Dissection of quantitative trait nucleotides and candidate genes associated with agronomic and yield-related traits under drought stress in rapeseed varieties: integration of genome-wide association study and transcriptomic analysis

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Март 19, 2024

Introduction An important strategy to combat yield loss challenge is the development of varieties with increased tolerance drought maintain production. Improvement crop under stress critical global food security. Methods In this study, we performed multiomics analysis in a collection 119 diverse rapeseed ( Brassica napus L.) dissect genetic control agronomic traits two watering regimes [well-watered (WW) and (DS)] for 3 years. DS treatment, irrigation continued till 50% pod stage, whereas WW condition, it was throughout whole growing season. Results The results genome-wide association study (GWAS) using 52,157 single-nucleotide polymorphisms (SNPs) revealed 1,281 SNPs associated traits. Six stable showed sequence variation flowering time between conditions across Three novel on chromosome C04 plant weight were located within tolerance-related gene ABCG16 , their pleiotropically effects seed per characterized. We identified C02 peak as signal time, harboring 52.77% SNPs. 288-kbps LD decay distance 2,232 candidate genes (CGs) CGs BIG1 - D CAND1 DRG3 PUP10 PUP21 involved phytohormone signaling pollen significant number, weight, grain conditions. By integrating GWAS RNA-seq, 215 promising developmental process, reproductive processes, cell wall organization, response stress. differentially expressed (DEGs) leaf contrasting accessions BIG1-D variation. Discussion our provide insights into improvement marker-assisted selection (MAS) breeding high-yield drought-tolerant varieties.

Язык: Английский

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Heat Stress and Plant–Biotic Interactions: Advances and Perspectives

Plants, Год журнала: 2024, Номер 13(15), С. 2022 - 2022

Опубликована: Июль 23, 2024

Climate change presents numerous challenges for agriculture, including frequent events of plant abiotic stresses such as elevated temperatures that lead to heat stress (HS). As the primary driving factor climate change, HS threatens global food security and biodiversity. In recent years, have negatively impacted physiology, reducing plant's ability maintain disease resistance resulting in lower crop yields. Plants must adapt their priorities toward defense mechanisms tolerate challenging environments. Furthermore, selective breeding long-term domestication higher yields made varieties vulnerable multiple stressors, making them more susceptible events. Studies on predict concurrent biotic will become severe future, potentially occurring simultaneously or sequentially. While most studies focused singular effects systems examine how plants respond specific stresses, simultaneous occurrence pose a growing threat agricultural productivity. Few explored interactions between plant-biotic interactions. Here, we aim shed light physiological molecular (bacteria, fungi, oomycetes, nematodes, insect pests, pollinators, weedy species, parasitic plants), well combined impact growth We also advances designing developing various strategies address multi-stress scenarios related factors.

Язык: Английский

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APETALA2/ethylene-responsive factors in higher plant and their roles in regulation of plant stress response

Critical Reviews in Biotechnology, Год журнала: 2024, Номер 44(8), С. 1533 - 1551

Опубликована: Янв. 24, 2024

Plants, anchored throughout their life cycles, face a unique set of challenges from fluctuating environments and pathogenic assaults. Central to adaptative mechanisms are transcription factors (TFs), particularly the AP2/ERF superfamily—one most extensive TF families plants. This family plays instrumental roles in orchestrating diverse biological processes ranging growth development secondary metabolism, notably, responses both biotic abiotic stresses. Distinguished by presence signature AP2 domain or its responsiveness ethylene signals, superfamily has become nexus research focus, with increasing literature elucidating multifaceted roles. review provides synoptic overview latest advancements on family, spanning taxonomy, structural nuances, prevalence higher plants, transcriptional post-transcriptional dynamics, intricate interplay DNA-binding target gene regulation. Special attention is accorded response factor B3 subgroup protein Pti5 role stress response, speculative insights into functionalities interaction matrix tomatoes. The overarching goal pave way for harnessing these TFs realms plant genetic enhancement novel germplasm development.

Язык: Английский

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Hotter, drier, CRISPR: the latest edit on climate change

Theoretical and Applied Genetics, Год журнала: 2021, Номер 134(6), С. 1691 - 1709

Опубликована: Янв. 8, 2021

Язык: Английский

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CRISPR–Cas9-based genetic engineering for crop improvement under drought stress

Bioengineered, Год журнала: 2021, Номер 12(1), С. 5814 - 5829

Опубликована: Янв. 1, 2021

In several parts of the world, prevalence and severity drought are predicted to increase, creating considerable pressure on global agricultural yield. Among all abiotic stresses, is anticipated produce most substantial impact soil biota plants, along with complex environmental impacts other ecological systems. Being sessile, plants tend be least resilient drought-induced osmotic stress, which reduces nutrient accessibility due heterogeneity limits access root system. Drought tolerance a quantitative trait regulated by multiple genes, it one challenging characteristics study classify. Fortunately, clustered regularly interspaced short palindromic repeat (CRISPR) technology has paved way as new frontier in crop improvement, thereby revolutionizing plant breeding. The application CRISPER systems proven groundbreaking across numerous biological fields, particularly biomedicine agriculture. present review highlights principle optimization CRISPR their implementation for terms tolerance, yield, domestication. Furthermore, we address ways innovative genome editing tools can help recognize modify novel genes coffering tolerance. We anticipate establishment effective strategies yield improvement water-limited regions through collaborative efforts near future.

Язык: Английский

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