Revolutionizing cancer treatment: enhancing CAR-T cell therapy with CRISPR/Cas9 gene editing technology

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Фев. 21, 2024

CAR-T cell therapy, a novel immunotherapy, has made significant breakthroughs in clinical practice, particularly treating B-cell-associated leukemia and lymphoma. However, it still faces challenges such as poor persistence, limited proliferation capacity, high manufacturing costs, suboptimal efficacy. CRISPR/Cas system, an efficient simple method for precise gene editing, offers new possibilities optimizing cells. It can increase the function of cells reduce costs. The combination CRISPR/Cas9 technology therapy may promote development this provide more effective personalized treatment cancer patients. Meanwhile, safety issues surrounding application require further research evaluation. Future should focus on improving accuracy to facilitate better therapy. This review focuses including eliminating inhibitory effect immune checkpoints, enhancing ability resist exhaustion, assisting construction universal cells, reducing costs security problems faced. objective is show revolutionary role researchers.

Язык: Английский

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Impacts of Climate Change and Mitigation Strategies for Some Abiotic and Biotic Constraints Influencing Fruit Growth and Quality

Plants, Год журнала: 2024, Номер 13(14), С. 1942 - 1942

Опубликована: Июль 15, 2024

Factors such as extreme temperatures, light radiation, and nutritional condition influence the physiological, biochemical, molecular processes associated with fruit development its quality. Besides abiotic stresses, biotic constraints can also affect growth Moreover, there be interactions between stressful conditions. However, it is challenging to predict generalize risks of climate change scenarios on seasonal patterns growth, development, yield, quality species because their responses are often highly complex involve changes at multiple levels. Advancements in genetic editing technologies hold great potential for agricultural sector, particularly enhancing crop traits. These improvements tailored meet consumer preferences, which crucial commercial success. Canopy management innovative training systems key factors that contribute maximizing yield efficiency improving quality, essential competitiveness orchards. creation habitats support pollinators a critical aspect sustainable agriculture, they play significant role production many crops, including fruits. Incorporating these strategies allows growers adapt changing conditions, increasingly important stability food production. By investing areas, stay ahead challenges opportunities industry, ultimately leading increased success profitability. In this review, we aim provide an updated overview current knowledge topic. We recommendations future research.

Язык: Английский

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CRISPR/Cas12a-based biosensors for environmental monitoring and diagnostics

Environmental Technology & Innovation, Год журнала: 2024, Номер 34, С. 103625 - 103625

Опубликована: Апрель 4, 2024

Contaminants, such as nucleic acids or toxic small molecules, threaten both human health and ecosystems when they infiltrate the environment. The precise highly sensitive identification of contaminants holds paramount importance across diverse domains, including safeguarding food integrity, facilitating clinical diagnostics, monitoring environmental conditions. Traditional methodologies, encompassing spectroscopy, chromatography, sequencing, metagenomics, have conventionally served pivotal roles in detection processes. Nevertheless, these methods encountered recurring challenges related to sensitivity, specificity, portability. This review focuses on groundbreaking CRISPR/Cas12-based biosensors. These biosensors leverage incredible precision programmability CRISPR/Cas system recognize specific targets. Here, we comprehensively assess fundamental mechanisms that enable detection, ranging from guide RNA design collateral cleavage. versatility CRISPR/Cas12 becomes evident through their applications. applications encompass medical safety, monitoring. transition conventional ultimately represents a significant milestone contaminant detection. By incorporating molecular biology, nanotechnology, bioinformatics, potential reshape landscape water CRIPSR-Cas diagnostics is transformative technology paves way for safer healthier future environment life.

Язык: Английский

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Actinomycete-Derived Pigments: A Path Toward Sustainable Industrial Colorants

Marine Drugs, Год журнала: 2025, Номер 23(1), С. 39 - 39

Опубликована: Янв. 13, 2025

Pigment production has a substantial negative impact on the environment, since mining for natural pigments causes ecosystem degradation, while synthetic pigments, derived from petrochemicals, generate toxic by-products that accumulate and persist in aquatic systems due to their resistance biodegradation. Despite these challenges, remain essential across numerous industries, including cosmetic, textile, food, automotive, paints coatings, plastics, packaging industries. In response growing consumer demand sustainable options, there is increasing interest eco-friendly alternatives, particularly bio-based algae, fungi, actinomycetes. This shift largely driven by options. For bio-pigments, actinomycetes, Streptomyces genus, have emerged as promising green source, aligning with global sustainability goals renewability biodegradability. Scale-up of yield optimization challenges been circumvented aid biotechnology advancements, genetic engineering innovative fermentation extraction methods, which enhanced bio-pigments’ viability cost-competitiveness. Actinomycete-derived successfully transitioned laboratory research commercialization, showcasing potential alternatives dyes. With pigment market valued at approximately USD 24.28 billion 2023, projected reach 36.58 2030, economic actinomycete extensive. review explores environmental advantages role modern industry, regulatory commercialization they face, highlighting importance solutions reduce our reliance conventional pigments. The successful can drive an industry-wide transition environmentally responsible offering benefits human health, safety, sustainability.

Язык: Английский

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Effective Delivery of CRISPR/dCas9-SAM for Multiplex Gene Activation Based on Mesoporous Silica Nanoparticles for Bladder Cancer Therapy

Acta Biomaterialia, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

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Recent Developments in CRISPR/Cas9 Genome-Editing Technology Related to Plant Disease Resistance and Abiotic Stress Tolerance

Biology, Год журнала: 2023, Номер 12(7), С. 1037 - 1037

Опубликована: Июль 22, 2023

The revolutionary CRISPR/Cas9 genome-editing technology has emerged as a powerful tool for plant improvement, offering unprecedented precision and efficiency in making targeted gene modifications. This practical approach to genome editing offers tremendous opportunities crop surpassing the capabilities of conventional breeding techniques. article provides an overview recent advancements challenges associated with application improvement. potential terms developing crops enhanced resistance biotic abiotic stresses is highlighted, examples genes edited confer disease resistance, drought tolerance, salt cold tolerance. Here, we also discuss importance off-target effects efforts made mitigate them, including use shorter single-guide RNAs dual Cas9 nickases. Furthermore, alternative delivery methods, such protein- RNA-based approaches, are explored, they could potentially avoid integration foreign DNA into genome, thus alleviating concerns related genetically modified organisms (GMOs). We emphasize significance accelerating processes, reducing time costs, enabling introduction desired traits at nucleotide level. As field continues evolve, it anticipated that will remain prominent adaptation challenging environmental conditions.

Язык: Английский

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Emerging frontiers in drug delivery with special focus on novel techniques for targeted therapies

Biomedicine & Pharmacotherapy, Год журнала: 2023, Номер 165, С. 115049 - 115049

Опубликована: Июнь 25, 2023

The management and treatment of disease are achieved via the use pharmacologically active substances or drugs. Drugs do not, however, have an intrinsic ability to be effective; rather, how well they work depends on administered supplied. Treatment a variety biological illnesses, such as autoimmune disorders, cancer, bacterial infections, requires effective drug delivery. Drug absorption, distribution, metabolism, duration therapeutic impact, pharmacokinetics, excretion, toxicity can all impacted by administration. Improved chemistry materials required for delivery concentration novel treatments specified targets within body, necessary time. This requirement is accompanied development new therapeutics. Formulating medication DDS promising strategy directly addressing numerous typical barriers adherence, frequent dosage, side effects, delayed beginning action. In current review, we give compendium controlled release subsequently highlight some newest developments in realm, with particular emphasis cutting-edge methods targeted therapy. each instance, outline obstacles efficient administration chemical material that allowing sector overcome these positive clinical impact.

Язык: Английский

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Multidrug-Resistant tuberculosis

Clinica Chimica Acta, Год журнала: 2024, Номер 559, С. 119701 - 119701

Опубликована: Май 1, 2024

Язык: Английский

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CRISPR/Cas genome editing in plants: mechanisms, applications, and overcoming bottlenecks

Functional & Integrative Genomics, Год журнала: 2024, Номер 24(2)

Опубликована: Март 5, 2024

Язык: Английский

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Advances in Nanoparticles as Non-Viral Vectors for Efficient Delivery of CRISPR/Cas9

Pharmaceutics, Год журнала: 2024, Номер 16(9), С. 1197 - 1197

Опубликована: Сен. 11, 2024

The clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system is a gene-editing technology. Nanoparticle delivery systems have attracted attention because of the limitations conventional viral vectors. In this review, we assess efficiency various nanoparticles, including lipid-based, polymer-based, inorganic, and extracellular vesicle-based systems, as non-viral vectors for CRISPR/Cas9 delivery. We discuss their advantages, limitations, current challenges. By summarizing recent advancements highlighting key strategies, review aims to provide comprehensive overview role in advancing technology clinical applications gene therapy.

Язык: Английский

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