunction.

Язык: Английский

Процитировано

3

Revitalising Aging Oocytes: Echinacoside Restores Mitochondrial Function and Cellular Homeostasis Through Targeting GJA1/SIRT1 Pathway DOI Creative Commons
Liuqing Yang,

Xinle Lai,

Fangxuan Lin

и другие.

Cell Proliferation, Год журнала: 2025, Номер unknown

Опубликована: Апрель 18, 2025

ABSTRACT As maternal age increases, the decline in oocyte quality emerges as a critical factor contributing to reduced reproductive capacity, highlighting urgent need for effective strategies combat aging. This study investigated protective effects and underlying mechanisms of Echinacoside (ECH) on aging oocytes. ECH significantly improved cytoskeletal stability chromosomal integrity, demonstrated by restored spindle morphology reinforced F‐actin structures, essential meiotic progression. It also preserved mitochondrial function restoring membrane potential dynamics, reducing ROS levels, downregulating DNA damage marker γ‐H2AX, thereby alleviating oxidative stress enhancing genomic stability. Furthermore, promoted cellular homeostasis through modulation lipid metabolism, autophagy lysosomal function. Transcriptomic analyses identified GJA1 pivotal mediator ECH's effects, validated molecular docking bio‐layer interferometry. Functional studies showed that inhibiting ability enhance first polar body extrusion rates, antioxidant validating role GJA1/SIRT1 pathway combating provides novel insights into rejuvenation highlights promising therapeutic candidate addressing age‐related challenges.

Язык: Английский

Процитировано

0