Enhanced stability of anthocyanins and phages by titanium dioxide-loaded cyclodextrin-metal organic frameworks: application as a cherry tomato preservative
Food Bioscience,
Год журнала:
2025,
Номер
unknown, С. 106280 - 106280
Опубликована: Март 1, 2025
Язык: Английский
Trends in the research and development of peptide drug conjugates: artificial intelligence aided design
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 27, 2025
Peptide-drug
conjugates
(PDCs)
represent
an
emerging
class
of
targeted
therapeutic
agents
that
consist
small
molecular
drugs
coupled
to
multifunctional
peptides
through
cleavable
or
non-cleavable
linkers.
The
principal
advantage
PDCs
lies
in
their
capacity
deliver
diseased
tissues
at
increased
local
concentrations,
thereby
reducing
toxicity
and
mitigating
adverse
effects
by
limiting
damage
non-diseased
tissues.
Despite
the
increasing
number
being
developed
for
various
diseases,
advancements
remain
relatively
slow
due
several
development
constraints,
which
include
limited
available
linkers,
narrow
applications,
incomplete
evaluation
information
platforms
PDCs.
Marked
recent
Nobel
Prize
awarded
artificial
intelligence
(AI)
de
novo
protein
design
"protein
structure
prediction,"
AI
is
playing
increasingly
important
role
drug
discovery
development.
In
this
review,
we
summarize
developments
limitations
PDCs,
highlights
potential
revolutionizing
PDC.
Язык: Английский
Microfluidics, an effective tool for supporting phage display-A review
Analytica Chimica Acta,
Год журнала:
2024,
Номер
1326, С. 342978 - 342978
Опубликована: Июль 15, 2024
Язык: Английский
Engineered M13-Derived Bacteriophages Capable of Gold Nanoparticle Synthesis and Nanogold Manipulations
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(20), С. 11222 - 11222
Опубликована: Окт. 18, 2024
For
years,
gold
nanoparticles
(AuNPs)
have
been
widely
used
in
medicine
and
industry.
Although
various
experimental
procedures
reported
for
their
preparation
manipulation,
none
of
them
is
optimal
all
purposes.
In
this
work,
we
engineered
the
N-terminus
pIII
minor
coat
protein
bacteriophage
(phage)
M13
to
expose
a
novel
HLYLNTASTHLG
peptide
that
effectively
specifically
binds
gold.
addition
binding
gold,
phage
could
synthesize
spherical
AuNPs
20
nm
other
sizes
depending
on
reaction
conditions,
aggregate
them,
precipitate
from
colloid,
as
revealed
by
transmission
electron
microscopy
(TEM),
atomic
force
(AFM),
scanning
(SEM),
well
ultraviolet–visible
(UV–vis)
Fourier-transform
infrared
(FTIR)
spectroscopic
methods.
We
demonstrated
exposing
foreign
selected
phage-displayed
library
may
serve
sustainable
molecular
factory
both
synthesis
subsequent
overnight
ions
at
room
temperature
neutral
pH
absence
strong
reducing
agents,
such
commonly
NaBH4.
Taken
together,
results
suggest
potential
applicability
new,
vitro-identified
gold-binding
diverse
biomimetic
manipulations.
Язык: Английский
Affinity Peptide-Based Circularly Permuted Fluorescent Protein Biosensors for Non-Small Cell Lung Cancer Diagnosis
Sensors,
Год журнала:
2024,
Номер
24(24), С. 7899 - 7899
Опубликована: Дек. 11, 2024
Non-small
cell
lung
cancer
(NSCLC)
is
the
predominant
form
of
and
poses
a
significant
public
health
challenge.
Early
detection
crucial
for
improving
patient
outcomes,
with
serum
biomarkers
such
as
carcinoembryonic
antigen
(CEA),
squamous
carcinoma
(SCCAg),
cytokeratin
fragment
19
(CYFRA
21-1)
playing
critical
role
in
early
screening
pathological
classification
NSCLC.
However,
due
to
being
mainly
based
on
corresponding
antibody
binding
reactions,
existing
technologies
these
have
shortcomings
complex
operations,
high
false
positive
rates,
costs.
This
study
aimed
develop
new
methods
detecting
CEA,
SCCAg,
CYFRA
21-1
assist
diagnosis
Affinity
peptides
21-1,
respectively,
were
screened
by
phage
display
technology,
peptides’
affinities
determined
enzyme-linked
immunosorbent
assay
biolayer
interferometry.
Peptides
affinity
then
integrated
domains
into
biosensors
fusing
them
circularly
permuted
fluorescent
proteins
(cpFPs)
through
genetic
coding.
The
resulting
biosensors,
C4
biosensor
S1
Y3
demonstrated
robust
sensitivity
specificity
even
at
concentrations
low
1
ng/mL
their
respective
tumor
markers.
When
applied
clinical
samples
recalibrated
upper
limit
normal
concentrations,
exhibited
enhanced
NSCLC
diagnosis.
introduced
innovative
providing
highly
sensitive,
specific,
rapid,
cost-effective
diagnostic
alternative
that
could
significantly
improve
rates.
Язык: Английский