Repair of spinal cord injury by bone marrow mesenchymal stem cell-derived exosomes: a systematic review and meta-analysis based on rat models DOI Creative Commons

Zhongduo Ye,

Yukun Zheng, Ningning Li

и другие.

Frontiers in Molecular Neuroscience, Год журнала: 2024, Номер 17

Опубликована: Авг. 7, 2024

Objective This study aims to systematically evaluate the efficacy of bone marrow mesenchymal stem cell-derived exosomes (BMSCs-Exo) in improving spinal cord injury (SCI) mitigate risk translational discrepancies from animal experiments clinical applications. Methods We conducted a comprehensive literature search up March 2024 using PubMed, Embase, Web Science, and Scopus databases. Two researchers independently screened literature, extracted data, assessed quality studies. Data analysis was performed STATA16 software. Results A total 30 studies were included. The results indicated that BMSCs-Exo significantly improved BBB score SCI rats (WMD = 3.47, 95% CI [3.31, 3.63]), inhibited expression pro-inflammatory cytokine TNF- α (SMD -3.12, [−3.57, −2.67]), promoted anti-inflammatory cytokines IL-10 2.76, [1.88, 3.63]) TGF- β 3.89, [3.02, 4.76]). Additionally, reduced apoptosis levels −4.52, [−5.14, −3.89]), axonal regeneration markers NeuN cells/field 3.54, [2.65, 4.42]), NF200 4.88, [3.70, 6.05]), number Nissl bodies 1.89, [1.13, 2.65]), decreased astrogliosis marker GFAP −5.15, [−6.47, −3.82]). heterogeneity among primarily due variations transplantation doses, with increasing higher doses. Conclusion motor function by modulating inflammatory responses, reducing apoptosis, inhibiting astrogliosis, promoting regeneration. However, presence selection, performance, detection biases current may undermine evidence this study.

Язык: Английский

Pre-treated Mesenchymal Stem Cell-Derived Exosomes: A New Perspective for Accelerating Spinal Cord Injury Repair DOI Creative Commons

Zhiqiang Liao,

Jinwei Zeng,

Aiqing Lin

и другие.

European Journal of Pharmacology, Год журнала: 2025, Номер 992, С. 177349 - 177349

Опубликована: Фев. 5, 2025

Spinal cord injury (SCI) is a devastating event for the central nervous system (CNS), often resulting in loss of sensory and motor functions. It profoundly affects both physiological psychological well-being patients, reducing their quality life while also imposing significant economic pressure on families healthcare system. Due to complex pathophysiology SCI, effective treatments promoting recovery remain scarce. Mesenchymal stem cell-derived exosomes (MSC-Exos) offer advantages such as low immunogenicity, good biocompatibility, ability cross blood-spinal barrier (BSCB). In preclinical studies, they have progressively shown efficacy SCI repair functional recovery. However, yield insufficient targeting MSC-Exos limit therapeutic efficacy. Currently, genetic engineering other preprocessing techniques are being employed optimize properties exosomes, thereby enhancing potential. Therefore, this paper provides an overview biogenesis exosomes. summarizes current approaches optimizing exosome performance. Additionally, it details mechanisms through which optimized provide neuroprotection explores potential combined involving hydrogels.

Язык: Английский

Процитировано

0
PTGS2 Silencing Inhibits Ferroptosis in Staphylococcus Aureus-induced Osteomyelitis By Blocking the IL-17A Signaling Pathway DOI Creative Commons
Sirui Zhou, Wenguang Li, Lidan Yang

и другие.

Inflammation, Год журнала: 2025, Номер unknown

Опубликована: Апрель 21, 2025

Osteomyelitis caused by Staphylococcus aureus (S. aureus) infection is an inflammatory bone disease characterized continuous destruction, which difficult to treat. This research aimed explore the molecular mechanisms of S. aureus-induced osteomyelitis. Using GSE166522 and GSE227521 datasets, hub differentially expressed genes (DEGs) were screened bioinformatics analysis. Hub gene expression levels validated in mouse models. An inhibitor PTGS2, etoricoxib, was used assess role PTGS2 osteomyelitis model. silenced LPS-induced MC3T3-E1 cell model study its effect on function. Six screened, including ARG1, TIMP1, NOS2, SOCS3, IL1B, highly Etoricoxib treatment attenuated infiltration tibial tissue mice with In vivo vitro, etoricoxib silencing reduced factor (TNF-α, IL-1β, IL-6) levels. promoted viability inhibited apoptosis ferroptosis. GPX4 SLC7A11 protein significantly increased after silencing. Mechanistically, IL-17A intervention counteracted impact behaviors secukinumab combined more effectively suppressed inflammation ferroptosis, indicating that impeded progression inhibiting pathway. Silencing reduces ferroptosis obstructing pathway, suggests a new approach for

Язык: Английский

Процитировано

0
Repair of spinal cord injury by bone marrow mesenchymal stem cell-derived exosomes: a systematic review and meta-analysis based on rat models DOI Creative Commons

Zhongduo Ye,

Yukun Zheng, Ningning Li

и другие.

Frontiers in Molecular Neuroscience, Год журнала: 2024, Номер 17

Опубликована: Авг. 7, 2024

Objective This study aims to systematically evaluate the efficacy of bone marrow mesenchymal stem cell-derived exosomes (BMSCs-Exo) in improving spinal cord injury (SCI) mitigate risk translational discrepancies from animal experiments clinical applications. Methods We conducted a comprehensive literature search up March 2024 using PubMed, Embase, Web Science, and Scopus databases. Two researchers independently screened literature, extracted data, assessed quality studies. Data analysis was performed STATA16 software. Results A total 30 studies were included. The results indicated that BMSCs-Exo significantly improved BBB score SCI rats (WMD = 3.47, 95% CI [3.31, 3.63]), inhibited expression pro-inflammatory cytokine TNF- α (SMD -3.12, [−3.57, −2.67]), promoted anti-inflammatory cytokines IL-10 2.76, [1.88, 3.63]) TGF- β 3.89, [3.02, 4.76]). Additionally, reduced apoptosis levels −4.52, [−5.14, −3.89]), axonal regeneration markers NeuN cells/field 3.54, [2.65, 4.42]), NF200 4.88, [3.70, 6.05]), number Nissl bodies 1.89, [1.13, 2.65]), decreased astrogliosis marker GFAP −5.15, [−6.47, −3.82]). heterogeneity among primarily due variations transplantation doses, with increasing higher doses. Conclusion motor function by modulating inflammatory responses, reducing apoptosis, inhibiting astrogliosis, promoting regeneration. However, presence selection, performance, detection biases current may undermine evidence this study.

Язык: Английский

Процитировано

1