Current Proteomics,
Год журнала:
2023,
Номер
20(3), С. 169 - 183
Опубликована: Июнь 1, 2023
Introduction:
Excessive
oxidative
stress
is
always
associated
with
hepatic
disease,
including
hepatitis,
liver
fibrosis,
and
hepatocellular
carcinoma
(HCC).
Despite
this,
the
intricate
molecular
processes
driving
hepatocyte
apoptosis
due
to
remain
incompletely
comprehended.
Aim:
Consequently,
we
aimed
explore
role
of
miR-135a-5p
in
hepatoma
cells
(HepG2/3B).
Methods:
The
assessment
protein
expression
was
conducted
through
western
blotting.
Furthermore,
evaluated
RT-qPCR,
detection
performed
using
a
flow
cytometry
assay.
Result:
findings
suggest
connection
between
mitochondrial-driven
caspase
signaling
pathways.
suppression
inhibited
apoptotic
response
triggered
by
H2O2,
reactive
oxygen
species
(ROS)
generation,
as
well
decrease
mitochondrial
membrane
potential.
Conclusion:
Additionally,
knockdown
promoted
mitophagy
regulating
FoxO1/PINK1/Parkin
via
targeting
FoxO1.
To
conclude,
our
study
implied
that
miR-
135a-5p
might
function
probable
regulator
protects
against
stress.
Redox Biology,
Год журнала:
2024,
Номер
70, С. 103081 - 103081
Опубликована: Фев. 9, 2024
Heart
failure
with
preserved
ejection
fraction
(HFpEF)
is
a
devastating
health
issue
although
limited
knowledge
available
for
its
pathogenesis
and
therapeutics.
Given
the
perceived
involvement
of
mitochondrial
dysfunction
in
HFpEF,
this
study
was
designed
to
examine
role
dynamics
etiology
HFpEF.
Heart Failure Reviews,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 23, 2025
Abstract
With
rising
incidence,
mortality
and
limited
therapeutic
options,
heart
failure
with
preserved
ejection
fraction
(HFpEF)
remains
one
of
the
most
important
topics
in
cardiovascular
medicine
today.
Characterised
by
left
ventricular
diastolic
dysfunction
partially
due
to
impaired
Ca
2+
homeostasis,
ion
channel
particular,
SarcoEndoplasmic
Reticulum
-ATPase
(SERCA2a),
may
play
a
significant
role
its
pathophysiology.
A
better
understanding
complex
mechanisms
interplaying
contribute
SERCA2a
will
help
develop
treatments
targeting
it
thus
address
growing
clinical
challenge
HFpEF
poses.
This
review
examines
conflicting
evidence
present
for
changes
expression
activity
HFpEF,
explores
potential
underlying
mechanisms,
finally
evaluates
drug
gene
therapy
trials
failure.
Recent
positive
results
from
involving
widely
used
anti-diabetic
agents
such
as
sodium-glucose
co-transporter
protein
2
inhibitors
(SGLT2i)
glucagon-like
peptide-1
(GLP-1)
agonists
offer
advancement
management.
The
interplay
between
these
regulation
presents
novel
angle
that
could
open
new
avenues
modulating
function;
however,
mechanistic
research
this
emerging
field
is
limited.
Overall,
direct
undetermined,
highlighting
need
well-designed
pre-clinical
studies
robust
trials.
Biomolecules,
Год журнала:
2024,
Номер
14(4), С. 440 - 440
Опубликована: Апрель 4, 2024
Mitochondria,
the
energy
hubs
of
cell,
are
progressively
becoming
attractive
targets
in
search
for
potent
therapeutics
against
neurodegenerative
diseases.
The
pivotal
role
mitochondrial
dysfunction
pathogenesis
various
diseases,
including
Parkinson’s
disease
(PD),
underscores
urgency
discovering
novel
therapeutic
strategies.
Given
limitations
associated
with
available
treatments
dysfunction-associated
new
alternatives
has
become
imperative.
In
this
report,
we
embarked
on
an
extensive
screening
4224
fractions
from
384
Australian
marine
organisms
and
plant
samples
to
identify
natural
products
protective
effects
mitochondria.
Our
initial
using
PD
patient-sourced
olfactory
neurosphere-derived
(hONS)
cells
rotenone
as
a
mitochondria
stressor
resulted
108
promising
11
different
biota.
To
further
assess
potency
efficacy
these
hits,
biotas
were
subjected
subsequent
round
human
neuroblastoma
(SH-SY5Y)
cells,
6-hydroxydopamine
induce
stress,
complemented
by
membrane
potential
assay.
This
rigorous
process
yielded
35
active
eight
biotas.
Advanced
analysis
orbit
trap
mass
spectrophotometer
facilitated
identification
molecular
constituents
most
fraction
each
meticulous
approach
led
discovery
57
unique
compounds,
among
which
12
previously
recognized
their
mitoprotective
effects.
findings
highlight
vast
derived
plants
quest
innovative
targeting
Biomedicines,
Год журнала:
2024,
Номер
12(4), С. 806 - 806
Опубликована: Апрель 4, 2024
Heart
failure
(HF)
has
become
a
subject
of
continuous
interest
since
it
was
declared
new
pandemic
in
1997
because
the
exponential
increase
hospitalizations
for
HF
latest
years.
is
final
state
to
which
all
heart
diseases
different
etiologies
lead
if
not
adequately
treated.
It
highly
prevalent
worldwide,
with
progressive
age,
reaching
prevalence
10%
subjects
over
age
65
During
last
two
decades,
possible
see
that
preserved
ejection
fraction
(HFpEF)
increasing
while
reduced
(HFrEF)
decreasing.
HFpEF
typically
characterized
by
concentric
remodeling
left
ventricle
(LV)
impaired
diastolic
function
and
increased
filling
pressures.
Over
years,
also
insulin
resistance
(IR)/hyperinsulinemia
(Hyperins)
general
adult
population
progressively
increased,
primarily
due
lifestyle
changes,
particularly
developed
developing
countries,
range
globally
ranges
between
15.5%
46.5%.
Notably,
50%
patients
have
IR/Hyperins,
percentage
even
higher
those
HFpEF.
In
scientific
literature,
been
well
highlighted
circulating
levels
insulin,
associated
conditions
resistance,
are
responsible
cardiovascular
alterations
years
could
stimulate
development
and/or
worsening
The
aim
this
manuscript
review
literature
supports
pathophysiologic
connection
IR/Hyperins
community
toward
identification
hyperinsulinemia
as
an
independent
risk
factor
HF,
believing
its
adequate
screening
appropriate
treatment
reduce
improve
progression.
