Identification and functional characterization of prognosis-related ferroptosis-associated lncRNAs in colorectal cancer DOI Creative Commons
Xiaoxu Ge, Jiasheng Xu, Jinjie He

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Апрель 29, 2025

Colorectal cancer (CRC) is a significant global health burden, with current treatment strategies often limited by the TNM classification system's inability to fully capture tumor heterogeneity. This study aims explore biological functions and prognostic value of differentially expressed ferroptosis-related long non-coding RNAs (DEFRlncRNAs) in CRC. We utilized TCGA database identify DEFRlncRNAs associated CRC prognosis. Through multivariate Cox regression analysis, we constructed model selected three key lncRNAs: Lnc-SH2D3A-2, Lnc-LSS-1, Lnc-PEX11G-4. assessed their expression normal colonic epithelial cell lines using qPCR. Further functional assays included ferroptosis induction RSL3 Erastin, viability assessments, immunofluorescence staining for lipid peroxidation, Western blot analysis proteins. Our identified 15 significantly prognosis, Lnc-PEX11G-4 showing high basal lines. Knockdown Lnc-LSS-1 HT29 DLD1 cells resulted inhibition induced Erastin. The mechanism behind suppression knockdown may involve peroxidation upregulation GPX4 expression. DEFRlncRNAs, particularly Lnc-PEX11G-4, play crucial roles regulating These lncRNAs have potential as novel markers therapeutic targets, providing valuable insights personalized strategies.

Язык: Английский

Molecular mechanisms and clinical significance of perineural invasion in malignancies: the pivotal role of tumor-associated Schwann cells in cancer progression and metastasis DOI

Noura A. A. Ebrahim,

Soliman M. A. Soliman,

Maha Othman

и другие.

Medical Oncology, Год журнала: 2025, Номер 42(5)

Опубликована: Апрель 21, 2025

Язык: Английский

Процитировано

1
Identification and functional characterization of prognosis-related ferroptosis-associated lncRNAs in colorectal cancer DOI Creative Commons
Xiaoxu Ge, Jiasheng Xu, Jinjie He

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Апрель 29, 2025

Colorectal cancer (CRC) is a significant global health burden, with current treatment strategies often limited by the TNM classification system's inability to fully capture tumor heterogeneity. This study aims explore biological functions and prognostic value of differentially expressed ferroptosis-related long non-coding RNAs (DEFRlncRNAs) in CRC. We utilized TCGA database identify DEFRlncRNAs associated CRC prognosis. Through multivariate Cox regression analysis, we constructed model selected three key lncRNAs: Lnc-SH2D3A-2, Lnc-LSS-1, Lnc-PEX11G-4. assessed their expression normal colonic epithelial cell lines using qPCR. Further functional assays included ferroptosis induction RSL3 Erastin, viability assessments, immunofluorescence staining for lipid peroxidation, Western blot analysis proteins. Our identified 15 significantly prognosis, Lnc-PEX11G-4 showing high basal lines. Knockdown Lnc-LSS-1 HT29 DLD1 cells resulted inhibition induced Erastin. The mechanism behind suppression knockdown may involve peroxidation upregulation GPX4 expression. DEFRlncRNAs, particularly Lnc-PEX11G-4, play crucial roles regulating These lncRNAs have potential as novel markers therapeutic targets, providing valuable insights personalized strategies.

Язык: Английский

Процитировано

0