Real-Time Wireless Sensing of Cardiomyocyte Contractility by Integrating Magnetic Microbeam and Oriented Nanofibers

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(35), С. 45861 - 45870

Опубликована: Авг. 23, 2024

In vitro cardiomyocyte mechano-sensing platform is crucial for evaluating the mechanical performance of cardiac tissues and will be an indispensable tool application in drug discovery disease mechanism study. Magnetic sensing offers significant advantages real-time, situ wireless monitoring resistance to ion interference. However, due mismatch between stiffness traditional rigid magnetic material myocardial tissue, sensitivity insufficient it difficult achieve cell structure induction three-dimensional cultivation. Herein, a that integrates neodymium–iron–boron/polydimethylsiloxane (NdFeB/PDMS) flexible microbeam with suspended ordered polycaprolactone (PCL) nanofiber membranes was developed, providing anisotropic culture environment growth simultaneously realizing contractility monitoring. The as-prepared sensor presented ultrahigh 442.2 μV/μm deflection resolution 2 μm. By continuously status stimulation feedback, we verified capability capture dynamic changes contractility. This provides perspective maturity performance.

Язык: Английский

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Primary Bone Tumors and Breast Cancer-Induced Bone Metastases: In Vivo Animal Models and New Alternative Approaches

Biomedicines, Год журнала: 2024, Номер 12(11), С. 2451 - 2451

Опубликована: Окт. 25, 2024

Bone is the preferential site of metastasis for most common tumors, including breast cancer. On other hand, osteosarcoma primary bone cancer that commonly occurs and causes cancer-related deaths in children. Several treatment strategies have been developed so far, with little or no efficacy patient survival development side effects. Therefore, there an urgent need to develop more effective therapies tumors metastatic disease. This almost necessarily requires use vivo animal models better mimic human pathology at same time follow ethical principles humane testing. In this review we aim illustrate main suitable employed model metastases osteosarcoma. We will also take a look recent technologies implemented partial replacement

Язык: Английский

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Organoids and 3D In Vitro Models as a Platform for Precision Medicine (PM): An Update

Organoids, Год журнала: 2024, Номер 3(3), С. 165 - 173

Опубликована: Авг. 1, 2024

Globally, a number of diseases impact us and while treatment options exist, it is often found that similar treatments have variable effects on different patients with the same disease. Particularly in case conditions are closely associated genetics (like cancer), intensity results vary between patients. Even for like arthritis not uncommon only fraction to achieve remission therapeutic approach. With millions suffering from cancer arthritis, precision medicine (PM) has been at forefront biomedical pharmaceutical research since 2015. PM focusses understanding genetic environmental factors affecting several platforms. One platforms use three-dimensional (3D) vitro models, especially those derived patient themselves. These organ-on-chip (OOC), organoid spheroid 3D biomaterial scaffolds others, advantages over traditional two-dimensional (2D) cell culture approaches. In this opinion paper, author briefly discusses used PM. Then, models 2D vivo considered an overview their applications provided. Finally, outlines challenges future directions shares about using as tool towards enhanced outcomes.

Язык: Английский

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The Efficiency of In Vitro Differentiation of Primate iPSCs into Cardiomyocytes Depending on Their Cell Seeding Density and Cell Line Specificity

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(15), С. 8449 - 8449

Опубликована: Авг. 2, 2024

A thorough characterization of induced pluripotent stem cells (iPSCs) used with in vitro models or therapeutics is essential. Even iPSCs derived from a single donor can exhibit variability within and between cell lines, which lead to heterogeneity results hinder the promising future replacement therapies. In this study, seeding density human rhesus monkey was tested maximize line-specific yield generated cardiomyocytes. We found that, despite using same iPSC generation differentiation protocols, for best efficiency could differ by factor four lines here. addition, showed differences range densities that they tolerate without severe loss efficiency. Overall, our data show critical parameter inefficiency primate cardiomyocytes episomal approach still considerable heterogeneity. Therefore, individual required, functional comparability vivo processes must be ensured warrant translatability research iPSCs.

Язык: Английский

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Generation of induced pluripotent stem cells from the Asian bats

International Journal of Veterinary Science and Medicine, Год журнала: 2024, Номер 12(1), С. 81 - 90

Опубликована: Авг. 12, 2024

Preservation of native Korean bats is crucial for maintaining ecological balance, as they play a vital role in insect control, pollination, and seed dispersal within their ecosystems. The present study details the establishment bat induced pluripotent stem cells (BatiPSCs) from two Asian (

Язык: Английский

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Reprogramming of DPSC to Induced Pluripotent Stem Cells

Springer eBooks, Год журнала: 2024, Номер unknown, С. 157 - 183

Опубликована: Янв. 1, 2024

Язык: Английский

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