Transcriptome analysis of granulation tissue from periodontal osseous defects

Journal of Periodontology, Год журнала: 2025, Номер unknown

Опубликована: Май 12, 2025

Abstract Background Granulation tissue is routinely discarded in periodontal surgical procedures, but few studies have characterized it. The present study aimed to compare global gene expression granulation derived from different types of osseous defects. Methods Total RNA was isolated harvested during routine procedures. Nineteen sites were analyzed—seven infrabony, six suprabony, and furcation Following quality control checks, samples underwent bulk mRNA sequencing (20–30 million read pairs per sample) before bioinformatic analyses utilizing ontology associated pathway/enrichment analyses. Results No statistically significant differentially expressed genes (DEG) detected between defect types. An increase the 11 with a false discovery rate <0.05 when comparison made terms healing duration post nonsurgical therapy (NSPT). Notably, involved included those regulating collagen synthesis osteogenic activity. Analysis based on sex differences revealed 38 DEG. Gene enrichment analysis that 24 DEG without Y‐linked attachment are mostly immune regulation. Conclusions Routinely exhibits epithelial characteristics due substantial period maturation NSPT. Confirmation ongoing beyond traditional re‐evaluation timepoints warrants further investigation differentiation potential. More research needed elucidate role as biological variable affecting response. Plain language summary (wound) removed gum (periodontal) knowledge its scarce. This bone defects via high‐throughput sequencing. extracted 95 disease patients surgery. After 19 (seven defects) eligible for Subsequent done software known cell pathways processes. no category. There significantly increased following deep scaling treatment. These Interestingly, association Y chromosomes regulation system lining change over time unravel response this type tissue.

Язык: Английский

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A complex relationship among periodontal disease, obesity and diabetes

International Journal of Applied Dental Sciences, Год журнала: 2024, Номер 10(2), С. 402 - 406

Опубликована: Янв. 1, 2024

Introduction: The relationship between periodontal disease, obesity and diabetes is crucial because of their mutual negative impact. Periodontitis worsens glycemic control in diabetics increases the risk developing diabetes. Obesity, turn, susceptibility to disease.Objective: To analyze literature on association diabetes, mainly its diagnosis, prevention treatment.Methodology: A search articles published last 5 years was carried out using PubMed, Scopus Google Scholar databases. implemented Boolean operators AND, OR, NOT. Keywords used for included: "periodontal disease", "obesity", "diabetes", "diagnosis", "prevention", "treatment".Results: Early accurate diagnosis essential proper patient health management. Periodontal examination, HbA1c measurement waist circumference index assessment are tools identify individuals at these conditions take preventive measures or initiate timely treatment. Treatment disease people with requires a comprehensive approach that includes control, healthy diet, regular physical activity, oral hygiene dental visits. Implementing strategies can improve health, reduce systemic inflammation decrease complications associated chronic diseases.Conclusions: interconnected. key preventing managing diseases. Lifestyle changes, medical care essential. Improving reduces inflammation, complications.

Язык: Английский

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DETECTION OF STRUCTURAL AND FUNCTIONAL DISORDERS IN MITOCHONDRIA OF PERIODONTAL TISSUE IN AN EXPERIMENTAL MODEL OF PERIODONTITIS

Russian Journal of Dentistry, Год журнала: 2024, Номер unknown

Опубликована: Сен. 20, 2024

BACKGROUND: Recently, more and evidence has emerged indicating that mitochondrial dysfunction plays an important role in the development progression of inflammatory diseases oral cavity such as periodontitis, pulpitis. AIM: To evaluate structural functional disorders mitochondria periodontal tissues experimental model periodontitis laboratory rats. METHODS: Male white Wistar rats weighing 221 ± 7.5 g at age 4 months were used this work. The animals divided by simple randomization into two groups, 10 each: group 1 – intact (control group); Group 2 with simulated periodontitis. Experimental was modeled using ligature method sewing a polyfilament non-absorbable thread gum area mandibular incisors. adequacy reproduced animal assessed histological analyses. following molecular genetics biochemical parameters assessed: damage to nuclear DNA (nDNA) (mtDNA), copy number degree heteroplasmy mtDNA, expression genes, well levels hydrogen peroxide (H2O2), reduced glutathione (GSH) malondialdehyde (MDA). RESULTS: work shows resulting changes tissue identified, which indicated formation animals. results analyzes showed on 14th day after application ligature, increased level mtDNA recorded tissue, compared control group. In these same animals, decrease genes involved ATP synthesis observed. At time, GSH while H2O2 MDA CONCLUSIONS: Structural revealed Thus, new strategies for assessing function may help diagnosis treatment disease, its complications.

Язык: Английский

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Genetic Predisposition to Prediabetes in the Kazakh Population

Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(10), С. 10913 - 10922

Опубликована: Сен. 28, 2024

The aim of this study was to conduct a comparative analysis the population frequencies minor allele polymorphic variants in genes

Язык: Английский

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