Quantification of Circulating Cell-Free DNA as a NETosis Marker in Trauma Patients with Type 2 Diabetes Mellitus DOI Creative Commons
Filiz Şahin, Regina Breinbauer, Caren Linnemann

и другие.

Methods and Protocols, Год журнала: 2025, Номер 8(2), С. 42 - 42

Опубликована: Апрель 16, 2025

Type 2 diabetes mellitus (T2DM) significantly impairs fracture healing, with neutrophils playing a crucial role in this process. In T2DM, these immune cells are over-activated, leading to the excessive release of neutrophil extracellular traps (NETs), increasing inflammation and hindering recovery. Thus, need for markers assess patients risk group arises. This study demonstrates that circulating cell-free DNA (cfDNA) can be efficiently quantified from serum samples by single-step qPCR used as marker NETosis. Our results revealed trauma T2DM have highest cfDNA levels, followed patients, healthy has lowest. The method shows strong correlations between neutrophil-specific such MPO, citH3, AZU1, α-defensin, highlighting its potential rapid indicator approach could allow timely interference high-risk ultimately improving healing outcomes reducing complications chronic inflammation, non-union fractures, diabetic foot ulcers.

Язык: Английский

Quantification of Circulating Cell-Free DNA as a NETosis Marker in Trauma Patients with Type 2 Diabetes Mellitus DOI Creative Commons
Filiz Şahin, Regina Breinbauer, Caren Linnemann

и другие.

Methods and Protocols, Год журнала: 2025, Номер 8(2), С. 42 - 42

Опубликована: Апрель 16, 2025

Type 2 diabetes mellitus (T2DM) significantly impairs fracture healing, with neutrophils playing a crucial role in this process. In T2DM, these immune cells are over-activated, leading to the excessive release of neutrophil extracellular traps (NETs), increasing inflammation and hindering recovery. Thus, need for markers assess patients risk group arises. This study demonstrates that circulating cell-free DNA (cfDNA) can be efficiently quantified from serum samples by single-step qPCR used as marker NETosis. Our results revealed trauma T2DM have highest cfDNA levels, followed patients, healthy has lowest. The method shows strong correlations between neutrophil-specific such MPO, citH3, AZU1, α-defensin, highlighting its potential rapid indicator approach could allow timely interference high-risk ultimately improving healing outcomes reducing complications chronic inflammation, non-union fractures, diabetic foot ulcers.

Язык: Английский

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