Extracellular Vesicles from Plasma of Patients with Glioblastoma Promote Invasion of Glioblastoma Cells Even After Tumor Resection DOI Creative Commons
Ekaterina N. Lyukmanova, Artem V. Kirichenko, Igor Medyanik

и другие.

Biomedicines, Год журнала: 2024, Номер 12(12), С. 2834 - 2834

Опубликована: Дек. 13, 2024

Background: Glioblastoma (GB) is a highly aggressive tumor, whose progression mediated by secretion of extracellular vesicles (EVs), which can pass the brain–blood barrier and be found in plasma. Here, we performed comparative analysis effects EVs from plasma healthy donors (hEVs) GB patients before (bEVs) after (aEVs) tumor surgical resection on invasion normal astrocytes cells. Methods: We transwell assay, analyzed MAP kinases activation Western blotting, studied SNAI1/SNAI2 cellular localization confocal microscopy, measured cadherins expression flow cytometry, cytokines, regulate migration inflammation, immunoassay. Results: hEVs did not affect cells, there was down-regulated astrocytes, while increased E- N-cadherin inflammation adhesion regulators both bEVs enhanced cells but via AKT, JNK1/2/3, p38 activation, stimulated clasterization SNAI1 cell nucleus, promoted an E/N cadherin switch, caused aEVs exhibited most pro-oncogenic (stimulation invasion, nuclear localization, JNK1/2/3 cells). However, were less pronounced than those bEVs. Conclusions: In our study, revealed common different plasma-derived hEVs, aEVs, stimulate some Being tumorigenic then bEVs, are still able to promote probably remaining resection.

Язык: Английский

Puzzle of Proteoform Variety—Where Is a Key? DOI Creative Commons
Stanislav Naryzhny

Proteomes, Год журнала: 2024, Номер 12(2), С. 15 - 15

Опубликована: Май 10, 2024

One of the human proteome puzzles is an imbalance between theoretically calculated and experimentally measured amounts proteoforms. Considering possibility combinations different post-translational modifications (PTMs), quantity possible proteoforms huge. An estimation gives more than a million in each cell type. But, it seems that there strict control over production maintenance PTMs. Although potential complexity due to PTMs tremendous, available information indicates only small part being implemented. As result, protein could have many according number modification sites, but because systems personal regulation, profile for given organism slightly different.

Язык: Английский

Процитировано

1

Recent Advancements and Innovations in Pediatric Precision Oncology DOI
Ross Mangum, Frank Y. Lin, D. Williams Parsons

и другие.

Journal of Pediatric Hematology/Oncology, Год журнала: 2024, Номер unknown

Опубликована: Июнь 10, 2024

Precision oncology incorporates comprehensive genomic profiling into the individualized clinical care of pediatric cancer patients. In recent years, pan-cancer analyses have led to successful implementation genomics-based trials and accelerated approval novel targeted agents. addition, disease-specific studies resulted in molecular subclassification myriad types with subsequent tailoring treatment intensity based on patient's prognostic factors. This review discusses progress field highlights developments that are leading more personalized improved patient outcomes. Increased understanding evolution precision over decades emphasizes tremendous impact applications. New technologies diagnostic modalities offer further promise for future advancements within field.

Язык: Английский

Процитировано

0

Advances in Fourier infrared spectroscopy for noninvasive diagnosis of diabetes mellitus: Analysis and prospects for application based on blood, urine and saliva samples DOI
Jing Yin, Guangwei Wang, Xianwen Zhang

и другие.

Microchemical Journal, Год журнала: 2024, Номер 207, С. 111764 - 111764

Опубликована: Сен. 24, 2024

Язык: Английский

Процитировано

0

Extracellular Vesicles from Plasma of Patients with Glioblastoma Promote Invasion of Glioblastoma Cells Even After Tumor Resection DOI Creative Commons
Ekaterina N. Lyukmanova, Artem V. Kirichenko, Igor Medyanik

и другие.

Biomedicines, Год журнала: 2024, Номер 12(12), С. 2834 - 2834

Опубликована: Дек. 13, 2024

Background: Glioblastoma (GB) is a highly aggressive tumor, whose progression mediated by secretion of extracellular vesicles (EVs), which can pass the brain–blood barrier and be found in plasma. Here, we performed comparative analysis effects EVs from plasma healthy donors (hEVs) GB patients before (bEVs) after (aEVs) tumor surgical resection on invasion normal astrocytes cells. Methods: We transwell assay, analyzed MAP kinases activation Western blotting, studied SNAI1/SNAI2 cellular localization confocal microscopy, measured cadherins expression flow cytometry, cytokines, regulate migration inflammation, immunoassay. Results: hEVs did not affect cells, there was down-regulated astrocytes, while increased E- N-cadherin inflammation adhesion regulators both bEVs enhanced cells but via AKT, JNK1/2/3, p38 activation, stimulated clasterization SNAI1 cell nucleus, promoted an E/N cadherin switch, caused aEVs exhibited most pro-oncogenic (stimulation invasion, nuclear localization, JNK1/2/3 cells). However, were less pronounced than those bEVs. Conclusions: In our study, revealed common different plasma-derived hEVs, aEVs, stimulate some Being tumorigenic then bEVs, are still able to promote probably remaining resection.

Язык: Английский

Процитировано

0