Molecular mechanisms and clinical significance of perineural invasion in malignancies: the pivotal role of tumor-associated Schwann cells in cancer progression and metastasis
Noura A. A. Ebrahim,
Soliman M. A. Soliman,
Maha Othman
и другие.
Medical Oncology,
Год журнала:
2025,
Номер
42(5)
Опубликована: Апрель 21, 2025
Язык: Английский
The lncRNA HMMR-AS1 promotes the malignant progression of ovarian cancer cells by regulating the miR-627-3p/PTN axis
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 21, 2025
Abstract
Background
Long
non-coding
RNAs
(lncRNAs)
are
crucial
regulators
of
ovarian
cancer(OC),
playing
a
significant
role
in
malignant
transformation
and
closely
linked
to
poor
prognosis.
Therefore,
it
is
investigate
the
impact
lncRNAs
on
biological
behavior
cancer
understand
their
underlying
molecular
mechanisms.
Methods
The
binding
sites
target
genes
were
predicted
through
bioinformatics
analysis,
gene
expression
levels
measured
using
qRT-PCR.
cells
was
assessed
cell
function
assays.
Gene
targeting
relationships
verified
dual-luciferase
reporter
gene(DLRG)
assay.
Protein
analyzed
Western
blotting.
Results
Hyaluronan-mediated
motility
receptor
antisense
RNA1(HMMR-AS1)
upregulated
cells.
Cell
experiments
demonstrated
that
HMMR-AS1
promotes
behaviors
DLRG
targets
miR-627-3p,
which
turn
pleiotrophin(PTN).
Furthermore,
we
discovered
functions
as
competing
endogenous
RNA
(ceRNA)
for
regulating
PTN
thereby
promoting
phenotypes
Conclusions
In
summary,
our
study
indicates
lncRNA
highly
expressed
plays
carcinogenic
role.
Targeting
may
offer
novel
therapeutic
strategy
treating
cancer.
Язык: Английский
The lncrna HMMR-AS1 promotes the malignant progression of ovarian cancer cells by regulating the miR-627-3p/PTN axis
Journal of Ovarian Research,
Год журнала:
2025,
Номер
18(1)
Опубликована: Июнь 3, 2025
LncRNAs
are
crucial
regulators
of
ovarian
cancer,
playing
a
significant
role
in
malignant
transformation
and
closely
linked
to
poor
prognosis.
Therefore,
it
is
investigate
the
impact
lncRNAs
on
biological
behavior
cancer
understand
their
underlying
molecular
mechanisms.
Gene
expression
levels
were
measured
using
qRT-PCR.
The
cells
was
assessed
through
cell
function
assays.
binding
sites
target
genes
predicted
bioinformatics
analysis,
gene
targeting
relationships
verified
dual-luciferase
reporter
gene(DLRG)
assay.
Protein
analyzed
Western
blotting.
In
cells,
HMMR-AS1
PTN
upregulated,
whereas
miR-627-3p
downregulated.
Cell
experiments
demonstrated
that
could
enhance
while
exhibited
opposite
effect.
DLRG
assay
indicated
lncRNA
directly
targets
miR-627-3p,
with
identified
as
miR-627-3p.
blotting
promoted
protein,
inhibited
it.
Furthermore,
or
partially
reverse
effects
phenotype
cells.
summary,
highly
expressed
plays
carcinogenic
by
regulating
miR-627-3p/PTN
axis
suggest
potential
strategy
for
treatment.
Язык: Английский