Integrative eQTL and Mendelian randomization analysis reveals key genetic markers in mesothelioma
Respiratory Research,
Год журнала:
2025,
Номер
26(1)
Опубликована: Апрель 13, 2025
Mesothelioma
is
a
rare
cancer
that
originates
from
the
pleura
and
peritoneum,
with
its
incidence
increasing
due
to
asbestos
exposure.
Patients
are
frequently
diagnosed
at
advanced
stages,
resulting
in
poor
survival
rates.
Therefore,
identification
of
molecular
markers
for
early
detection
diagnosis
essential.
Three
mesothelioma
datasets
were
downloaded
GEO
database
differential
gene
expression
analysis.
Instrumental
variables
(IVs)
identified
based
on
quantitative
trait
locus
(eQTL)
data
Mendelian
randomization
(MR)
analysis
using
Genome-Wide
Association
Study
(GWAS)
FINNGEN
database.
The
intersecting
genes
MR-identified
risk
differentially
expressed
as
key
co-expressed
mesothelioma.
Functional
enrichment
analyses,
including
Gene
Ontology
(GO),
Kyoto
Encyclopedia
Genes
Genomes
(KEGG),
Set
Enrichment
Analysis
(GSEA),
well
immune
cell
correlation
analysis,
performed
elucidate
roles
Additionally,
was
validated
independent
TCGA
datasets.
This
integrative
research
combining
multiple
databases
analytical
methods
established
robust
model
identifying
genes.
conducted
our
study
1608
By
these
192
MR
we
14
Notably,
MPZL1,
SOAT1,
TACC3,
CYBRD1
linked
high
mesothelioma,
while
TGFBR3,
NDRG2,
EPAS1,
CPA3,
MNDA,
PRKCD,
MTUS1,
ALOX15,
LRRN3,
ITGAM
associated
lower
risk.
These
found
be
enriched
pathways
superoxide
metabolism,
cycle
regulation,
proteasome
function,
all
which
development
Noteworthy
observations
included
significant
infiltration
M1
macrophages
CD4
+
T
cells
showing
positive
level
macrophage
infiltration.
Furthermore,
analyses
validation
set
confirmed
significance
eQTL
provides
evidence
causal
association
between
genetically.
disease
critical
implicated
correlations
biological
processes
infiltrated
related
Moreover,
lays
theoretical
foundation
further
into
mechanisms
potential
clinical
applications.
Язык: Английский
Salvia miltiorrhiza in osteoporosis: a review of its phytochemistry, traditional clinical uses and preclinical studies (2014–2024)
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Окт. 3, 2024
Osteoporosis
becomes
a
global
public
health
concern
due
to
its
rising
prevalence
and
substantial
impact
on
life
quality.
Salvia
miltiorrhiza
Bunge
(Salviae
Miltiorrhizae
Radix
et
Rhizoma,
SM)
has
been
firstly
recorded
in
Shen
Nong's
Herbal
Classic,
is
frequently
prescribed
conjunction
with
other
herbs
for
the
management
of
osteoporosis.
This
systematic
review
aims
comprehensively
analyze
recent
advances
SM
osteoporosis
traditional
Chinese
clinical
uses
preclinical
investigations.
Literature
encompassing
pertinent
studies
were
systematically
retrieved
across
multiple
databases,
including
PubMed,
Web
Science,
National
Knowledge
Infrastructure,
VIP
Database,
Biomedical
Database.
Original
investigations
spanning
from
February
2014
March
2024,
medicine
(TCM)
trials
studies,
employed
effects
actions
Thirty-eight
TCM
identified
employ
combination
primary
secondary
The
overall
efficacy
was
between
77%
96.67%.
Forty
investigate
and/or
ingredients
anti-osteoporosis
this
herb
may
be
attributed
inhibit
osteoclastogenesis/bone
resorption
promote
osteoblastogenesis/osteogenesis.
ethanol
extracts
(tanshinones)
by
inhibiting
MAPK/NF-κB/NFATc1
signaling
pathway
cathepsin
K-induced
collagen
degradation.
Both
water
(Sal
B
tanshinol)
contribute
osteoblastogenesis
promoting
osteogenesis
angiogenesis
via
activation
Wnt/β-catenin/VEGF
ERK/TAZ
pathways,
eliminating
ROS
production
targeting
Nrf2/ARE/HO-1
pathway.
In
conclusions,
offer
novel
strategy
management.
Well-designed
are
still
needed
evaluate
bone
remodeling.
Язык: Английский