Ectopic overexpression of ShCBF1 and SlCBF1 in tomato suggests an alternative view of fruit responses to chilling stress postharvest DOI Creative Commons
Karin Albornoz, Jiaqi Zhou, Florence Zakharov

и другие.

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Авг. 5, 2024

Postharvest chilling injury (PCI) is a physiological disorder that often impairs tomato fruit ripening; this reduces quality and shelf-life, even accelerates spoilage at low temperatures. The CBF gene family confers cold tolerance in Arabidopsis thaliana , constitutive overexpression of increases vegetative tolerance, part by retarding growth, but, whether PCI unknown. We hypothesized CBF1 (OE) would be induced the increase resistance to PCI. high levels undergoing postharvest cloning it from S. lycopersicum habrochaites using stress-inducible RD29A promoter. Harvested were cold-stored (2.5°C) for up three weeks, then rewarmed 20°C days. Transgene upregulation was triggered during storage 8.6- 28.6-fold SlCBF1 -OE, between 3.1- 8.3-fold ShCBF1 -OE fruit, but developmental abnormalities absence induction visible. Remarkably, transgenic displayed worsening symptoms, i.e., failure ripen after rewarming, comparatively higher susceptibility decay relative wild-type (WT) lower total soluble solids, accumulation volatile compounds responsible off-odors. These symptoms correlated with levels. Transcriptomic analysis revealed ripening biotic abiotic stress responses altered fruit. Seedlings grown ‘chilled’ ‘non-chilled’ WT addition also exposed 0°C test their photosynthetic response injury. Chilled seedlings adjusted rates reduce oxidative damage; did not. Photosynthetic parameters similar 0°C, showed more severe photoinhibition than mirroring phenotypic observations. results suggest 1) accelerated deterioration storage, 2) Chilling acclimation fructus can seedling progeny tomato.

Язык: Английский

In vitro callus induction and identification of DNA variation in Etlingera elatior using Inter-simple sequence repeat (ISSR) markers DOI Creative Commons
Norsalsabila Mohd Rosli, Muhamad Yunus, Tamil Chelvan Meenakshi Sundram

и другие.

Ornamental Horticulture, Год журнала: 2025, Номер 31

Опубликована: Янв. 1, 2025

Abstract Etlingera elatior is a promising ornamental horticultural species with various purposes such as medicinal, antibacterial agent, culinary, ornamental, and floral arrangement. The increasing demand for more variation has led to the improvements of E. via tissue culture technology. Somaclonal helps overcome lack this due asexual propagation. aims study are induce callus shoot detect genetic variations using ISSR markers. results showed that Murashige & Skoog (MS) basal medium supplemented glucose 1.5 mg L-1 6-benzylaminopurine (BAP) 3 2,4-dichlorophenoxyacetic acid (2,4-D) produced significantly higher percentage, 50% after 20 weeks culture. friable calluses were then transferred induction media containing different concentrations BAP, 1-naphthaleneacetic (NAA) thidiazuron (TDZ). After 12 in media, root-like structure was observed masses T11 (0.1 NAA 0.3 TDZ). Seven primers used evaluate calluses. Seventy-two bands generated, which 51 polymorphic an average percentage 72%. Jaccard’s coefficient similarity values recorded between 0.3529 0.4762 exhibited level among In short, explants affected by auxin cytokinin induction. markers revealed occurrence during processes, suggesting potential generate new variants

Язык: Английский

Процитировано

0
Ectopic overexpression of ShCBF1 and SlCBF1 in tomato suggests an alternative view of fruit responses to chilling stress postharvest DOI Creative Commons
Karin Albornoz, Jiaqi Zhou, Florence Zakharov

и другие.

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Авг. 5, 2024

Postharvest chilling injury (PCI) is a physiological disorder that often impairs tomato fruit ripening; this reduces quality and shelf-life, even accelerates spoilage at low temperatures. The CBF gene family confers cold tolerance in Arabidopsis thaliana , constitutive overexpression of increases vegetative tolerance, part by retarding growth, but, whether PCI unknown. We hypothesized CBF1 (OE) would be induced the increase resistance to PCI. high levels undergoing postharvest cloning it from S. lycopersicum habrochaites using stress-inducible RD29A promoter. Harvested were cold-stored (2.5°C) for up three weeks, then rewarmed 20°C days. Transgene upregulation was triggered during storage 8.6- 28.6-fold SlCBF1 -OE, between 3.1- 8.3-fold ShCBF1 -OE fruit, but developmental abnormalities absence induction visible. Remarkably, transgenic displayed worsening symptoms, i.e., failure ripen after rewarming, comparatively higher susceptibility decay relative wild-type (WT) lower total soluble solids, accumulation volatile compounds responsible off-odors. These symptoms correlated with levels. Transcriptomic analysis revealed ripening biotic abiotic stress responses altered fruit. Seedlings grown ‘chilled’ ‘non-chilled’ WT addition also exposed 0°C test their photosynthetic response injury. Chilled seedlings adjusted rates reduce oxidative damage; did not. Photosynthetic parameters similar 0°C, showed more severe photoinhibition than mirroring phenotypic observations. results suggest 1) accelerated deterioration storage, 2) Chilling acclimation fructus can seedling progeny tomato.

Язык: Английский

Процитировано

0