The lncrna HMMR-AS1 promotes the malignant progression of ovarian cancer cells by regulating the miR-627-3p/PTN axis DOI Creative Commons
Jinhua He, Fei Tian, Jie Li

и другие.

Journal of Ovarian Research, Год журнала: 2025, Номер 18(1)

Опубликована: Июнь 3, 2025

LncRNAs are crucial regulators of ovarian cancer, playing a significant role in malignant transformation and closely linked to poor prognosis. Therefore, it is investigate the impact lncRNAs on biological behavior cancer understand their underlying molecular mechanisms. Gene expression levels were measured using qRT-PCR. The cells was assessed through cell function assays. binding sites target genes predicted bioinformatics analysis, gene targeting relationships verified dual-luciferase reporter gene(DLRG) assay. Protein analyzed Western blotting. In cells, HMMR-AS1 PTN upregulated, whereas miR-627-3p downregulated. Cell experiments demonstrated that could enhance while exhibited opposite effect. DLRG assay indicated lncRNA directly targets miR-627-3p, with identified as miR-627-3p. blotting promoted protein, inhibited it. Furthermore, or partially reverse effects phenotype cells. summary, highly expressed plays carcinogenic by regulating miR-627-3p/PTN axis suggest potential strategy for treatment.

Язык: Английский

Molecular mechanisms and clinical significance of perineural invasion in malignancies: the pivotal role of tumor-associated Schwann cells in cancer progression and metastasis DOI

Noura A. A. Ebrahim,

Soliman M. A. Soliman,

Maha Othman

и другие.

Medical Oncology, Год журнала: 2025, Номер 42(5)

Опубликована: Апрель 21, 2025

Язык: Английский

Процитировано

0
The lncRNA HMMR-AS1 promotes the malignant progression of ovarian cancer cells by regulating the miR-627-3p/PTN axis DOI
Jinhua He, Fei Tian, Jie Li

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Апрель 21, 2025

Abstract Background Long non-coding RNAs (lncRNAs) are crucial regulators of ovarian cancer(OC), playing a significant role in malignant transformation and closely linked to poor prognosis. Therefore, it is investigate the impact lncRNAs on biological behavior cancer understand their underlying molecular mechanisms. Methods The binding sites target genes were predicted through bioinformatics analysis, gene expression levels measured using qRT-PCR. cells was assessed cell function assays. Gene targeting relationships verified dual-luciferase reporter gene(DLRG) assay. Protein analyzed Western blotting. Results Hyaluronan-mediated motility receptor antisense RNA1(HMMR-AS1) upregulated cells. Cell experiments demonstrated that HMMR-AS1 promotes behaviors DLRG targets miR-627-3p, which turn pleiotrophin(PTN). Furthermore, we discovered functions as competing endogenous RNA (ceRNA) for regulating PTN thereby promoting phenotypes Conclusions In summary, our study indicates lncRNA highly expressed plays carcinogenic role. Targeting may offer novel therapeutic strategy treating cancer.

Язык: Английский

Процитировано

0
The lncrna HMMR-AS1 promotes the malignant progression of ovarian cancer cells by regulating the miR-627-3p/PTN axis DOI Creative Commons
Jinhua He, Fei Tian, Jie Li

и другие.

Journal of Ovarian Research, Год журнала: 2025, Номер 18(1)

Опубликована: Июнь 3, 2025

LncRNAs are crucial regulators of ovarian cancer, playing a significant role in malignant transformation and closely linked to poor prognosis. Therefore, it is investigate the impact lncRNAs on biological behavior cancer understand their underlying molecular mechanisms. Gene expression levels were measured using qRT-PCR. The cells was assessed through cell function assays. binding sites target genes predicted bioinformatics analysis, gene targeting relationships verified dual-luciferase reporter gene(DLRG) assay. Protein analyzed Western blotting. In cells, HMMR-AS1 PTN upregulated, whereas miR-627-3p downregulated. Cell experiments demonstrated that could enhance while exhibited opposite effect. DLRG assay indicated lncRNA directly targets miR-627-3p, with identified as miR-627-3p. blotting promoted protein, inhibited it. Furthermore, or partially reverse effects phenotype cells. summary, highly expressed plays carcinogenic by regulating miR-627-3p/PTN axis suggest potential strategy for treatment.

Язык: Английский

Процитировано

0