Biomolecules,
Год журнала:
2024,
Номер
14(12), С. 1535 - 1535
Опубликована: Ноя. 30, 2024
Despite
significant
advances
in
drug
discovery
and
the
promising
antitumor
potential
of
combretastatin
A4
(CA-4),
which
selectively
targets
rapidly
dividing
cancer
cells,
CA-4’s
effects
on
non-dividing
human
such
as
peripheral
blood
mononuclear
cells
(PBMCs),
remain
unclear.
The
aim
this
study
is
to
evaluate
vitro
bioactivity
CA-4
PBMCs,
focusing
its
antigenotoxic
antioxidant
properties,
while
comparing
cytotoxic
potency
against
cell
lines
(JAR
HeLa),
normal
trophoblast
line
HTR-8/SVneo.
Cell
viability
metabolic
activity
were
evaluated
using
MTT
assay.
ROS
production
PBMCs
was
measured
H2DCFDA
assay,
DNA
damage
assessed
Comet
showed
cytotoxicity
HTR-8/SVneo
at
concentrations
above
200
µM,
JAR
HeLa,
100
µM
1
respectively.
also
reduced
levels
under
oxidative
stress
from
µM.
In
addition,
H2O2-induced
up
exhibited
lower
compared
inhibited
production,
providing
insight
into
therapeutic
efficacy
safety.
Biomolecules,
Год журнала:
2024,
Номер
14(12), С. 1535 - 1535
Опубликована: Ноя. 30, 2024
Despite
significant
advances
in
drug
discovery
and
the
promising
antitumor
potential
of
combretastatin
A4
(CA-4),
which
selectively
targets
rapidly
dividing
cancer
cells,
CA-4’s
effects
on
non-dividing
human
such
as
peripheral
blood
mononuclear
cells
(PBMCs),
remain
unclear.
The
aim
this
study
is
to
evaluate
vitro
bioactivity
CA-4
PBMCs,
focusing
its
antigenotoxic
antioxidant
properties,
while
comparing
cytotoxic
potency
against
cell
lines
(JAR
HeLa),
normal
trophoblast
line
HTR-8/SVneo.
Cell
viability
metabolic
activity
were
evaluated
using
MTT
assay.
ROS
production
PBMCs
was
measured
H2DCFDA
assay,
DNA
damage
assessed
Comet
showed
cytotoxicity
HTR-8/SVneo
at
concentrations
above
200
µM,
JAR
HeLa,
100
µM
1
respectively.
also
reduced
levels
under
oxidative
stress
from
µM.
In
addition,
H2O2-induced
up
exhibited
lower
compared
inhibited
production,
providing
insight
into
therapeutic
efficacy
safety.
