Connexin46 in the nucleus of cancer cells: a possible role as transcription modulator DOI Creative Commons

Ainoa Fernández-Olivares,

Viviana P. Orellana,

Jesús Llanquinao

и другие.

Cell Communication and Signaling, Год журнала: 2025, Номер 23(1)

Опубликована: Март 27, 2025

Oncogenes drive cancer progression, but few are active exclusively in tumor cells. Connexins (Cxs), traditionally recognized as ion channel proteins, can localize to the nucleus and regulate gene expression, playing key roles both physiological pathological processes. Cx46, once thought be restricted eye lens, has been implicated growth, though its underlying mechanisms remain unclear. This study investigates nuclear presence of Cx46 cells potential role a transcriptional modulator. We employed ChIP-Seq, confocal immunofluorescence, protein purification assess localization DNA interactions. Functional assays were conducted evaluate effects on invasion, division, spheroid formation, mesenchymal marker expression. Single-point mutations molecular dynamics simulations used explore Cx46-DNA mRNA upregulation was found variety tumors compared adjacent healthy tissue. In HeLa cells, which do not express transfection promoted proliferation, invasion self-renewal capacity, stem cell traits features. Consistently, Sk-Mel-2, naturally reduced expression led decrease similar parameters. detected two forms, full length 46 kDa 30 fragment (GJA3-30 k), ChIP-Seq experiments revealed that binds at intergenic promoter regions, leading activation oncogenic pathways. Molecular suggest GJA3-30 k dimerizes RAD50-like structure, forming stable complexes. some cases nuclei multiple lines, including prostate, breast skin cancers. Our findings reveal novel for cancer, demonstrating function regulator therapeutic target.

Язык: Английский

The role of the nurse in smoking cessation: a critical essay DOI
Ana Elisa de Oliveira Alho, Mayza Luzia dos Santos Neves,

Carla Renata Silva Andrechuck

и другие.

Nursing & Care Open Access Journal, Год журнала: 2025, Номер 11(1), С. 22 - 25

Опубликована: Янв. 1, 2025

Objective: To reflect on the nurse's professional role in supporting smoking management from an educational perspective and to understand its implications educating people who use tobacco nursing staff. Methods: a theoretical-reflexive study based historical-cultural approach of Perceived Effects Substance Use Theory Smith. Results: The harmful effects cigarettes are well established world literature, despite harm that causes, nicotine is considered one most consumed drugs world. Tobacco chronic disease classified group mental behavioral disorders resulting psychoactive substances. For past decade, nurses have been engaged wide range interventions aimed at management. Nurse-led usually aim reduce control or achieve abstinence involve methods such as manuals, pamphlets, brochures, training printed materials, audio, video files besides consultations, replacement methods. Practice implications: Nurses played important smoking, promoting health education through individual guidance They proven their competence skill these issues.

Язык: Английский

Процитировано

0
Connexin46 in the nucleus of cancer cells: a possible role as transcription modulator DOI Creative Commons

Ainoa Fernández-Olivares,

Viviana P. Orellana,

Jesús Llanquinao

и другие.

Cell Communication and Signaling, Год журнала: 2025, Номер 23(1)

Опубликована: Март 27, 2025

Oncogenes drive cancer progression, but few are active exclusively in tumor cells. Connexins (Cxs), traditionally recognized as ion channel proteins, can localize to the nucleus and regulate gene expression, playing key roles both physiological pathological processes. Cx46, once thought be restricted eye lens, has been implicated growth, though its underlying mechanisms remain unclear. This study investigates nuclear presence of Cx46 cells potential role a transcriptional modulator. We employed ChIP-Seq, confocal immunofluorescence, protein purification assess localization DNA interactions. Functional assays were conducted evaluate effects on invasion, division, spheroid formation, mesenchymal marker expression. Single-point mutations molecular dynamics simulations used explore Cx46-DNA mRNA upregulation was found variety tumors compared adjacent healthy tissue. In HeLa cells, which do not express transfection promoted proliferation, invasion self-renewal capacity, stem cell traits features. Consistently, Sk-Mel-2, naturally reduced expression led decrease similar parameters. detected two forms, full length 46 kDa 30 fragment (GJA3-30 k), ChIP-Seq experiments revealed that binds at intergenic promoter regions, leading activation oncogenic pathways. Molecular suggest GJA3-30 k dimerizes RAD50-like structure, forming stable complexes. some cases nuclei multiple lines, including prostate, breast skin cancers. Our findings reveal novel for cancer, demonstrating function regulator therapeutic target.

Язык: Английский

Процитировано

0