Stem Cell-Based Therapy and Cell-Free Therapy as an Alternative Approach for Cardiac Regeneration

Stem Cells International, Год журнала: 2023, Номер 2023, С. 1 - 17

Опубликована: Ноя. 2, 2023

The World Health Organization reports that cardiovascular diseases (CVDs) represent 32% of all global deaths. ineffectiveness conventional therapies in CVDs encourages the development novel, minimally invasive therapeutic strategies for healing and regeneration damaged tissue. self-renewal capacity, multilineage differentiation, lack immunogenicity, immunosuppressive properties mesenchymal stem cells (MSCs) make them a promising option CVDs. However, growing evidence suggests myocardial occurs through paracrine factors extracellular vesicle (EV) secretion, rather than differentiation into cardiomyocytes. Research shows secrete or surface-shed their culture media various cytokines, chemokines, growth factors, anti-inflammatory EVs, which constitute an MSC-conditioned medium (MSC-CM) secretome. use MSC-CM enhances cardiac repair resident heart cell proliferation, scar mass reduction, decrease infarct wall thickness, function improvement comparable to MSCs without side effects. This review highlights limitations benefits based on secretome as innovative treatment

Язык: Английский

---

“Time Is out of Joint” in Pluripotent Stem Cells: How and Why

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(4), С. 2063 - 2063

Опубликована: Фев. 8, 2024

The circadian rhythm is necessary for the homeostasis and health of living organisms. Molecular clocks interconnected by transcription/translation feedback loops exist in most cells body. A puzzling exemption to this, otherwise, general biological hallmark given cell physiology pluripotent stem (PSCs) that lack oscillations gradually acquired following their vivo programmed differentiation. This process can be nicely phenocopied vitro commitment reversed during reprogramming somatic induce PSCs. current understanding how why pluripotency “time-uncoupled” largely incomplete. complex picture emerging where core clockwork negatively regulated PSCs at post-transcriptional/translational, epigenetic, other-clock-interaction levels. Moreover, non-canonical functions core-work components balance between identity metabolic-driven are emerging. review selects discusses results relevant recent investigations providing major insights into this context.

Язык: Английский

---

Platycodi radix aqueous extract salvages doxorubicin‐induced senescence by mitochondrial reactive oxygen species reduction in umbilical cord matrix stem cells

Environmental Toxicology, Год журнала: 2024, Номер 39(7), С. 3872 - 3882

Опубликована: Апрель 1, 2024

Abstract Platycodi radix is a widely used herbal medicine that contains numerous phytochemicals beneficial to health. The health and biological benefits of P. have been found across various diseases. utilization umbilical cord stromal stem cells, derived from Wharton's jelly the human cord, has emerged as promising approach for treating degenerative Nevertheless, growing evidence indicates function cells declines with age, thereby limiting their regenerative capacity. primary objective in this study investigate effects senescent cells. We conducted experiments showcase diminished levels Lamin B1 Sox‐2, along an elevation p21, which serve indicative markers Our findings revealed loss coupled increase subjected low‐dose (0.1 μM) doxorubicin (Dox) stimulation. However, restored Dox‐damage reversed conditions when exposed Dox‐induced reactive oxygen species (ROS) mitochondrial membrane potential are significantly changed. In Dox‐challenged aged reduced senescence, increased longevity, prevented dysfunction ROS protected against senescence‐associated apoptosis. This suggests might be therapeutic rescue agent aging effect Inhibition cell death, aging‐associated provides additional insights into underlying molecular mechanisms.

Язык: Английский

---

Role of umbilical cord mesenchymal stromal cells in skin rejuvenation

npj Regenerative Medicine, Год журнала: 2024, Номер 9(1)

Опубликована: Май 10, 2024

Abstract Aging is the main cause of many degenerative diseases. The skin largest and most intuitive organ that reflects aging body. Under interaction endogenous exogenous factors, there are cumulative changes in structure, function, appearance skin, which characterized by decreased synthesis collagen elastin, increased wrinkles, relaxation, pigmentation, other characteristics. inevitable, but it can be delayed. successful isolation mesenchymal stromal cells (MSC) 1991 has greatly promoted progress cell therapy human International Society for Cellular Therapy (ISCT) points out MSC a kind pluripotent progenitor have self-renewal ability (limited) vitro potential differentiation. This review mainly introduces role perinatal umbilical cord-derived MSC(UC-MSC) field rejuvenation. An in-depth systematic understanding mechanism UC-MSCs against great significance early realization clinical transformation UC-MSCs. paper summarized characteristics rejuvenation reported recent years. In order to provide reference further research delay aging.

Язык: Английский

---

DNA Nanoparticle Based 2D Biointerface to Study the Effect of Dynamic RGD Presentation on Stem Cell Adhesion and Migration

Small, Год журнала: 2024, Номер unknown

Опубликована: Май 17, 2024

Abstract The native extracellular matrix (ECM) undergoes constant remodeling, where adhesive ligand presentation changes over time and in space to control stem cell function. As such, it is of interest develop 2D biointerfaces able study these complex stem‐cell interactions. In this study, a novel dynamic bio interface based on DNA hybridization developed, which can be employed display kinetics used cell‐ligand interaction. approach, mesoporous silica nanoparticles (MSN) are functionalized with single‐strand (MSN‐ssDNA) spin‐coated glass substrate create the interface. Cell tripeptide RGD conjugated complementary strands (csDNA) 9, 11, or 20 nucleotides length, form csDNA‐RGD. resulting 3 csDNA‐RGD conjugates hybridize ssDNA MSN surface, presenting increased dissociation rates as length shortened. Slow led enhanced adhesion spreading, elongated morphology. Cells surfaces slow also exhibited higher motility, migrating multiple directions compared cells fast rates. This contributes existing body knowledge ligand‐stem

Язык: Английский

---

Epidemiological exploration of the impact of bluetooth headset usage on thyroid nodules using Shapley additive explanations method

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Июнь 21, 2024

With an increasing prevalence of thyroid nodules globally, this study investigates the potential correlation between use Bluetooth headsets and incidence nodules, considering cumulative effects non-ionizing radiation (NIR) emitted by these devices. In study, we analyzed 600 valid questionnaires from WenJuanXing platform using Propensity Score Matching (PSM) XGBOOST model, supplemented SHAP analysis, to assess risk nodules. PSM was utilized balance baseline characteristic differences, thereby reducing bias. The model then employed predict factors, with efficacy measured area under Receiver Operating Characteristic (ROC) curve (AUC). analysis helped quantify explain impact each feature on prediction outcomes, identifying key factors. Initially, underwent processing, resulting in a matched dataset 96 cases for modeling analysis. AUC value reached 0.95, demonstrating high accuracy differentiating nodule risks. revealed age daily headset usage duration as two most significant factors affecting risk. Specifically, longer durations were strongly linked increased developing indicated outcomes. Our highlighted relationship prolonged risk, emphasizing importance health impacts modern technology, especially devices like that are frequently used daily. Through precise predictions variable our research provides scientific basis formulation public policies personal habit choices, suggesting attention should be paid life reduce Future further investigate biological mechanisms consider additional influencing offer more comprehensive guidance preventive measures.

Язык: Английский

---

Extracellular Vesicles Enhance Osteogenic Differentiation of Mesenchymal Stem Cells without any Chemical Agents

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Янв. 2, 2025

The use of extracellular vesicles (EVs) in bone tissue engineering is emerging as a promising alternative strategy to stem cells. For clinical application, EVs must be biomanufactured from suitable source cells, systematically characterized, and validated for their efficacy regeneration. This study focuses on the possibility this translation using vitro methods with rat marrow cells (rBMSCs). Bone was harvested Wistar rats cultured direct adherence method. rBMSCs were characterized through trilineage differentiation, flow cytometry, immunofluorescence, real-time PCR. optimal isolation method derived rBMSC investigated. isolated ultracentrifugation yielded homogeneous good quality quantity. Nanoparticle Tracking Analysis, Dynamic Light Scattering, Transmission Electron Microscopy, Western Blot analysis. osteogenic differentiation evaluated EVs, confirmed by MTT Cell Proliferation Assay In Osteogenesis Assays. concentration- time-dependent enhancement rBMSC-derived also examined. Most importantly, promoted without adding any conventional chemical agents culture media. These findings will pave way further investigations link EVs' therapeutic benefits related applications.

Язык: Английский

---

Harnessing the ubiquitin proteasome system as a key player in stem cell biology

BioFactors, Год журнала: 2025, Номер 51(1)

Опубликована: Янв. 1, 2025

Abstract Intracellular proteins take part in almost every body function; thus, protein homeostasis is of utmost importance. The ubiquitin proteasome system (UPS) has a fundamental role homeostasis. Its main to selectively eradicate impaired or misfolded proteins, thus halting any damage that could arise from the accumulation these malfunctioning proteins. Proteasomes have critical controlling all cell types, including stem cells. We will discuss UPS enzymes as well 26S complex biology several angles. First, we shall overview common trends proteasomal activity and gene expression different subunits upon passaging differentiation cells toward various lineages. Second, explore effect modulating navigate through interrelation between proteasomes' proteasome‐related transcription factors. Third, shed light on curated microRNAs long non‐coding RNAs using bioinformatics tools might possible regulating possibly, manipulation, can enhance process into lineages and/or delay senescence passaging. This help decipher played by individual interrelated molecular mediators cells' maintenance open new avenues research. ultimately provide leap developing novel therapeutic interventions related dysregulation.

Язык: Английский

---

Anti‐skin aging effects of Gosori liquor lees extract by regulating interactions between senescent fibroblasts and adipose‐derived stem cells

International Journal of Cosmetic Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 21, 2025

Abstract When cellular ageing is accelerated by various extrinsic/endogenous stimuli, regenerative function deteriorates, and enriched secretomes, such as the senescence‐associated secretory phenotype (SASP), contribute to chronic inflammation cause matrix degeneration. SASPs from senescent fibroblasts exacerbate senescence via autocrine signalling also accelerate skin through induction of neighbouring cell paracrine signalling. The interaction between dermis their neighbours, adipose‐derived stem cells (ADSCs) in hypodermis, which lies deep dermis, a potential target for ageing. In this study, we observed that an extract lees Gosori liquor (GLE), traditional Korean made fermenting millet rice, suppressed fibroblasts, including SASP production, replicative model. We further examined whether anti‐ageing effects GLE on affected surrounding cells, ADSCs. results showed factors ADSCs were culture medium (SF‐CM) treated with compared SF‐CM‐only group. Furthermore, ability was promoted GLE‐treated SF‐CM ADSC migration stimulated upregulating levels α‐smooth muscle actin, collagen type I alpha 2, vascular endothelial growth factor expression PI3K/AKT pathway. Those indicate can exert regulating adipocyte interactions, improving senescence. conducted clinical trial subjects over 45 years age confirm vivo changes parameters, wrinkles volume face (IRB No. DM‐IRB‐2023‐809‐01‐T1). GLE‐containing cream more effective wrinkles, elasticity, density, thickness, around sunken eyes after 4 weeks use than placebo. conclusion, plays important role inhibiting transition be promising strategy.

Язык: Английский

---

Update of Aging Hallmarks in Idiopathic Pulmonary Fibrosis

Cells, Год журнала: 2025, Номер 14(3), С. 222 - 222

Опубликована: Фев. 5, 2025

Idiopathic Pulmonary Fibrosis (IPF) is an epithelial-driven interstitial lung disease of unknown etiology characterized by the excessive proliferation fibroblast populations that synthesize large amounts extracellular matrix. In this devastating disorder, all aging hallmarks appear prematurely or are altered. This review highlights key findings about IPF characteristics recently recognized as aging, including mechanical alterations, inflammaging, dysbiosis, alternative splicing, and disabled macroautophagy. It also revisits classic which encompass stem cell exhaustion, cellular senescence, altered intercellular communication. Enhancing our understanding fundamental processes underlie in may facilitate development innovative experimental strategies to improve therapeutic outcomes.

Язык: Английский

---