Network pharmacology combined with experimental verification for exploring the potential mechanism of phellodendrine against depression

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 14, 2025

The anti-inflammatory effect of phellodendrine (PHE), derived from Phellodendri Chinensis Cortex, has been verified in previous studies. Major depressive disorder (MDD) is associated with immune dysregulation and inflammatory processes. This study aimed to explore the therapeutic effects PHE on MDD through network pharmacology experimental validation. Multiple databases were used predict targets MDD. intersection between obtained identify as for against MDD, followed by protein–protein interaction network, Gene Ontology Kyoto Encyclopedia Genes Genomes pathway analyses. Molecular docking was applied further confirm anti-MDD PHE. mitochondrial DNA (mtDNA) copy number, cytokines pathway-related mRNA expressions PC12 cell determined via quantitative PCR (qPCR) enzyme-linked immunosorbent assay verify our finding. Thirty-eight exerted an regulating SLC6A4, SLC6A3, SLC6A2, MAOA other serotonergic synapse, salivary secretion, dopaminergic cAMP signalling pathway. In vitro, induced increment mtDNA number compared CORT group. affected levels IL6 IL1β different concentrations. CHRM1, HTR1A key PI3K/Akt also influenced. Our research reveals novel mechanisms underlying experiments, which provides a new direction development antidepressants.

Язык: Английский

Phellodendrine Exerts Protective Effects on Intra-abdominal Sepsis by Inactivating AKT/NF-kB Signaling

Cell Biochemistry and Biophysics, Год журнала: 2025, Номер unknown

Опубликована: Фев. 14, 2025

Язык: Английский