In Vitro Culture of Human Dermal Fibroblasts on Novel Electrospun Polylactic Acid Fiber Scaffolds Loaded with Encapsulated Polyepicatechin Physical Gels DOI Creative Commons
Eliza Miranda-Buendía, Gertrudis Hortensia González-Gómez, Alfredo Maciel-Cerda

и другие.

Gels, Год журнала: 2024, Номер 10(9), С. 601 - 601

Опубликована: Сен. 20, 2024

Polyepicatechin (PEC) in a hydrogel has previously shown promise enhancing physiological properties and scaffold preparation. However, it remains unclear whether PEC-based fibers can be applied skin tissue engineering (STE). This study aimed to synthesize characterize electrospun PEC physical gels polylactic acid (PLA) scaffolds (PLAloadedPECsub) for potential use as constructs with human dermal fibroblasts (HDFs). was produced through enzymatic polymerization, confirmed by Fourier transform infrared (FTIR) spectroscopy. Scanning electron microscopy (SEM) demonstrated the feasibility of producing PLAloadedPECsub electrospinning. The metabolic activity viability HDFs cocultured indicate that is promising STE.

Язык: Английский

Microencapsulation of Spent Coffee Extract Within Saccharomyces cerevisiae Cells via Spray Drying and Evaluation of Its In Vitro Bioaccessibility DOI Creative Commons

Isabel H. Chacón-Figueroa,

Ramón Francisco Dórame‐Miranda, Guadalupe Amanda López‐Ahumada

и другие.

Foods, Год журнала: 2025, Номер 14(6), С. 1053 - 1053

Опубликована: Март 19, 2025

Spent coffee is rich in bioactive compounds, including chlorogenic acid, caffeic and caffeine, which offer health benefits. However, digestive processes can degrade these compounds; therefore, microencapsulation within Saccharomyces cerevisiae yeast cells offers a novel method to stabilize compounds during digestion. In fact, it important mention that this technique of has not been previously applied spent extract. As result, study, extract was microencapsulated non-plasmolyzed (NPCs) plasmolyzed (PCs) using the spray drying method. The physicochemical properties microencapsulates were characterized, bioaccessibility evaluated with digestion vitro. Encapsulation efficiency (EE) 38.62% for NPCs 55.78% PCs, loading capacities (LCs) 126.36 242 g/kg, respectively (according Equations (1) (2)). presence antioxidant identified by HPLC coffee, confirmed FTIR. vitro assays revealed higher intestinal phase, greater than 90%, increased activity beer made microcapsules (BPM). These results suggest effectively stabilizes extract, releasing them throughout gastrointestinal tract vitro, mainly phase. Thus, could serve as functional additives good percentage bioaccessibility.

Язык: Английский

Процитировано

0

Recent Application of Electrospray Technology in Food Processing: A Review DOI
Aiping Liu, Bo Wang, Yuchuan Wang

и другие.

Food and Bioprocess Technology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 16, 2025

Язык: Английский

Процитировано

0

Encapsulation of hydrophobic compounds in yeast cells: methods, characterization, and applications DOI Creative Commons
Deniz Günal‐Köroğlu, Ayşenur Betül Bilgin, Gülşah Karabulut

и другие.

Опубликована: Июнь 20, 2024

Encapsulation is a pivotal technique for protecting and enhancing the efficiency of sensitive natural bioactive substances, notably essential oils, vitamins, phenolic compounds, widely used in foods nutraceuticals. Critical considerations selecting encapsulation agents encompass safety, release kinetics, stability, cost-effectiveness. Yeast cells emerge as versatile carriers distinguished by their low cost, compatibility with biological systems, eco-friendly degradation properties, accommodating both hydrophilic hydrophobic agents. Various yeast strains, including Saccharomyces cerevisiae, Torulopsis lipofera, Cutaneotrichosporon curvatus, Yarrowia lipolytica, Candida utilis, find utility microencapsulation. cell relies on permeation through walls, predominantly composed mannoproteins polysaccharides. The process includes passive or vacuum-infused diffusion compounds inside cells, precise droplet size control, attractive forces to trap components within cellular structures. display versatility various states, whether alive dead, intact plasmolyzed. In addition, loading capacity bioactives can be increased chemical pretreatment techniques such plasmolysis, autolysis, enzyme hydrolysis, freeing up space eliminating water-soluble components. summary, presents promising sustainable technology diverse applications food industry. enhance stability controlled magnifying efficacy like curcumin, β-carotene, vitamin D across products. This comprehensive review focuses procedures, influential factors, characterization techniques, applications, pronounced emphasis materials.

Язык: Английский

Процитировано

3

In Vitro Culture of Human Dermal Fibroblasts on Novel Electrospun Polylactic Acid Fiber Scaffolds Loaded with Encapsulated Polyepicatechin Physical Gels DOI Creative Commons
Eliza Miranda-Buendía, Gertrudis Hortensia González-Gómez, Alfredo Maciel-Cerda

и другие.

Gels, Год журнала: 2024, Номер 10(9), С. 601 - 601

Опубликована: Сен. 20, 2024

Polyepicatechin (PEC) in a hydrogel has previously shown promise enhancing physiological properties and scaffold preparation. However, it remains unclear whether PEC-based fibers can be applied skin tissue engineering (STE). This study aimed to synthesize characterize electrospun PEC physical gels polylactic acid (PLA) scaffolds (PLAloadedPECsub) for potential use as constructs with human dermal fibroblasts (HDFs). was produced through enzymatic polymerization, confirmed by Fourier transform infrared (FTIR) spectroscopy. Scanning electron microscopy (SEM) demonstrated the feasibility of producing PLAloadedPECsub electrospinning. The metabolic activity viability HDFs cocultured indicate that is promising STE.

Язык: Английский

Процитировано

0